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Development and validation of a high performance liquid chromatographic method determination of Zidovudine encapsulated in PCL nanoparticles

Authors

  • Milena Cristina Ribeiro Souza Magalhães Pharmaceutical and Biotechnological Development at Fundação Ezequiel Dias; School of Pharmacy at Federal University of Minas Gerais
    • Alisson Samuel Portes Caldeira Pharmaceutical and Biotechnological Development at Fundação Ezequiel Dias
      • Hanna de Sousa Rocha Almeida School of Pharmacy at Federal University of Minas Gerais
        • Sílvia Ligório Fialho Pharmaceutical and Biotechnological Development at Fundação Ezequiel Dias
          • Armando da Silva Cunha Junior School of Pharmacy at Federal University of Minas Gerais

            DOI:

            https://doi.org/10.22456/2527-2616.79216

            Keywords:

            zidovudine, nanoparticle, polycaprolactone, HPLC, validation

            Abstract

            A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of encapsulation efficiency of zidovudine in nanoparticules. The method was carried out in isocratic mode using 0.040M sodium acetate: methanol: acetonitrile: glacial acetic acid (880:100:20:2) as mobile phase, a C8 column at 25ºC and UV detection at 240 nm. The method was linear (r2 ˃ 0.99) over the range of 25.0-150.0 μg/mL, precise (RSD ˂ 5%), accurate (recovery = 100.5%), robust and selective. The validated HPLC-UV method can be successfully applied to determine the rate of zidovudine in nanoparticules.

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            Published

            28-12-2017 — Updated on 07-03-2026

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            How to Cite

            Ribeiro Souza Magalhães, M. C., Portes Caldeira, A. S., de Sousa Rocha Almeida, H., Ligório Fialho, S., & da Silva Cunha Junior, A. (2026). Development and validation of a high performance liquid chromatographic method determination of Zidovudine encapsulated in PCL nanoparticles. Drug Analytical Research, 1(2), 1–8. https://doi.org/10.22456/2527-2616.79216 (Original work published December 28, 2017)

            Issue

            Vol. 1 No. 2 (2017)

            Section

            ORIGINAL ARTICLES

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