This is an outdated version published on 08-03-2026. Read the most recent version.

Analytical method by liquid chromatography to assay piperine associated in nanoemulsions

Authors

  • Mariana Heldt Motta Programa de Pós-Graduação em Ciências Farmacêuticas na Universidade Federal de Santa Maria
  • Aline C. Schneider Programa de Pós-Graduação em Ciências Farmacêuticas na Universidade Federal de Santa Maria
  • Cristiane Franco Codevilla Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos na Universidade Federal de Santa Maria
  • Cristiano Menezes Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos na Universidade Federal de Santa Maria
  • Cristiane de Bona da Silva Programa de Pós-Graduação em Ciências Farmacêuticas na Universidade Federal de Santa Maria

DOI:

https://doi.org/10.22456/2527-2616.80366

Keywords:

Nanocarrier, Piper nigrum, Validation

Abstract

This work aimed to develop and validate a simple, fast and low cost analytical method for the quantification of the bioactive piperine in nanoemulsions by high performance liquid chromatography with UV detection. Nanoemulsions were prepared by spontaneous emulsification and their physicochemical properties were evaluated. Considering the chromatographic conditions, the mobile phase was composed by methanol:water (70:30, v/v), Gemini® C18 column, and UV detection at 343 nm. The method was linear in the concentration range of 5-50 μg mL-1 (r = 0.9999), specific, precise (repeatability of RSD 0.38 % and intermediate precision of RSD 1.11 %), accurate (101.3 %) and robust. Nanoemulsions showed nanometric droplet size, polidispersity index below 0.11 and negative zeta potential. The piperine content in the samples was 0.99 ± 0.01 mg mL-1. Regarding these features, the analytical conditions proposed in this work were adequate and effective to determine the piperine content in nanoemulsions.

Downloads

Download data is not yet available.

References

Meghawal M, Goswami TK; Phytother. Res. 2013, 27, 1121-1130. DOI: https://doi.org/10.1002/ptr.4972

Butt MS, Pasha I, Sultan MT, Randhawa MA, Saeed F, Ahmed W. Crit. Rev. Food Sci. Nutr. 2013, 53, 37-41. DOI: https://doi.org/10.1080/10408398.2011.571799

Polovka M, Suhaj M. Food Rev. Int. 2010, 26, 138-161. DOI: https://doi.org/10.1080/87559121003590227

Srinivasan K. Indian Food Ind. 2008, 27, 36-47.

de Souza EL, Stamford TLM, Lima EO, Trajano VN, Barbosa JMJr. Braz. Arch. Biol. Technol. 2005, 48, 549-558. DOI: https://doi.org/10.1590/S1516-89132005000500007

Srinivasan K. Food Rev. Int. 2005, 21, 167-188. DOI: https://doi.org/10.1081/FRI-200051872

Ferreira WS, Franklin TN, Lopes ND, de Lima MEF. Rev. Virtual Quim. 2012, 4, 208-224.

Thiel A, Buskens C, Woehrle T, Etheve S, Schoenmakers A, Fehr M, Beilstein P. Food Chem. Toxicol. 2014, 66, 350-357. DOI: https://doi.org/10.1016/j.fct.2014.01.056

McNamara FN, Randall A, Gunthorpe MJ. Br. J. Pharmacol. 2005, 144, 781-790. DOI: https://doi.org/10.1038/sj.bjp.0706040

Charcosset C, El-Harati A, Fessi H. J. Membr. Sci. 2005, 108, 115-120. DOI: https://doi.org/10.1016/j.jconrel.2005.07.023

Couvreur P, Barrat G, Fattal E, Legrand P, Vauthier C. Crit. Rev. Ther. Drug Carrier Syst. 2002, 19, 99-134. DOI: https://doi.org/10.1615/CritRevTherDrugCarrierSyst.v19.i2.10

Rawat M, Singh D, Saraf S. Biol. Pharma. Bull. 2006, 29, 1790-1798. DOI: https://doi.org/10.1248/bpb.29.1790

Schaffazick SR, Freitas LL, Pohlmann AR, Guterres SS. Quim. Nova. 2003, 26, 726-737. DOI: https://doi.org/10.1590/S0100-40422003000500017

Mishra B, Bhavesh PB, Tiwari S. Nanomedicine. 2010, 6, 9-24. DOI: https://doi.org/10.1016/j.nano.2009.04.008

Fontana MC, Coradini K, Guterres SS, Pohlmann AR, Beck RCR. J. Biomed. Nanotechnol. 2009, 5, 254-263. DOI: https://doi.org/10.1166/jbn.2009.1030

Ourique AF, Pohlmann AR, Guterres SS, Beck RCR. Int. J. Pharma. 2008, 352, 1-4. DOI: https://doi.org/10.1016/j.ijpharm.2007.12.035

