Effect of creatinine assay calibration on glomerular filtration rate prediction by MDRD equation

Débora Spessatto, Sandra Pinho Silveiro, Joíza Lins Camargo



Background: The evaluation of renal function should be performed with glomerular filtration rate (GFR) estimation employing the Modification of Diet in Renal Disease (MDRD) study equation, which includes age, gender, ethnicity and serum creatinine. However, creatinine methods require traceability with standardized methods. Objective: To analyse the impact of creatinine calibration on MDRD calculated GFR. Methods: 140 samples of plasma with creatinine values <2,0 mg/dl were analysed by Jaffé’s reaction with Creatinina Modular P (Roche ®; method A; reference) and Creatinina Advia 1650 (Bayer ®; method B; non-standardized). The results with the different methods were compared and aligned with standardized method through a conversion formula. MDRD GFR was estimated. Results: Values were higher for method B (1.03 ± 0.29 vs. 0.86 ± 0.32 mg/dl, P<0.001). This difference declined when methods were aligned with the equation y=1.07x -0.249, and the aligned values were 0,9 ± 0,31 mg/dl. Non-traceable creatinine methods misclassificaed chronic kidney disease in 10% more (false positive). This disagreement disappeared after the regression alignment. Conclusion: Creatinine method calibration has a large impact over the final results of serum creatinine and GFR. The alignment of the non-standardized results through conversion formulas is a reasonable alternative to harmonize serum creatinine results while waiting for the full implementation of international  standardization programs.


Taxa de filtração glomerular; TFG; creatinina sérica; Modification of Diet in Renal Disease, MDRD

Copyright (c)

ISSN: 2357-9730 





Apoio Financeiro:


Licença Creative Commons
The Clinical & Biomedical Research is licenced under Creative Commons Atribuição 4.0 Internacional.