Determination of Arginine and Ascorbic acid in effervescent tablers by multidimensional chromatography

Andrea E. Rech; Cristina P. Farina; Pamela C. Lukasewicz Ferreira; Diogo Miron

doi:10.22456/2527-2616.79222

Authors

Andrea E. Rech School of Pharmacy and Health Science at Caxias do Sul University
Cristina P. Farina Laboratório Farmacêutico Vitamed em Caxias do Sul
Pamela C. Lukasewicz Ferreira Postgraduate Program in Pharmaceutical Sciences from the School of Pharmacy at Federal University of Rio Grande do Sul
Diogo Miron School of Pharmacy and Health Science at Caxias do Sul University; School of Pharmacy at Federal University of Rio Grande do Sul

DOI:

https://doi.org/10.22456/2527-2616.79222

Keywords:

Arginine, Ascorbic Acid, HPLC-UV, Tandem Columns, Vitamin C

Abstract

This paper describes a fast and direct method for quantification of arginine and ascorbic acid (vitamin C) in effervescent tablets. HPLC-UV multidimensional chromatography (RP18 and cyanopropyl columns) was developed and validated. Phosphate buffer 10 mMpH 3.4 was used as mobile phase. Satisfactory resolution between the drugs was obtained with analysis time less than 8.0 min. Method was linear in the ranges of 140-320 mg.mL^-1 and 240-560 mg.mL^-1 for arginine and ascorbic acid, respectively. Precision showed RSD <2.0%. Recovery was 99.5% and 100.0% for arginine and ascorbic acid, respectively. Robustness was confirmed through factorial analysis 2² (pH and mobile phase flow rate as factors).

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