A novel sensitive colorimetric determination of catecholamine drug in dosage form via oxidative coupling reaction with MBTH and potassium ferricyanide
DOI:
https://doi.org/10.22456/2527-2616.113058Keywords:
Methyldopa, MBTH, colorimetry, pharmaceutical dosage formsAbstract
A new and direct colorimetric method has been established for the determination of catecholamine (methyldopa, MD) in both pure form and in pharmaceutical formulations. The method is based on the oxidative coupling reaction of MD with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) and potassium ferricyanide at pH 10.4 in aqueous medium to form an orange product that has a maximum absorption at 460 nm. Beer's law plot showed good correlation in the concentration range of 1.0−56.0 µg mL-1, with detection limit of 0.31 µg mL-1. Molar absorptivity for the above method was found to be 6.56×103 L mol-1 cm-1. All the measurements were carried out at 25 ± 1.0 °C, the formation constant (logKf) value of colored species is 9.48 and the standard free energy (DG‡) is − 54.09 KJ mol-1. This method was applied successfully to determination of MD in tablets and the results were compared with the USP method. Common excipients used as additives in tablets do not interfere in the proposed method. The method is accurate, precise and highly reproducible, while being simple, cheap and less time consuming and hence can be suitably applied for routine analysis of MD in bulk and dosage forms.
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References
Hoffman BB. Catecholamines, sympathomimetic drugs, and adrenergic receptor antagonists. In: Hardman JG, Limbird LE, Molinoff PB, Ruddon RW, Gilman AG. (editors). Goodman & Gilman’s the pharmacological basis of therapeutics. New York, USA: Mac Graw-Hill, 2005, pp.163-165.
The United States Pharmacopoeia, 24th ed.; The United States Pharmacopoeial Convention. Rockville, Maryland, USA, 2000.
Amin D. Titrimetric determination of catecholamines and related compounds via bromine oxidation and substitution. Analyst 1986; 111(2): 255-257.
Salem FB. Titrimetric and spectrophotometric determination of catecholamines. Anal. Lett. 1993; 26(9): 1959-1966.
Antunes RS, Thomaz DV, Garcia LF, de Souza Gil E, Somerset VS et al. Determination of methyldopa and paracetamol in pharmaceutical samples by a low cost genipa americana L. polyphenol oxidase based biosensor. Advanced Pharmaceutical Bulletin 2019; 9(3): 416-422.
Zaimbashi R, Beitollahi H, Torkzadeh-Mahani M. Simultaneous electrochemical sensing of methyldopa and hydrochlorothiazide using a novel ZNO/AL2O3 nanocomposite modified screen printed electrode. Anal. Bioanal. Electrochem. 2017; 9(8): 1008-1020.
Baytak AK, Duzmen S, Teker T, Aslanoglu M. A novel modified electrode based on terbium oxide and carbon nanotubes for the simultaneous determination of methyldopa and paracetamol. Anal. Meth. 2016; 8(23): 4711-4719.
Gholivand MB, Amiri M. Highly sensitive and selective determination methyldopa in the presence of ascorbic acid using OPPy/TY/Au modified electrode. J. Electroanal. Chem. 2013; 694: 56–60.
Afsaneh L, Sanati, Faridbod F. Electrochemical determination of methyldopa by graphene quantum dot/1-butyl-3-methylimidazolium hexafluoro phosphate nanocomposite electrode. Int. J. Electrochem. Sci. 2017; 12: 7997–8005.
Badawy SS, Issa YM, Tag-Eldin AS. Potentiometric determination of L-dopa, carbidopa, methyldopa and aspartame using a new trinitrobenzenesulfonateselective electrode. Electroanalysis (N.Y.), 1996; 8(11): 1060-1064.
Athanasiou-Malaki EM, Koupparis MA. Indirect potentiometric determination of α-amino-acids with a copper-selective electrode and determination of dopa and methyldopa in pharmaceutical preparations. Anal. Chim. Acta 1984; 161: 349-353.
He SH, Li CY, Tian KJ. Study on the chemiluminescence determination of methyldopa with ferricyanide and dichlorofluorescein. Fenxi Kexue Xuebao 2006; 22(6): 707-709.
Chaichi MJ, Khajvand T, Mehrzad J, Asghari S, Qandalee M. Indirect chemiluminescence-based determination of catecholamines in pharmaceutical formulations by furandicarboxylate derivative as a novel blue fluorescer in peroxyoxalate-H2O2 System. Anal. Sci. 2013; 29(8): 815-821.
