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Determination of Arginine and Ascorbic acid in effervescent tablers by multidimensional chromatography

Authors

  • Andrea E. Rech School of Pharmacy and Health Science at Caxias do Sul University
    • Cristina P. Farina Laboratório Farmacêutico Vitamed em Caxias do Sul
      • Pamela C. Lukasewicz Ferreira Postgraduate Program in Pharmaceutical Sciences from the School of Pharmacy at Federal University of Rio Grande do Sul
        • Diogo Miron School of Pharmacy and Health Science at Caxias do Sul University; School of Pharmacy at Federal University of Rio Grande do Sul

          DOI:

          https://doi.org/10.22456/2527-2616.79222

          Keywords:

          Arginine, Ascorbic Acid, HPLC-UV, Tandem Columns, Vitamin C

          Abstract

          This paper describes a fast and direct method for quantification of arginine and ascorbic acid (vitamin C) in effervescent tablets. HPLC-UV multidimensional chromatography (RP18 and cyanopropyl columns) was developed and validated. Phosphate buffer 10 mMpH 3.4 was used as mobile phase. Satisfactory resolution between the drugs was obtained with analysis time less than 8.0 min. Method was linear in the ranges of 140-320 mg.mL-1 and 240-560 mg.mL-1 for arginine and ascorbic acid, respectively. Precision showed RSD <2.0%. Recovery was 99.5% and 100.0% for arginine and ascorbic acid, respectively. Robustness was confirmed through factorial analysis 22 (pH and mobile phase flow rate as factors).

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          References

          Bahri S, Zerrouk N, Aussel C, Moinard C, Crenn P, Curis E, et al. Citrulline: from metabolism to therapeutic use. Nutrition. 2013; 29(3): p. 479-84. DOI: https://doi.org/10.1016/j.nut.2012.07.002

          Böger RH. The pharmacodynamics of Larginine. Altern Ther Health Med. 2014; 20(3): p. 48-54.

          Lorin J, Zeller M, Guilland JC, Cottin Y, Vergely C, Rochette L. Arginine and nitric oxide synthase: regulatory mechanisms and cardiovascular aspects. Mol Nutr Food Res. 2014; 58(1): p. 101-16. DOI: https://doi.org/10.1002/mnfr.201300033

          Kishimoto Y, Saito N, Kurita K, Shimokado K, Maruyama N, Ishigami A. Ascorbic acid enhances the expression of type 1 and type 4 collagen and SVCT2 in cultured human skin fibroblasts. Biochem Biophys Res Commun. 2013; 430(2): p. 579-84. DOI: https://doi.org/10.1016/j.bbrc.2012.11.110

          May JM, Qu ZC, Nazarewicz R, Dikalov S. Ascorbic acid efficiently enhances neuronal synthesis of norepinephrine from dopamine. Brain Res Bull. 2013; 90: p. 35- 42. DOI: https://doi.org/10.1016/j.brainresbull.2012.09.009

          Lodhi GM, Latif R, Hussain MM, Naveed AK, Aslam M. Effect of ascorbic acid and alpha tocopherol supplementation on acute restraint stress induced changes in testosterone, corticosterone and nor epinephrine levels in male Sprague Dawley rats. J Ayub Med Coll Abbottabad. 2014; 26(1): p. 7-11.

          Chang Z, Huo L, Li P, Wu Y, Zhang P. Ascorbic acid provides protection for human chondrocytes against oxidative stress. Mol Med Rep. 2015; 12(5): p. 7086-92. DOI: https://doi.org/10.3892/mmr.2015.4231

          Zhang W, Kaye DM. Simultaneous determination of arginine and seven metabolites in plasma by reversed-phase liquid chromatography with a timecontrolled ortho-phthaldialdehyde precolumn derivatization. Anal. Biochem. 2004; 326(1): p. 87-92. DOI: https://doi.org/10.1016/j.ab.2003.11.006

          Markowski P, Baranowska I, Baranowski J. Simultaneous determination of larginine and 12 molecules participating in its metabolic cycle by gradient RP-HPLC method: Application to human urine samples. Anal. Chim. Acta. 2007; 605(2): p. 205-17. DOI: https://doi.org/10.1016/j.aca.2007.10.033

          0 Mao H, Wei W, Xiong W, Lu Y, Chen B, Liu Z. Simultaneous determination of lcitrulline and l-arginine in plasma by high performance liquid chromatography. Clin. Biochem. 2010; 43(13-14): p. 1141-7. DOI: https://doi.org/10.1016/j.clinbiochem.2010.05.017

