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VALIDATION OF A SIMPLE METHOD FOR SIMULTANEOUS DETERMINATION OF LIPOIC ACID AND RESVERATROL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • Irene C K Guerreiro Universidade Federal do Rio Grande do Sul
  • Samuel Davies
  • Silvia Guterres
  • Simone Jacobus Berlitz

DOI:

https://doi.org/10.22456/2527-2616.82578

Abstract

A high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed and validated for simultaneous determination of Resveratrol (RSV) and Lipoic Acid (LA). A C18 column was used with a mobile phase consisting of acetonitrile and 0.01M phosphoric acid (60:40). The detection wavelength was at 235 nm. The method was specific in the presence of pharmaceutical excipients widely used in solid dosage forms or lipid-core nanocapsules. The results demonstrated linearity between 5 and 50 µg/mL for RSV and 30 and 120 µg/mL for LA. The method presented precision and accuracy (RSD <5%). In addition, the developed method was considered robust. Therefore, the developed method can be applied successfully for simultaneous determination of RSV and LA in the proposed conditions, with a potential application to assay both drugs in several dosage forms.

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Published

14-09-2018

Versions

How to Cite

Guerreiro, I. C. K., Davies, S., Guterres, S., & Berlitz, S. J. (2018). VALIDATION OF A SIMPLE METHOD FOR SIMULTANEOUS DETERMINATION OF LIPOIC ACID AND RESVERATROL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. Drug Analytical Research, 2(1), p. 13–20. https://doi.org/10.22456/2527-2616.82578

Issue

Vol. 2 No. 1 (2018)

Section

ORIGINAL ARTICLES

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