Garrido EM, Lima JL, Delerue-Matos C. Flow injection amperometric determination of L-dopa, epinephrine or dopamine in pharmaceutical preparations. J. Pharm. Biomed. Anal. 1997; 15(6): 845-849.
Wang C, Wang Z, Han DD, Hu YX, Zhao J et al. Simultaneous determination of levodopa and methyldopa in human serum by capillary electrophoresis. Sepu 2006; 24(4): 389-391.
Zecevic M, Zivanovic L, Agatonovic-Kustrin S, Minic D. The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. J. Pharm. Biomed. Anal. 2001; 24(5-6): 1019-1025.
Li SF,Wu HL, Yu YJ, Li YN, Nie JF et al. Quantitative analysis of levodopa, carbidopa and methyldopa in human plasma samples using HPLC-DAD combined with second-order calibration based on alternating trilinear decomposition algorithm. Talanta 2010; 81: 805–812.
Bahrami G, Kiani A, Mirzaeei S. A rapid high performance liquid chromatographic determination of methyldopa in human serum with fluorescence detection and alumina extraction: Application to a bioequivalence study. J. Chromatogr. B 2006; 832(2): 197-201.
Karimi M, Carl JL, Loftin S, Perlmutter JS. Modified high-performance liquid chromatography with electrochemical detection method for plasma measurment of levodopa, 3-O-methyldopa, dopamine, carbidopa, and 3,4-dihydroxyphenyl acetic acid. J. Chromatogr. B 2006, 836(1-2), 120-123.
Oliveira CH, Barrientos-Astigarraga RE, Sucupira M, Graudenz GS, Muscara MN et al. Quantification of methyldopa in human plasma by high-performance liquid chromatography-electrospray tandem mass spectrometry. Application to a bioequivalence study. J. Chromatogr. B 2002; 768(2): 341-348.
Talebpour Z, Haghgoo S, Shamsipur M. Proton nuclear magnetic resonance spectroscopy analysis for simultaneous determination of levodopa, carbidopa and methyldopa in human serum and pharmaceutical formulations. Anal. Chim. Acta 2004; 506(1): 97-104.
Davidson AG. Difference spectrophotometric assay of 1,2-diphenolic drugs in pharmaceutical formulations. II. Germanium dioxide reagent. J. Pharm. Sci. 1984; 73(11): 1582-1584.
Hamzah MJ, Shakir IMA. Flow injection photometric assay for determination of methyl dopa using hydrogel approch as a host for the sodium periodate solution. Int. J. Pharm. Sci. Rev. Res. 2016; 41(1): 247-251.
Ribeiro PRS, Gomes Neto JA, Pezza L, Pezza HR. Flow-injection spectrophotometric determination of methyldopa in pharmaceutical formulations. Talanta 2005; 67(1): 240–244.
Abood NK, Hassan MJM, Al-Da'amy MA. Determination of phenolic drugs and their formulations via various analytical methods. Res. J. Chem. Envir. 2019; 23(1): 188-199.
Abood NK, Hassan MJM, Al-Da'amy MA. Spectrophotometric determination methyldopa and salbutamol by oxidative coupling, cloud point and flow injection in pharmaceutical formulations. Int. J. Drug Deliv. Technol. 2019; 9(2): 182-192.
Tabrizi AB, Bahrami F, Badrouj H. A very Simple and sensitive spectrofluorimetric method based on the oxidation with cerium (IV) for the determination of four different drugs in their pharmaceutical formulations. Pharm. Sci. 2017; 23: 50-58.
Salem FB. Spectrophotometric and fluorimetric determination of catecholamines. Anal. Lett. 1993; 26(2): 281-294.
Kaushik RD, Yadav R, Sushma, Manila, Singh J. Development and validation of kineticspectrophotometric method for determination of methyldopa in aqueous formulations and tablets. Der Pharma Chemica 2014; 6(4): 102-108.
Tubino M, Batista DCDV, Rodrigues JAR. Kinetic method for the determination of α-methyldopa in pharmaceutical preparations: analytical procedure and reaction mechanism considerations. Anal. Lett. 2006; 39(1-3): 327-339.
Andrabi SMA, Aziz S, Najam R. Kinetic spectrophotometric determination of an important pharmaceutical compound, α-Methyldopa. J. Indian Chem. Soci. 2012; 89(2): 247-251.
Zakhari NA, Salem FB, Rizk MS. Spectrophotometric determination of catecholamines using chloranilic acid. Farmaco, Edizione Pratica 1987; 42(4): 103-109.