          Alkaitis MS, Nardone G, Chertow JH, Ackerman HC. Resolution and quantification of arginine, monomethylarginine, asymmetric, dimethylarginine, and symmetric, dimethylarginine in plasma using HPLC with internal calibration. Biomedical Chromatography. 2016; 30: p. 294-300. DOI: https://doi.org/10.1002/bmc.3548

          Martens-Lobenhoffer J, Bode-Böger SM. Mass spectrometric quantification of Larginine and its pathway related substances in biofluids: The road to maturity. Journal of Chromatography B. 2014; 964: p. 89-102. DOI: https://doi.org/10.1016/j.jchromb.2013.10.030

          Servillo L, Giovane A, D’Onofrio N, Casale R, Cautela D, Castaldo D, et al. Determination of Homoarginine, Arginine, NMMA, ADMA, and SDMA in Biological Samples by HPLC-ESI-Mass Spectrometry. International Journal of Molecular Sciences. 2013; 14(10): p. 20131-20138. DOI: https://doi.org/10.3390/ijms141020131

          Huidobro AL, Rupérez FJ, Barbas C. Tandem column for the simultaneous determination of arginine, ibuprofen and related impurities by liquid chromatography. J Chromatogr. A. 2006; 1119: p. 238. DOI: https://doi.org/10.1016/j.chroma.2005.11.090

          Khan MI, Iqbal Z, Khan A. Simultaneous determination of ascorbic acid, aminothiols, and methionine in biological matrices using ion-pairing RP-HPLC coupled with electrochemical detector. Methods Mol Biol. 2015; 1208: p. 201-20. DOI: https://doi.org/10.1007/978-1-4939-1441-8_15

          Li X, Franke AA. Fast HPLC-ECD analysis of ascorbic acid, dehydroascorbic acid and uric acid. J Chromatogr B Analyt Technol Biomed Life Sci. 2009; 877(10): p. 853-6. DOI: https://doi.org/10.1016/j.jchromb.2009.02.008

          Ibrahim F, El-Enany N, El-Shaheny RN, Mikhail IE. Development and Validation of a New HPLC Method for the Simultaneous Determination of Paracetamol, Ascorbic Acid, and Pseudoephedrine HCl in their Coformulated Tablets. Application to in vitro Dissolution Testing. Anal Sci. 2015; 31(9): p. 943-7. DOI: https://doi.org/10.2116/analsci.31.943

          Wang YH, Avonto C, Avula B, Wang M, Rua D, Khan IA. Quantitative Determination of α-Arbutin, β-Arbutin, Kojic Acid, Nicotinamide, Hydroquinone, Resorcinol, 4-Methoxyphenol, 4- Ethoxyphenol, and Ascorbic Acid from Skin Whitening Products by HPLC-UV. J AOAC Int. 2015; 98(1): p. 5-12. DOI: https://doi.org/10.5740/jaoacint.14-123

          Chalcraft KR, McCarry BE. Tandem LC columns for the simultaneous retention of polar and nonpolar molecules in comprehensive metabolomics analysis. Journal of Separation Science. 2013; 36: p. 3478-85. DOI: https://doi.org/10.1002/jssc.201300779

          Link AJ WM. Analysis of protein composition using multidimensional chromatography and mass spectrometry. Curr Protoc Protein Sci. 2014; 78: p. 1-25. DOI: https://doi.org/10.1002/0471140864.ps2301s78

          ICH Harmonised Tripartite Guideline. Validation of analytical procedures: text and methodology Q2(R1).; 2005 [cited 2017 december

          Available from: http://www.ich.org/products/guidelines/q uality/quality-single/article/validation-ofanalytical-procedures-text-andmethodology.html. 22. Miron D, Lange A, Zimmer AR, Mayorga P, Schapoval EES. HPLC-DAD for the determination of three different classes of antifungals: method characterization, statistical approach, and application to a permeation study. Biomed. Chromatogr. 2014; 28: p. 1728–37. DOI: https://doi.org/10.1002/bmc.3213

          Ermer, J. Validation in pharmaceutical analysis. Part I: An integrated approach. Journal of Pharmaceutical and Biomedical Analysis. 2001; 24: p. 755–767. DOI: https://doi.org/10.1016/S0731-7085(00)00530-6

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          Published

          28-12-2017 — Updated on 07-03-2026

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          How to Cite

          E. Rech, A., P. Farina, C., C. Lukasewicz Ferreira, P., & Miron, D. (2026). Determination of Arginine and Ascorbic acid in effervescent tablers by multidimensional chromatography. Drug Analytical Research, 1(2), 44–52. https://doi.org/10.22456/2527-2616.79222 (Original work published December 28, 2017)

          Issue

          Vol. 1 No. 2 (2017)

          Section

          ORIGINAL ARTICLES

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