Abnormal crosslink formation in root dentin collagen in type 2 Diabetes Mellitus
DOI:
https://doi.org/10.22456/2177-0018.144594Keywords:
Collagen, Dentin, Diabetes Mellitus, HyperglycemiaAbstract
Aim: To study root dentin collagen in individuals with diabetes, searching for possible chemical alterations due to reactions caused by hyperglycemia. Materials and Methods: Six extracted teeth roots, three from individuals with type 2 Diabetes Mellitus and three normoglycemic controls, were prepared for Fourier transform infrared spectroscopy with Attenuated Total Reflectance (FTIR – ATR) examination, generating spectra of three points on the internal face and three on the external face, totaling 36 spectroscopies. Results were analyzed by Principal Component Analysis (PCA). Scanning electron microscopy (SEM) was also performed to characterize the surfaces. Results: There was a distinction between some peaks (amides, carbonate and phosphate), which may suggest different molecular bonds (abnormal crosslinks) resulting from accumulation of AGEs due to hyperglycemia. However, it was not possible to separate the spectrometry patterns by control vs. diabetic group through the principal components (PCs) generated from infrared spectra. There was a tendency, in one of the comparisons between PCs, for separation of the external diabetic group. SEM images illustrated dentinal tubules in samples. Discussion: The tendency for separation of the external diabetic group suggests some alteration in the chemical composition of the dental tissue in this condition, which may be associated with changes in amide, carbonate and phosphate peaks, and requires further studies. Conclusion: Despite some altered peaks, it was not possible yet to conclude that there is formation of abnormal crosslinks in root dentin in type 2 DM, since spectrometry pattern did not differentiate control and diabetic samples.
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Copyright (c) 2026 Mariana Carreiro Costa de Lima, Isabela Monici Silva, Bruna Leis Endres, Sebastião William da Silva, Maria do Carmo Machado Guimarães, Naile Dame-Teixeira
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