High frequency of potentially pathogenic yeast species in goat’s raw milk and creamed cheese in Southern Brazil
Keywords:Identificação de leveduras, Leite de cabra, Queijo de cabra, HMA, Candida spp.
There are few reports concerning isolation, counting and identification of yeasts in goat´s raw milk and derivates. The objective of this study was to evaluate the diversity of yeasts found in raw goat milk and goat creamed cheese collected in a Metropolitan area in Porto Alegre - Brazil. A simplified HMA (Heteroduplex Mobility Assay) of the 26S rDNA D1/D2 region was developed in order to rapidly confirm the identification of the isolates belonging to potentially pathogenic species. Yeasts were isolated from 59% of the samples. Fifty six strains were isolated and identified in the genera
Bullera, Candida, Cryptococcus, Debaryomyces, Dekkera, Pichia, Rhodotorula, Sporodiobolus, Trichosporon, Yarrowia and Zygoascus. The average yeast count in raw milk was superior to 2 log UFC.mL-1, while cheese count was superior to 3 log UFC.g-1. Lipolytic activity was present in almost 92% of the isolates, while only 14% had proteolytic activity. Twelve potentially pathogenic ascomycetic isolates were identified by the conventional yeast identification methodology, and correspond to the species Candida parapsilosis, Candida tropicalis and Pichia guilliermondii. All of them had their identities confirmed by the simplified HMA assay. None of the isolates belonging to potentially pathogenic species were resistant to the antifungal agents tested. More studies are necessary to evaluate the real significance of the isolation of these clinically relevant yeasts.
How to Cite
This journal provides open access to all of its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Such access is associated with increased readership and increased citation of an author's work. For more information on this approach, see the Public Knowledge Project and Directory of Open Access Journals.
We define open access journals as journals that use a funding model that does not charge readers or their institutions for access. From the BOAI definition of "open access" we take the right of users to "read, download, copy, distribute, print, search, or link to the full texts of these articles" as mandatory for a journal to be included in the directory.
La Red y Portal Iberoamericano de Revistas Científicas de Veterinaria de Libre Acceso reúne a las principales publicaciones científicas editadas en España, Portugal, Latino América y otros países del ámbito latino