New, green and miniaturized analytical method for determination of cefadroxil monohydrate in capsules

Bianca Aparecida de Marco; Ana Carolina Kogawa; Hérida Regina Nunes Salgado

doi:10.22456/2527-2616.91086

Authors

Bianca Aparecida de Marco São Paulo State University (UNESP) - School of Pharmaceutical Sciences
Ana Carolina Kogawa São Paulo State University (UNESP) - School of Pharmaceutical Sciences
Hérida Regina Nunes Salgado São Paulo State University (UNESP) - School of Pharmaceutical Sciences

DOI:

https://doi.org/10.22456/2527-2616.91086

Keywords:

cefadroxil monohydrate, capsules, spectrophotometric method in the visible region, miniaturized method, green analytical chemistry

Abstract

Cefadroxil, an oral antimicrobial, presents few techniques optimized for the reduction of solvents and toxic residues and/or non-use of them. So, a quantitative, new and miniaturized method for determination of cefadroxil monohydrate in capsules has been developed and validated by spectrophotometric method in the visible region according to the international guidelines. The analyzes were performed using microplates containing 96 wells, 1 % of phenolphthalein and sodium hydroxide 0.1 M as reagent at 552 nm. The method was (i) linear in the range of 15-115 µg mL^-1, (ii) selective when comparing standard, sample, adjuvants and color reagent, (iii) precise with deviations below 4 %, (iv) accurate when comparing the proposed method with the HPLC method, (v) robusts by making small and deliberate modifications to the method, (vi) besides being fast, low cost, eco-friendly and generates minimal amount of waste. The method can be applied to the routine quality control of cefadroxil monohydrate in capsules and an effective and accessible alternative that contemplates the concepts of current and sustainable green analytical chemistry.

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