The effect of pH on the simultaneous determination of Sulfamethoxazole and Trimethoprim by Ultraviolet Spectrophotometry and Multivariate Calibration
DOI:
https://doi.org/10.22456/2527-2616.79218Keywords:
chemometrics, sulphamethoxazole, trimethoprim, ultravioletAbstract
Analytical techniques based on Ultraviolet (UV) spectrophotometry are widely used in pharmaceutical analysis, because they are simple and inexpensive. The choice of pH is critical in the development of univariate methods for pharmaceutical quantitation by UV spectrophotometry since changes may modify the absorption spectrum profile. Similar to univariate methods by UV spectrophotometry changes in pH may influence the predictive ability of multivariate models, affecting the resultant analytical performance. We report herein on the influence of pH on the simultaneous determination of sulfamethoxazole (SMZ) and trimethoprim (TMP) in tablets using UV spectrophotometry and multivariate calibration. Data were recorded using a UV spectrophotometer in the wavelength range of 200 to 350 nm. The experimental matrix was constructed using 36 synthetic samples of SMZ-TMP mixtures. The concentration ranges used for the investigation were 14.0 to 26.0 mg L-1 for SMZ and 2.8 to 5.2 mg L-1 for TMP. The Partial Least Squares (PLS) regression models were generated with full-spectrum and multiple pH levels. At pH 4.3, lower values of relative standard error of prediction (RSEP %) for SMZ (1.83) and TMP (1.13) were obtained. The PLS model at pH 4.3 was used for the quantification of real samples (tablets obtained from 13 different manufacturers) and the results were compared with conventional procedures using high performance liquid chromatography (HPLC).
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References
Brunton L, Chabner B, Knollman B. Goodman and Gilman’s The Pharmacological Basis of Therapeutics. Twelfith e. MacGraw-Hill; 2011.
ANVISA. Farmacopeia Brasileira. 5°. Brasília; 2010.
United States Pharmacopoeial Convention. USP33-NF28. 33rd ed. Rockville: United States Pharmacopoeial Convention; 2010.
Adegoke OA, Babalola CP, Kotila OA, Obuebhor O. Simultaneous spectrophotometric determination of trimethoprim and sulphamethoxazole following charge-transfer complexation with chloranilic acid. Arab J Chem [Internet]. King Saud University; 2014; Available from: http://dx.doi.org/10.1016/j.arabjc.2014. 05.022
Medina JR, Miranda M, Hurtado M, Dominguéz-Ramírez AM, Ruiz-Segura JC. Simultaneous determination of trimethoprim and sulphamethoxazole in immediate-release oral dosage forms by first-order derivative spectroscopy: application to dissolution studies. Int J Pharm Pharm Sci. 2013;5(4):505–10.
6. López-Martinez L, López-de-Alba PL, De-Léon-Rodriguez LM, YepezMurrieta M de L. Simultaneous determination of binary mixtures of trimethoprim and sulfamethoxazole or sulphamethoxypyridazine by the bivariate calibration spectrophotometric method. J Pharm Biomed Anal. 2002;30:77–85. DOI: https://doi.org/10.1016/S0731-7085(02)00133-4
7. Goodarzi M, Shahbazikhah P, Sohrabi MR, Fathabadi M, Nouri SH. Direct orthogonal signal corretion-partial leas squares for simultaneous spectrophotometric determination of sulphamethoxazole and trimethoprim in pharmaceutical formulation and synthetic samples. J Chil Chem Soc. 2009;54(3):309–13. DOI: https://doi.org/10.4067/S0717-97072009000300022
Silva FEB, Ferrão MF, Parisotto G, Müller EI, Flores EMM. Simultaneous determination of sulphamethoxazole and trimethoprim in powder mixtures by attenuated total reflection-Fourier transform infrared and multivariate calibration. J Pharm Biomed Anal. 2009;49(3):800–5. DOI: https://doi.org/10.1016/j.jpba.2008.12.011
Silva FEB, Ziech C, Dias YP. Quantitative Determination of Sulphamethoxazole and Trimethoprim in Powder Mixtures Using Raman Spectroscopy and PLS. Lat Am J Pharm. 2011;30(6):1216–20.
