Cannabis sativa: A systematic review of plant analysis
DOI:
https://doi.org/10.22456/2527-2616.73676Keywords:
Cannabis sativa, marijuana, cannabinoids detection, plant analysis and analytical methodsAbstract
Cannabis has been the most widely used illicit drug worldwide throughout many years. Reports from different countries indicate that the potency of cannabis preparation has been increasing, as well as the ratio of tetrahydrocannabinol/cannabidiol has been changing. The high consumption along with the changing chemical profile of the drug has led increasingly to the interest in researching the cannabis plant. This article reviews available literature on the analytical methods currently used for the detection and quantification of cannabinoids in cannabis plant. The papers were screened by two researchers independently and following a pre-specified protocol. The systematic review of the literature allowed to include 42 citations on cannabis plant analysis. The analytical methods for cannabis material published in the included articles of this systematic review showed a lack of relevant information of the development of methods on GC and LC analysis and the limits of detection and quantification of mass detectors.
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References
UNODC - United Nations Office on Drugs and
Crime (2016) World Drug Report 2016.
http://www.UNODC.org/doc/wdr2016/WORLD_D
RUG_REPORT_2016_w eb.pdf (accessed on 04
September 2016).
WHO - World Health Organization (2016). The
health and social effects of nonmedical cannabis
use.
http://www.WHO.int/substance_abusepublications/
ms bcannabis.pdf (accessed on 04 September 2016).
Bruci, Z., Papoutsis, I., Athanaselis, S., Nikolaou,
P., Pazari, E., Spiliopoulou, C., Vyshka, G. (2012)
First systematic evaluation of the potency of
Cannabis sativa plants grown in Albania, Forensic
Science International, 222, 40–46.
Swift, W., Wong, A., Li, K.M., Arnold, J.C.,
McGregor, I.S. (2013) Analysis of Cannabis
Seizures in NSW, Australia: Cannabis Potency and
Cannabinoid Profile, PLoS ONE, 8, 1–9.
Aizpurua-Olaizola, O., Soydaner, U., Öztürk, E.,
Schibano, D., Simsir, Y., Navarro, P., Etxebarria,
N., Usobiaga, A. (2016) Evolution of the
Cannabinoid and Terpene Content during the
Growth of Cannabis sativa Plants from Different
Chemotypes, Journal of Natural Products, 79, 324–
331.
De Backer, B., Maebe, K., Verstraete, A.G.,
Charlier, C. (2012) Evolution of the Content of THC
and Other Major Cannabinoids in Drug-Type
Cannabis Cuttings and Seedlings During Growth of
Plants, Forensic Science International, 57, 918–922.
Broséus, J., Anglada, F., Esseiva, P. (2010). The
differentiation of fibre- and drug type Cannabis
seedlings by gas chromatography/mass spectrometry
and chemometric tools, Forensic Science
International, 200, 87–92.
Mariotti, K.C., Marcelo, M.C.A., Ortiz, R.S.,
Borille, B.T., Reis, M., Fett, M.S., Ferrão, M.F.,
Limberger, R.P. (2016) Seized cannabis seeds
cultivated in greenhouse: A chemical study by gas
chromatography–mass spectrometry and
chemometric analysis, Science & Justice, 56, 35–41.
UNODC - United Nations Office on Drugs and
Crime (2009) Recommended methods for the
identification and analysis of cannabis and cannabis
products.
https://www.UNODC.org/documents/scientific/STNAR-
40-Ebook.pdf (accessed on 04 September
2016).
Trofin, I. G., Vlad, C.C., Noja, V.V., Dabija, G.
(2012) Identification and Characterization of Special
Types of Herbal Cannabis, UPB Scientific Bulletin,
74, 119–130.
Taschwer, M., Schmid, M.G. (2015) Determination
of the relative percentage distribution of THCA and
Δ9-THC in herbal cannabis seized in Austria -
Impact of different storage temperatures on stability,
Forensic Science International, 254, 167–171.
Cadola, L., Broséus, J., Esseiva, P. (2013) Chemical
profiling of different hashish seizures by gas
chromatography–mass spectrometry and statistical
methodology: A case report, Forensic Science
International, 232, 24–27.
