Endophytic microorganisms and plant tissue culture: breaking paradigms

Abstract

Plant tissue culture has been an important tool widely used for various purposes. Within this context, the term axenic plant has been taken as an ideal to be achieved in this technique, which the search based on methods that effectively eliminate any microorganisms that may eventually grow into the culture medium. However, it is seen that the conception of contamination in tissue culture should be reviewed as well as axenic plants, because several studies have shown that even in asymptomatic microplants considered axenic, there is an ubiquitous and that the endophytic community mostly plays a fundamental role in the establishment, development and acclimatization of cultures, therefore, beneficial microorganisms that should be preserved in vitro.