Development and validation of a high performance liquid chromatographic method determination of Zidovudine encapsulated in PCL nanoparticles

Milena Cristina Ribeiro Souza Magalhães; Alisson Samuel Portes Caldeira; Hanna de Sousa Rocha Almeida; Sílvia Ligório Fialho; Armando da Silva Cunha Junior

doi:10.22456/2527-2616.79216

Authors

Milena Cristina Ribeiro Souza Magalhães Pharmaceutical and Biotechnological Development at Fundação Ezequiel Dias; School of Pharmacy at Federal University of Minas Gerais
Alisson Samuel Portes Caldeira Pharmaceutical and Biotechnological Development at Fundação Ezequiel Dias
Hanna de Sousa Rocha Almeida School of Pharmacy at Federal University of Minas Gerais
Sílvia Ligório Fialho Pharmaceutical and Biotechnological Development at Fundação Ezequiel Dias
Armando da Silva Cunha Junior School of Pharmacy at Federal University of Minas Gerais

DOI:

https://doi.org/10.22456/2527-2616.79216

Keywords:

zidovudine, nanoparticle, polycaprolactone, HPLC, validation

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of encapsulation efficiency of zidovudine in nanoparticules. The method was carried out in isocratic mode using 0.040M sodium acetate: methanol: acetonitrile: glacial acetic acid (880:100:20:2) as mobile phase, a C8 column at 25ºC and UV detection at 240 nm. The method was linear (r² ˃ 0.99) over the range of 25.0-150.0 μg/mL, precise (RSD ˂ 5%), accurate (recovery = 100.5%), robust and selective. The validated HPLC-UV method can be successfully applied to determine the rate of zidovudine in nanoparticules.

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