Pandohee, J., Holland, B.J., Li, B., Tsuzuki, T.,
Stevenson, P.G., Barnett, N.W., Pearson, J.R.,
Jones, O.A.H., Conlan, X.A. (2015) Screening of
cannabinoids in industrial-grade hemp using twodimensional
liquid chromatography coupled with
acid potassium permanganate chemiluminescence
detection, Journal of Separation Science, 38, 2024–
2032.
Ambach, L., Penitschka, F., Broillet, A., König, S.,
Weinmann, W., Bernhard, W. (2014) Simultaneous
quantification of delta-9-THC, THC-acid A, CBN
and CBD in seized drugs using HPLC-DAD,
Forensic Science International, 243, 107–111.
Rotherham, D., Harbison, S.A. (2011)
Differentiation of drug and non-drug Cannabis using
a single nucleotide polymorphism (SNP) assay,
Forensic Science International, 207, 193–197.
Cascini, F., Passerotti, S., Martello, S. (2012) A
real-time PCR assay for the relative quantification
of the tetrahydrocannabinolic acid (THCA) synthase
gene in herbal Cannabis samples, Forensic Science
International, 217, 134–138.
Moher, D., Liberati, A., Tetzlaff, J., Altman, D.G.,
Group, P. (2010) Preferred reporting items for
systematic reviews and meta-analyses: the PRISMA
statement, International Journal of Surgery, 8, 336–
341.
Small, E., Cronquist, A. (1976) A Practical and
Natural Taxonomy for Cannabis, Taxon, 25, 405–
435.
Small, E. (2016) Evolution and Classification of
Cannabis sativa (Marijuana, Hemp) in Relation to
Human Utilization, Botanical Review, 81, 189–294.
Sawler, J., Stout, J.M., Gardner, K.M.., Hudson, D.,
Vidmar, J., Butler, L., Page, J.E., Myles, S. (2015)
The Genetic Structure of Marijuana and Hemp,
PLoS ONE, 10, 1–9.
Amaducci, S., Scordia, D., Liu, F.H., Zhang, Q.,
Guo, H., Testa, G., Cosentino, S.L. (2015) Key
cultivation techniques for hemp in Europe and
China, Industrial Crops and Products, 68, 2–16.
Gloss, D. (2015) An Overview of Products and Bias
in Research, Neurotherapeutics, 12, 731–734.
Salentijn, E.M.J., Zhang, Q., Amaducci, S., Yang,
M., Trindade, L.M. (2015) New developments in
fiber hemp (Cannabis sativa L.) breeding, Industrial
Crops and Products, 68, 32–41.
Elsohly, M.A., Slade, D. (2005) Chemical
constituents of marijuana: The complex mixture of
natural cannabinoids, Life Sciences, 78, 539–548.
Radwan, M.M., ElSohly, M.A., El-Alfy, A.T.,
Ahmed, S.A., Slade, D., Husni, A.S., Manly, S.P.,
Wilson L., Seale S., Cutler, S.J., Ross, S.A. (2015)
Isolation and Pharmacological Evaluation of Minor
Cannabinoids from High-Potency Cannabis sativa,
Journal of Natural Products, 78, 1271–1276.
Russo, E.B. (2011) Taming THC: potential cannabis
synergy and phytocannabinoid-terpenoid entourage
effects, British Journal of Pharmacology, 163,
1344–1364.
Small, E., Naraine, S.G.U. (2016) Size matters:
evolution of large drug-secreting resin glands in
elite pharmaceutical strains of Cannabis sativa
(marijuana), Genetic Resources and Crop Evolution,
63, 349–359.
Onofri, C., De Meijer, E.P.M., Mandolino, G.
(2015) Sequence heterogeneity of cannabidiolicand
tetrahydrocannabinolic acid-synthase in
Cannabis sativa L. and its relationship with
chemical phenotype, Phytochemistry, 116, 57–68.
Rogeberg, O., Elvik, R. (2016). The effects of
cannabis intoxication on motor vehicle collision
revisited and revised. Addiction, 111, 1348–1359.
Evans, D.G. (2013). The Economic Impacts Of
Marijuana Legalization, The Journal of Global Drug
Policy and Practice, 1–39.
ElSohly, M.A., Mehmedic, Z., Foster, S. Gon, C.,
Chandra, S., Church, J.C. (2016) Changes in
cannabis potency over the last two decades (1995-
2014) - Analysis of current data in the United States.
Biological Psychiatry, 79, 613–619.
