Detection of Salmonella serotypes Enteritidis and Heidelberg by isothermal DNA amplification method
DOI:
https://doi.org/10.22491/2357-9730.140116Keywords:
Salmonella, Enteritidis, Heidelberg, LAMP, PCRAbstract
Introduction: Salmonella is a bacteria that causes human disease, usually due to the ingestion of animal foods. DNA analysis methods are alternatives for the direct detection of Salmonella and serotypes, with emphasis on the isothermal amplification of DNA using the LAMP technique (loop mediated isothermal amplification). Aim: To establish the detection of Salmonella serotypes Enteritidis and Heidelberg by the LAMP technique. Methods: A comparative analysis of the methods with serological and molecular tests (real-time PCR) was carried out in the evaluation of 40 isolates of nine important serotypes [14 (35%) of Enteritidis and 12 (30%) of Heidelberg] obtained from LACEN- LOL. Results: Fourteen Enteritidis and 12 Heidelberg isolates were detected only by PCR and LAMP tests specific for each serotype. Conclusion: Comparative analysis demonstrated full agreement between PCR and LAMP, indicating excellent analytical performance of the LAMP methods.
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