LC method for quantitative determination of Ciprofloxacin in ophthalmic ointment

Authors

  • Lionel Salem Aza-Gnandji
  • Livia Maronesi Bueno
  • Tércio Paschke Oppe
  • Elfrides Eva Scherman Schapoval

DOI:

https://doi.org/10.22456/2527-2616.118365

Abstract

The objective of the study was to validate an analytical method for the quantification of ciprofloxacin in ophthalmic ointment by high performance liquid chromatography. The chromatographic separation of ciprofloxacin hydrochloride was achieved on a Thermo hypersil Gold C18 column using UV detection at 278 nm. The optimized mobile phase consisted of a mixture of 0.025 M phosphoric acid with a pH previously adjusted with triethylamine to 3.0 and acetonitrile (85:15, v / v). The validation method yielded good results demonstrated statistically that the method was linear, precise, accurate, specific and robust. No interference from any components of the pharmaceutical dosage forms was observed.

 

Downloads

Download data is not yet available.

Downloads

Published

2021-12-15

Issue

Section

ORIGINAL ARTICLES