Savian AL, Rodrigues D, Weber J, Ribeiro RF, Motta MH, Schaffazick SR, Adams AIH, Andrade DF, Beck RCR, Silva CB. Mater. Sci. Eng. C. 2015, 46, 69-76. DOI: https://doi.org/10.1016/j.msec.2014.10.011

Moorthi C, Krishnan K, Manavalan R, Kathiresan K. Asian Pac. J. Trop. Biomed. 2012, 2, 841-848. DOI: https://doi.org/10.1016/S2221-1691(12)60241-X

De Mey E, De Maere H, Dewulf L, Paelinck H, Sajewicz M, Fraeye I, Kowalska T. Eur. Food Res. Technol. 2014, 238, 477-484. DOI: https://doi.org/10.1007/s00217-013-2125-4

Gowda DV, Mugulil G, Jadhav AN, Nagraja P, Sarathchandraprakash, Babu UV, Paramesh R, Deshpande RD. Indo Am. J. Pharm. Res. 2014, 4, 4042-4046.

Su L, Yin JJ, Charles D, Zhow K, Moore J, Yu L. Food Chem. 2007, 100, 990-997. DOI: https://doi.org/10.1016/j.foodchem.2005.10.058

Kapoor IPS, Singh B, Singh G, DeHeluani CS, De-Lampasona MP, Catalan CAN. Journal of Agricultural and Food Chem. 2009, 57, 5358-5364.

Selvendiram K, Banu, SM, Sakthisekaram D. Clin. Chim. Acta. 2004, 350, 73-78.

Bouchemal K, Briançon S, Perrier E, Fessi H. Int. J. Pharm. 2004, 280, 241.

International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text and Methodology, Q2 (R1), 2005.

Kapoor IPS, Singh B, Singh G, DeHeluani CS, De-Lampasona MP, Catalan CAN. Journal of Agricultural and Food Chem. 2009, 57, 5358-5364. DOI: https://doi.org/10.1021/jf900642x

Selvendiram K, Banu, SM, Sakthisekaram D. Clin. Chim. Acta. 2004, 350, 73-78. DOI: https://doi.org/10.1016/j.cccn.2004.07.004

Bouchemal K, Briançon S, Perrier E, Fessi H. Int. J. Pharm. 2004, 280, 241. DOI: https://doi.org/10.1016/j.ijpharm.2004.05.016

International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text and Methodology, Q2 (R1), 2005.

Brazil. Agência Nacional de Vigilância Sanitária. 2017. Resolução RDC n° 166 de 27 de Julho de 2017. Dispõe sobre a validação de métodos analíticos e dá outras providências, Diário Oficial da União: Brasília.

Härter APG, Motta MH, Barros JG, Ribeiro RF, Adams AIH, Schaffazick SR, Silva CB. Quim. Nova. 2015, 37, 996-999

Lopes CE, Langoski G, Klein T, Ferrari PC, Farago PV. Braz. J. Pharm. Sci. 2017, 53, 1-9. DOI: https://doi.org/10.1590/s2175-97902017000215250

Salvia-Trujillo L, Sun Q, Um BH, Park Y, McClements DJ. J. Funct. Foods. 2015, 17, 293-304. DOI: https://doi.org/10.1016/j.jff.2015.05.035

Cattani VB, Fiel LA, Jäger A, Jäger E, Colomé LM, Uchoa F, Stefani V, Costa TD, Guterres SS, Pohlmann AR. Eur J Pharm Sci. 2010, 39, 116-124. DOI: https://doi.org/10.1016/j.ejps.2009.11.004

Pachauri M, Gupta ED, Ghosh PC. J Drug Deliv. Sci. Technol. 2015, 29, 269- 282. DOI: https://doi.org/10.1016/j.jddst.2015.08.009

Boddupalli BM, Masana P, Anisetti RN, Kallem SV, Madipoju B. Drug Inv. Today. 2013, 5, 212-215. DOI: https://doi.org/10.1016/j.dit.2013.05.011

Downloads

Published

14-09-2018 — Updated on 08-03-2026

Versions

How to Cite

Motta, M. H., C. Schneider, A., Codevilla, C. F., Menezes, C., & da Silva, C. de B. (2026). Analytical method by liquid chromatography to assay piperine associated in nanoemulsions . Drug Analytical Research, 2(1), 1–7. https://doi.org/10.22456/2527-2616.80366 (Original work published September 14, 2018)

Issue

Vol. 2 No. 1 (2018)

Section

ORIGINAL ARTICLES

License

Copyright (c) 2018 Drug Analytical Research

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The Copyright holder of manuscripts published is Drug Analytical Research. Authors who publish with this journal are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.


Licensing:

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.

Crossref
Scopus
Google Scholar
Europe PMC