Extracellular extract of Bacillus sp. halotolerant bacterium is cytotoxic to 786.0 human renal adenocarcinoma cell line

Abstract

The halotolerant microorganisms are considered as a promising source of bioproducts with potential interest for many industrial sectors. The potential of biotechnological applications of these microorganisms highlights the importance of research for biologically active biomolecules. In this context, this study aimed to evaluate the in vitro cytotoxicity of extracellular extract halotolerant bacterium Bacillus sp. in human renal cell carcinoma. The bacteria were obtained from saline Saint Camillus (S 05 ° 03 947 ', N 37 ° 16,318, Natal, Rio Grande do Norte, Brazil) and isolated in BHI medium at 37 °C. Biochemical and morphological analyzes classified the species as halotolerant, supporting up to 13% NaCl. Extracellular extract was obtained by ethyl acetate immersion in culture medium after a 24 h incubation. Cytotoxicity was measured by MTT assay. Cells were cultured for 24 h (5x 10³ and 1x 10⁴ cells/well) with different concentrations of the extract. Concentrations of 75 and 100 μg/mL caused severe cytotoxic effects against the cell line 786.0. We conclude that the extracellular extract of Bacillus sp. resulted in severe cytotoxic activity on human kidney cancer cells and not cancerous cells.