Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation

Morgana Souza Marques; Fernando Dal Pont Morisso; Fernanda Poletto; Irene Clemes Külkamp Guerreiro

doi:10.22456/2527-2616.111454

Authors

Morgana Souza Marques Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul
Fernando Dal Pont Morisso Pós-Graduação em Tecnologia de Materiais e Processos Industriais - Universidade Feevale
Fernanda Poletto Departamento de Química Orgânica, Instituto de Química - Universidade Federal do Rio Grande do Sul
Irene Clemes Külkamp Guerreiro Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Rio Grande do Sul

DOI:

https://doi.org/10.22456/2527-2616.111454

Keywords:

Pyrazinamide, Rifampicin, UV spectrophotometry, derivative spectrophotometry

Abstract

The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous determination of pyrazinamide (PYZ) and rifampicin (RIF). The spectrophotometric method was evaluated according to validation guidelines for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness. The first-derivative spectra were obtained and by the zerocrossing point, the wavelength 247 nm and 365 nm were selected for PYZ and RIF quantification, respectively. No interference from cubosome excipients was detected in the proposed method. The results demonstrated linearity in a range of 4.0 – 12.0 µg/mL with an adequate correlation coefficient for both drugs. The intra and inter-day precision results (RSD < 5%) indicated the reproducibility of the method. The accuracy data showed satisfactory results (RSD < 5%) from recovery test. In addition, the robustness results showed that the PYZ and RIF content were unaffected by the solvent alteration of methanol to methanol:water (99:1, v/v). The derivative ultraviolet spectrophotometric method proved to be an excellent strategy for simultaneous determination of PYZ and RIF.

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