Analytical method by liquid chromatography to assay piperine associated in nanoemulsions

Authors

  • Mariana Heldt Motta Programa de Pós-Graduação em Ciências Farmacêuticas na Universidade Federal de Santa Maria image/svg+xml
  • Aline C. Schneider Programa de Pós-Graduação em Ciências Farmacêuticas na Universidade Federal de Santa Maria image/svg+xml
  • Cristiane Franco Codevilla Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos na Universidade Federal de Santa Maria image/svg+xml
  • Cristiano Menezes Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos na Universidade Federal de Santa Maria image/svg+xml
  • Cristiane de Bona da Silva Programa de Pós-Graduação em Ciências Farmacêuticas na Universidade Federal de Santa Maria image/svg+xml

DOI:

https://doi.org/10.22456/2527-2616.80366

Keywords:

Nanocarrier, Piper nigrum, Validation

Abstract

This work aimed to develop and validate a simple, fast and low cost analytical method for the quantification of the bioactive piperine in nanoemulsions by high performance liquid chromatography with UV detection. Nanoemulsions were prepared by spontaneous emulsification and their physicochemical properties were evaluated. Considering the chromatographic conditions, the mobile phase was composed by methanol:water (70:30, v/v), Gemini® C18 column, and UV detection at 343 nm. The method was linear in the concentration range of 5-50 μg mL-1 (r = 0.9999), specific, precise (repeatability of RSD 0.38 % and intermediate precision of RSD 1.11 %), accurate (101.3 %) and robust. Nanoemulsions showed nanometric droplet size, polidispersity index below 0.11 and negative zeta potential. The piperine content in the samples was 0.99 ± 0.01 mg mL-1. Regarding these features, the analytical conditions proposed in this work were adequate and effective to determine the piperine content in nanoemulsions.

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Published

14-09-2018 — Updated on 11-03-2026

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How to Cite

Motta, M. H., C. Schneider, A., Codevilla, C. F., Menezes, C., & da Silva, C. de B. (2026). Analytical method by liquid chromatography to assay piperine associated in nanoemulsions . Drug Analytical Research, 2(1), 1–7. https://doi.org/10.22456/2527-2616.80366 (Original work published September 14, 2018)

Issue

Vol. 2 No. 1 (2018)

Section

ORIGINAL ARTICLES

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