Crippa, J.A., Lacerda, L.T.A., Amaro, E., Busatto
Filho, G., Zuardi, A.W., Bressan, R.A. (2005)
Efeitos cerebrais da maconha: resultados dos
estudos de neuroimagem. Revista Brasileira de
Psiquiatria, 27, 70–78.
Freeman, T.P., Winstock, A.R. (2015) Examining
the profile of high-potency cannabis and its
associatiion with severety of cannabis dependence,
Psychological Medicine, 45, 3181–3189.
Di Forti, M., Marconi, A., Carra, E., Fraletta, S.,
Trotta, A., Bonomo, M., Murray, R.M. (2015)
Proportion of patients in soth London with first
episode psychosis attributable to use of highpotency
cannabis: a case control study, The Lancet
Psychiatry, 2, 233–238.
Hall, W. (2015) What has research ove the past two
decadesrevelated about the adverse health effects of
recreational cannabis use?, Addiction, 110, 19–35.
Tipparat, P., Kunkaew, W., Julsrigival, S.,
Pinmanee, S., Natakankitkul, S. (2014)
Classification of Cannabis Plants Grown in Northern
Thailand using Physico-Chemical Properties,
Journal of Natural Sciences Research, 4, 46–54.
Vanhove, W., Van Damme, P., Meert, N. (2011)
Factors determining yield and quality of illicit
indoor cannabis (Cannabis spp.) production,
Forensic Science International, 212, 158–163.
Mander, L., Liu, H-W., (2010) Comprehensive
Natural Products II: Chemistry and Biology,
Volume 1, 1st Edition, Elsevier Science.
Ahmad, U.K., Muniandy, Y., Hassan, M.S. (2014)
Physical Analysis and Chemical Profiling of Illicit Herbal Cannabis using Multivariate Analysis,
Malaysian Journal of Forensic Sciences, 5, 26–34.
Giese, M.W., Lewis, M.A., Giese, L., Smith, K.M.
(2015) Development and Validation of a Reliable
and Robust Method for the Analysis of
Cannabinoids and Terpenes in Cannabis, Drug
Formulations and Clinical Methods, 98, 1503–1522.
Hoffmann, E., Stroobant, V. (2007) Mass
Spectrometry: Principles and Applications, 3rd
Edition, John Wiley & Sons Inc, London.
Nikolaev, E.N., Kostyukevich, Y.I., Vladimirov,
G.N. (2016) Fourier transform ion cyclotron
resonance (ft icr) mass spectrometry: theory and
simulations, Mass Spectrometry Reviews, 35, 219–
259.
Chandra, S., Lata, H., Mehmedic, Z., Khan, I.A.,
ElSohly, M.A. (2010) Assessment of Cannabinoids
Content in Micropropa- gated Plants of Cannabis
sativa and Their Comparison with Conventionally
Propagated Plants and Mother Plant during
Developmental Stages of Growth, Planta Medica,
76, 743–750.
Fischedick, J.T., Hazekamp, A., Erkelens, T., Choi,
Y.H., Verpoorte, R. (2010) Metabolic fingerprinting
of Cannabis sativa L., cannabinoids and terpenoids
for chemotaxonomic and drug standardization
purposes, Phytochemistry, 78, 2058–2073.
Knight, G., Hansen, S., Connor, M., Poulsen, H.,
McGovern, C., Stacey, J. (2010) The results of an
experimental indoor hydroponic Cannabis growing
study, using the ‘Screen of Green’ (ScrOG)
method—Yield, tetrahydrocannabinol (THC) and
DNA analysis, Forensic Science International, 202,
36–44.
Lata, H., Chandra, S., Khan, I.A., ElSohly, M.A.
(2010) High Frequency Plant Regeneration from
Leaf Derived Callus of High Δ9-
Tetrahydrocannabinol Yielding Cannabis sativa L.,
Planta Medica, 76, 1629–1633.
Mehmedic, Z., Chandra, S., Slade, D., Denham, H.,
Foster, S., Patel, A.S., Ross, S.A., Khan, I.A.,
ElSohly, M.A. (2010) Potency Trends of Δ9-THC
and Other Cannabinoids in Confiscated Cannabis
Preparations from 1993 to 2008*, Journal of
Forensic Sciences, 55, 1209–1217.
Hazekamp, A., Fischedick, J.T. (2012) Cannabis -
from cultivar to chemovar, Drug Testing and
Analysis, 4, 660–667.
