Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test

Authors

  • Franciéli Pedrotti Rozales Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA)
  • Fernada de-Paris Serviço de Patologia Clínica, Unidade de Microbiologia e Biologia Molecular, HCPA, Porto Alegre, Brazil.
  • Alice Beatriz Mombach Pinheiro Machado Serviço de Patologia Clínica, Unidade de Microbiologia e Biologia Molecular, HCPA, Porto Alegre, Brazil.
  • Cintia Costi Centro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde (CDCT/FEPPS), Porto Alegre, Brazil.
  • Afonso Luis Barth Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil.

Keywords:

HCV, Diagnostic test, X-tail, Acute disease

Abstract

Infection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. 

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Published

2014-07-03

How to Cite

1.
Rozales FP, de-Paris F, Machado ABMP, Costi C, Barth AL. Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test. Clin Biomed Res [Internet]. 2014 Jul. 3 [cited 2025 Apr. 30];34(2). Available from: https://seer.ufrgs.br/index.php/hcpa/article/view/46502

Issue

Vol. 34 No. 2 (2014): Clinical and Biomedical Research

Section

Original Articles

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