Upadhyay K, Asthana A, Tamrakar RK. Extractive spectrophotometric determination of -methyldopa in bulk dosage and in its formulations. Res. on Chem. Intermediates 2015; 41(8): 5521–5528.
Sane RT, Bhounsule GJ, Sawant SV. Colorimetric determination of levodopa and methyldopa from formulations. Indian Drugs 1987; 24(4): 207-209.
Emmanuel J, Shetty AR. Estimation of methyldopa and its dosage forms. Indian Drugs 1984; 21(9): 393-394.
Issopoulos PB. High-sensitivity spectrophotometric determination of trace amounts of levodopa, carbidopa and methyldopa. Fresenius' J. Anal. Chem. 1990; 336(2): 124-128.
Aman T, Khan IU, Aslam N, Ahmad I. Spectrophotometric determination of methyldopa in pure and pharmaceutical preparations. Anal. Lett. 1998; 31(6): 1007-1020.
Nagaraja P, Murthy KCS, Rangappa KS, Gowda NMM. Spectrophotometric methods for the determination of certain catecholamine derivatives in pharmaceutical preparations. Talanta 1998; 46(1): 39-44.
Nagaraja P, Vasantha RA, Sunitha KR. A sensitive and selective spectrophotometric estimation of catechol derivatives in pharmaceutical preparations Talanta 2001; 55(6): 1039-1046.
Afkhami A, Khatami HA. Determination of some catecholamines based on their reaction with periodate. Zhurnal Analiticheskoi Khimii (Russian J. Anal. Chem.) 2003; 58(2): 157-160.
Nagaralli B.S., Seetharamappa J., Melwanki M.B., Ramesh K.C., Keshavayya J., Spectrophotometric investigations of the assay of physiologically active catecholamines in pharmaceutical formulations, J. AOAC Int. 2002, 85(6), 1288-1292.
Nagaraja P, Vasantha RA, Sunitha KR. A new sensitive and selective spectrophotometric method for the determination of catechol derivatives and its pharmaceutical preparations. J. Pharm. Biomed. Anal. 2001; 25(3-4): 417-424.
Helaleh MIH, Rahman N, Abu-Nameh ESM. Use of cerium(IV) nitrate in the spectrophotometric determination of levodopa and methyldopa in the pure form and pharmaceutical preparations. Anal. Sci. 1997; 13(6): 1007-1010.
Al Samarrai KF, Al Samarrai ET, Al Samarrai BA. Spectrophotometric determination of methyldopa in pharmaceutical preparations via ion pair formation. Int. J. Res. Pharm. Sci. 2019; 10(2): 1367-1371.
da Silva Ribeiro PR, Duarte RM. Development and validation of a simple spectrophotometric method for the determination of methyldopa in both bulk and marketed dosage formulations. Braz. J. Pharm. Sci. 2014; 50(3): 573-582.
Matos OR, da Silva FC, da Silva Ribeiro PR. A new, simple and sensitive analytical method for determination of methyldopa in pharmaceutical formulations using the 2,2-diphenyil-picrylhydrazyl. Latin Amer. J. Pharm. 2012; 31(2): 190-194.
Gadkariem EA,, Ibrahim KEE, Kamil NAA, Haga MEM, El-Obeid HA. A new spectrophotometric method for the determination of methyldopa. Saudi Pharm. J. 2009; 17: 289– 293.
Basyoni Salem F. Spectrophotometric determination of catecholamines. J. Pharm. Belgique 1985; 40(3): 185- 190.
Rao GR, Raghuveer S, Khadgapathi P. Spectrophotometric determination of methyldopa and its dosage forms using 3-methylbenzothiazolin-2-one hydrazone hydrochloride. Indian Drugs 1985; 22(11): 597-599.
Rose J. Advanced physico-chemical experiments, Pittman, London, p. 67, 1964.
ICH, Q2 (R1). Validation of analytical procedures: text and methodology, Int. Conf. harm. Geneva; 2005.
Miller JN, Miller JC. Statistics and Chemometrics for Analytical Chemistry. London, UK: Chapman & Hall/CRC, 2005.
Long GL, Winefordner JD. Limit of detection. A closer look at the IUPAC definition. Anal. Chem. 1983; 55(7): 712A–721A.
Ashour S, Bayram R. Sensitive extractional colorimetric analysis of fexofenadine hydrochloride and irbesartan bases through acid-dye complexation using naphthol blue black in pure form and pharmaceuticals. Modern Chem. 2017; 5(6): 93–100.
Martin AN, Swarbrick J, Cammarata A. Physical Pharmacy. Lee and Febiger (editors). Philadelphia, USA: 1983, pp. 344–346.
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