Validation of a simple method for simultaneous determination of lipoic acid and resveratrol by high performance liquid chromatography

Irene C K Guerreiro; Samuel Davies; Silvia Guterres; Simone Jacobus Berlitz

doi:10.22456/2527-2616.82578

Authors

Irene C K Guerreiro Programa de Pós-Graduação em Ciências Farmacêuticas da Faculdade de Farmácia na Universidade Federal do Rio Grande do Sul
Samuel Davies Programa de Pós-Graduação em Ciências Farmacêuticas da Faculdade de Farmácia na Universidade Federal do Rio Grande do Sul
Silvia Guterres Programa de Pós-Graduação em Ciências Farmacêuticas da Faculdade de Farmácia na Universidade Federal do Rio Grande do Sul
Simone Jacobus Berlitz Programa de Pós-Graduação em Ciências Farmacêuticas da Faculdade de Farmácia na Universidade Federal do Rio Grande do Sul

DOI:

https://doi.org/10.22456/2527-2616.82578

Keywords:

Lipoic acid, resveratrol, HPLC, validation, simultaneous determination

Abstract

A high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed and validated for simultaneous determination of Resveratrol (RSV) and Lipoic Acid (LA). A C18 column was used with a mobile phase consisting of acetonitrile and 0.01M phosphoric acid (60:40). The detection wavelength was at 235 nm. The method was specific in the presence of pharmaceutical excipients widely used in solid dosage forms or lipid-core nanocapsules. The results demonstrated linearity between 5 and 50 µg/mL for RSV and 30 and 120 µg/mL for LA. The method presented precision and accuracy (RSD <5%). In addition, the developed method was considered robust. Therefore, the developed method can be applied successfully for simultaneous determination of RSV and LA in the proposed conditions, with a potential application to assay both drugs in several dosage forms.

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