Lata, H., Chandra, S., Techen, N., Khan, I.A.,
ElSohly, M.A. (2011) Molecular analysis of genetic
fidelity in Cannabis sativa L. plants grown from
synthetic (encapsulated) seeds following in vitro
storage, Biotechnology Letters, 33, 2503–2508.
Tipparat, P., Natakankitkul, S., Chamnivikaipong,
P., Chutiwat, S. (2012) Characteristics of
cannabinoids composition of Cannabis plants grown
in Northern Thailand and its forensic application,
Forensic Science International, 215, 164–170.
Happyana, N., Agnolet, S., Muntendam, R., Van
Dam, A., Schneider, B., Kayser, O. (2013) Analysis
of cannabinoids in laser-microdissected trichomes of
medicinal Cannabis sativa using LCMS and
cryogenic NMR, Phytochemistry, 87, 51–59.
Ameur, S., Haddou, B., Derriche, Z., Canselier, J.P.,
Gourdon, C. (2013) Cloud point extraction of Δ9-
tetrahydrocannabinol from cannabis resin,
Analytical and Bioanalytical Chemistry, 405, 3117–
3123.
Aizpurua-Olaizola, O., Omar, J., Navarro, P.,
Olivares, M., Etxebarria, N., Usobiaga, A. (2014)
Identification and quantification of cannabinoids in
Cannabis sativa L. plants by high performance
liquid chromatography-mass spectrometry,
Analytical and Bioanalytical Chemistry, 406, 7549–
7560.
Peschel, W., Politi, M. (2015) 1H NMR and
HPLC/DAD for Cannabis sativa L. chemotype
distinction, extract profiling and specification,
Talanta, 140, 150–165.
Deimler, R.E., Razunguzwa, T.T., Reschke, B.R.,
Walsh, C.M., Powell M.J., Jackson, G.P. (2014)
Direct analysis of drugs in forensic applications
using laser ablation electrospray ionization-tandem
mass spectrometry (LAESI-MS/MS), Analytical
Methods, 6, 4810–4817.
Eiras, M.M., De Oliveira, D.N., Ferreira, M.S.,
Benassi, M., Cazenave, S.O.S., Catharino, R.R.
(2014) Fast fingerprinting of cannabinoid markers
by laser desorption ionization using silica plate
extraction, Analytical Methods, 6, 1350–1352.
Nascimento, I.R., Costa, H.B., Souza, L.M.,
Soprani, L.C., Merlo, B.B., Romão, W. (2015)
Chemical identification of cannabinoids in street
marijuana samples using electrospray ionization FTICR
mass spectrometry, Analytical Methods, 7,
1415–1424.
Srivastava, A., VK, Y. (2013) Microscopical and
Chemical Study of Cannabis sativa, Forensic
Research, 5, 1–6.
Balbino, M.A., De Oliveira, L.S., Eleoterio, I.C.,
Oiye, E.N., Ribeiro, M.F.M., McCord, B.R., Ipolito,
A.J., De Oliveira, M.F. (2016) The Application of
Voltammetric Analysis of Δ9-THC for the
Reduction of False Positive Results in the Analysis
of Suspected Marijuana Plant Matter, Journal of
Forensic Sciences, 1–7.
Tanaka, H. (2011) Immunochemical Approach
Using Monoclonal Antibody against Δ9-
Tetrahydrocannabinolic Acid (THCA) to Discern
Cannabis Plants and to Investigate New Drug
Candidates, Current Drug Discovery Technologies,
8, 3–15.
Lata, H., Chandra, S., Techen, N. Khan, I.A.,
ElSohly, M.A. (2010) Assessment of the Genetic
Stability of Micropropagated Plants of Cannabis
sativa by ISSR Markers, Planta Medica, 76, 97–
100.
Allgeier, L., Hemenway, J., Shirley, N., LaNier, T.,
Coyle, H.M. (2011) Field Testing of Collection
Cards for Cannabis sativa Samples With a Single
Hexanucleotide DNA Marker, Journal of Forensic
Sciences, 56, 1245–1249.
Sutipatanasomboon, A., Panvisavas, N. (2011)
Discrimination of ‘fiber-type’ and ‘drug-type’
Cannabis sativa L.by fluorescent duplex PCR,
Forensic Science International: Genetics
Supplement Series 3, e522–e523.
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