Tuberculous meningitis: evaluation of polymerase chain reaction (PCR) as a diagnostic tool – a pilot study.

Autores

  • Guilherme Geib HCPA
  • José Miguel Dora HCPA
  • Rafael Chakr HCPA
  • Fernanda de Paris HCPA
  • Alice Beatriz Mombach HCPA
  • Larissa Lutz HCPA
  • Carolina Fischinger Moura de Souza HCPA
  • Luciano Goldani HCPA/UFRGS

Palavras-chave:

Tuberculous meningitis, polymerase chain reaction, AIDS

Resumo

Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli in the cerebrospinal fluid by microscopy or culture. However the difficulty in detecting the organism poses a challenge to diagnosis. Use of polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis has been reported as a fast and accurate method, with several commercial kits available. As an alternative, some institutions have been developing inexpensive in-house assays. In our institution, we use an in-house PCR for tuberculosis. The performance of our PCR for the diagnosis of MTB meningitis was analyzed in 148 consecutive patients, using MTB culture as the gold standard. Sensitivity of cerebrospinal fluid PCR for the diagnosis of MTB meningitis was 50%, specificity was 98.6%, and concordance with culture was 96% (kappa = 0.52). The performance of our PCR is similar to that obtained with the available commercial kits.

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Publicado

2008-09-03

Como Citar

1.
Geib G, Dora JM, Chakr R, de Paris F, Mombach AB, Lutz L, Souza CFM de, Goldani L. Tuberculous meningitis: evaluation of polymerase chain reaction (PCR) as a diagnostic tool – a pilot study. Clin Biomed Res [Internet]. 3º de setembro de 2008 [citado 30º de setembro de 2022];28(2). Disponível em: https://seer.ufrgs.br/index.php/hcpa/article/view/5217

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