Bioanalysis of chrysin in human plasma and analytical stability using validated UV-spectrophotometric method: a quality by design approach

Rohini Pargonda Patil; Shailendra Sanjay Suryawanshi; Mahesh Shivabasappa Palled

doi:10.22456/2527-2616.148193

Authors

Rohini Pargonda Patil Department of Pharmaceutical Chemistry - KLE College of Pharmacy https://orcid.org/0009-0005-9987-2312
Shailendra Sanjay Suryawanshi Department of Pharmaceutical Chemistry - KLE College of Pharmacy https://orcid.org/0000-0003-1646-8815
Mahesh Shivabasappa Palled Department of Pharmaceutical Chemistry - KLE College of Pharmacy https://orcid.org/0000-0002-9223-5653

DOI:

https://doi.org/10.22456/2527-2616.148193

Keywords:

Bioanalysis, Chrysin, Quality by design, Human plasma, UV-Visible Spectrophotometer

Abstract

Chrysin is a flavonoid with many biological and medicinal activities, such as anti-cancer, anti-inflammatory, anti-diabetic, and antioxidant properties, and has medicinal value. Based on the Quality by Design approach, the present study used a UV-spectrophotometer to develop a bioanalytical method for chrysin in human plasma. The validation of the method was performed as per ICH M10 guidelines. The technique was performed using methanol as a solvent and the spectrum was recorded at a wavelength of 370 nm. The spiked chrysin from plasma was extracted using the protein precipitation method. The developed method was linear with an r2 value of 0.9996 over a concentration range of 4-20 μg/ml. The results of all validation parameters are found to be within the accepted limits with a % RSD value of less than 2% and the % recovery was greater than 95%. The % RSD and % recovery results confirmed that the method was precise and accurate in the study. LOD and LOQ values in plasma samples were found to be 0.47 μg/ml and 1.43 μg/ml respectively. The stability studies like freeze-thaw, and short-term stability studies were also performed and proved the reliability of the method. Therefore, the developed bioanalytical method can effectively estimate chrysin in plasma samples within its sensitivity limits.

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