Development and validation of analytical HPLC method for quantification of curcumin in soybean oil nanocapsules

Taiane Medeiro Ciocheta; Taiz Martins da Silva; Gabriel Pedroso Viçozzi; Jéssica Brandão Reolon; Eduardo André Bender; Letícia Marques Colomé

doi:10.22456/2527-2616.150106

Authors

Taiane Medeiro Ciocheta Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Pampa https://orcid.org/0009-0002-1137-4350
Taiz Martins da Silva Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Pampa https://orcid.org/0000-0002-4904-4640
Gabriel Pedroso Viçozzi Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Pampa https://orcid.org/0000-0002-4233-0206
Jéssica Brandão Reolon Departamento de Farmácia - Universidade Estadual do Centro-Oeste https://orcid.org/0000-0001-7388-8256
Eduardo André Bender Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Pampa https://orcid.org/0000-0001-5790-0649
Letícia Marques Colomé Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Pampa https://orcid.org/0000-0003-3275-1725

DOI:

https://doi.org/10.22456/2527-2616.150106

Keywords:

Curcumin, Soybean oil, HPLC, nanocapsules, Analytical validation

Abstract

This study aimed to develop and validate an analytical methodology for quantifying curcumin in soybean oil nanocapsules. The chromatography separation used a C18 column (Phenomenex, 4.6 x 150mm, 5μm) and a ternary mixture of acetonitrile, acidified water (pH 3.0), and methanol (53:42:5). The flow rate used was 1 mL.min^-1, and curcumin in the samples was detected at 425 nm. The chromatographic method proved specific for curcumin quantification and linear (correlation coefficient 0.9998). The limits of detection and quantification for the curcumin were 0.2575 µg.mL^-1 and 0.7804 µg.mL^-1, indicating the sensitivity of the analytical method. The repeatability and intermediate precision tests showed that the process is accurate (SRD less than 2%). In addition, the accuracy test showed values close to 100% for recovery of the bioactive. Thus, a new analytical method for quantifying curcumin in soybean oil nanocapsules may be used to analyze bioactive content.

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Author Biography

Eduardo André Bender, Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal do Pampa

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