Bioanalysis of chrysin in human plasma and analytical stability using validated UV-spectrophotometric method:
a quality by design approach
DOI:
https://doi.org/10.22456/2527-2616.148193Keywords:
Bioanalysis, chrysin, quality by design, human plasma, UV-Visible spectrophotometer.Abstract
Chrysin is a flavonoid with many biological and medicinal activities, such as anti-cancer, anti-inflammatory, anti-diabetic,
and antioxidant properties, and has medicinal value. Based on the Quality by Design approach, the present study used a
UV-spectrophotometer to develop a bioanalytical method for chrysin in human plasma. The validation of the method was
performed as per ICH M10 guidelines. The technique was performed using methanol as a solvent and the spectrum was
recorded at a wavelength of 370 nm. The spiked chrysin from plasma was extracted using the protein precipitation method.
The developed method was linear with an r2 value of 0.9996 over a concentration range of 4-20 μg/ml. The results of all
validation parameters are found to be within the accepted limits with a % RSD value of less than 2% and the % recovery
was greater than 95%. The % RSD and % recovery results confirmed that the method was precise and accurate in the study.
LOD and LOQ values in plasma samples were found to be 0.47 μg/ml and 1.43 μg/ml respectively. The stability studies
like freeze-thaw, and short-term stability studies were also performed and proved the reliability of the method. Therefore,
the developed bioanalytical method can effectively estimate chrysin in plasma samples within its sensitivity limits.
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