Evaluation of different procedure involved in the Transcranial Direct Current Stimulation (tDCS) technique experimental application

Autores

  • Lauren Naomi Spezia Adachi Programa de Pós-Graduaçăo em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Carla de Oliveira Programa de Pós-Graduaçăo em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Rafael Vercelino Programa de Pós-Graduaçăo em Ciências da Reabilitação, Universidade Federal de Ciências da Saúde, Porto Alegre, Brasil.
  • Isabel Cristina de Macedo Programa de Pós-Graduaçăo em Ciências Biológicas: Fisiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Gabriela Laste Programa de Pós-Graduaçăo em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Alexandre Silva Quevedo Faculdade de Odontologia, Departamento de Cirurgia e Ortopedia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Vanessa Leal Scarabelot Laboratório de Farmacologia da Dor e Neuromodulação: Investigações Pré-Clínicas, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Wolnei Caumo Programa de Pós-Graduaçăo em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
  • Iraci Lucena da Silva Torres Laboratório de Farmacologia da Dor e Neuromodulação: Investigações Pré-Clínicas, Departamento de Farmacologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.

Palavras-chave:

tDCS, animal model, nociception, thermal hyperalgesia, stress restraint

Resumo

Introduction: The transcranial direct current stimulation (tDCS) is a non-invasive technique, which induces neuroplastic changes in the central nervous system of animals and humans. Furthermore, tDCS has been suggested as a therapeutic tool for pain management. The aim of this study was to standardize a non-invasive tDCS technique indexed by the nociceptive response of rats submitted to different conditions necessary to the tDCS application.

Method: 60-day-old male Wistar rats (n=65), divided into 6 groups: control(C); non-active sham (NAS); active-sham (AS); active-sham restrained (ASR); non-active sham restrained (NASR); active tDCS treatment. Animals received treatment during 30 seconds (sham-active) or 20 minutes (restraint and tDCS)/8 days. Nociceptive threshold was assessed by Hot Plate test at baseline, immediately and 24h after the first session, immediately and 24h after the last session. Variance analysis of repeated measurements followed by Bonferroni was performed for intra-group comparison.

Results: Physical restraint and 30 seconds stimulation (sham-tDCS) increased pain sensitivity (P≤0.05), and tDCS treatment was able to prevent the thermal hyperalgesia. Our original tDCS montage is similar to that used in the procedure with humans, because it is not an invasive technique. The electrodes are positioned on the head, and the animals are immobilized during the 20-minute treatment. As this procedure could involve behavior and neurochemical alterations due to stress induced by restriction (thus, it creates a research bias), we hypothesized that a 30-second electrical stimulus application (sham-tDCS) and the physical restriction used during tDCS treatment might alter nociceptive response in rats.

Conclusion: There are methodological limitations in the present tDCS-technique. Although active-tDCS treatment is able to prevent these harmful effects, interference of these factors has to be considered during the results’ analysis. Future adaptations of the tDCS-technique in rats are required to evaluate its therapeutic effects.

Keywords: tDCS; animal model; nociception; thermal hyperalgesia; stress restraint

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Publicado

2017-07-17

Como Citar

1.
Spezia Adachi LN, de Oliveira C, Vercelino R, de Macedo IC, Laste G, Quevedo AS, Scarabelot VL, Caumo W, da Silva Torres IL. Evaluation of different procedure involved in the Transcranial Direct Current Stimulation (tDCS) technique experimental application. Clin Biomed Res [Internet]. 17º de julho de 2017 [citado 10º de dezembro de 2022];37(2). Disponível em: https://seer.ufrgs.br/index.php/hcpa/article/view/71299

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