Alves LDS, Rolim LA, Fontes AF, Rolim-Neto PJ, Soares MFDLR, Sobrinho JLS. Desenvolvimento de método analítico para quantificação do efavirens por espectrofotomeria no UVVIS. Quim Nova. 2010;33(9):1967–72. DOI: https://doi.org/10.1590/S0100-40422010000900026
Bonfilio R, Araújo MB De, Salgado HRN. Development and validation of an UV-derivative spectrophotometric method for determination of glimepiride in tablets. J Braz Chem Soc. 2011;22(2):292–9. DOI: https://doi.org/10.1590/S0103-50532011000200015
Polonini HC, Santos FC dos, Vaz UP, Brandão MAF. Desenvolvimento e validação de método analítico para determinação do teor de sinvastatina em cápsulas magistrais. Quim Nova. 2011;34(3):516–9. DOI: https://doi.org/10.1590/S0100-40422011000300026
Borba PAA, Riekes MK, Pereira RN, Stulzer HK, Vecchia DD. Desenvolvimento e validação de um método analitico por espectrometria UV para quantificação de carvedilol. Quim Nova. 2013;36(4):582–6. DOI: https://doi.org/10.1590/S0100-40422013000400017
Mendez ASL, Cassol JPE, Camargo VB De, Malesuik MD, Garcia C V. Quantitative Determination of Paliperidone in OROS ® Tablets by Deriva- tive Spectrophotometric Method – Application in Extraction and Comparison to HPLC. Curr Anal Chem. 2014;10(1):158–65. DOI: https://doi.org/10.2174/1573411011410010014
Maggio RM, Rivero M a., Kaufman TS. Simultaneous acquisition of the dissolution curves of two active ingredients in a binary pharmaceutical association, employing an on-line circulation system and chemometricsassistance. J Pharm Biomed Anal [Internet]. Elsevier B.V.; 2013;72:51–8. Available from: http://dx.doi.org/10.1016/j.jpba.2012.09 .022 DOI: https://doi.org/10.1016/j.jpba.2012.09.022
Roggo Y, Chalus P, Maurer L, LemaMartinez C, Edmond A, Jent N. A review of near infrared spectroscopy and chemometrics in pharmaceutical technologies. J Pharm Biomed Anal. 2007;44:683–700. DOI: https://doi.org/10.1016/j.jpba.2007.03.023
Hegazy MA-M, Eissa MS, El-Sattar OIA, El-Kawy MMA. Determination of a novel ACE inhibitor in the presence of alkaline and oxidative degradation products using smart spectrophotometric and chemometric methods. J Pharm Anal. 2014;4(2):132–43. DOI: https://doi.org/10.1016/j.jpha.2013.09.006
Piantavini MS, Pontes FLD, Weiss LX, Sena MM, Pontarolo R. Comparison between Ultraviolet and Infrared Spectroscopies for the Simultaneous Multivariate Determination of Pyrantel and Praziquantel. J Brazilian Chem Soc. 2015;26(7):1387–95. DOI: https://doi.org/10.5935/0103-5053.20150107
Cordeiro GA, Peralta-Zamora P, Nagata N, Pontarollo R. Determinação de misturas de sulfametoxazol e trimetoprima por espectroscopia eletrônica multivariada. Quim Nova. 2008;31(2):254–60. DOI: https://doi.org/10.1590/S0100-40422008000200012
Dohoda D, Tsinman K, Tsinman O, Wang H, Tam KY. Spectrophotometric pKa determination of ionizable pharmaceuticals: Resolution of molecules with weak pH-dependent spectral shift. J Pharm Biomed Anal. Elsevier B.V.; 2015;114:88–96. DOI: https://doi.org/10.1016/j.jpba.2015.05.009
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