In Vitro development of Dendrobium nobile Lindl. (Orchidaceae) in vessels of different volumes

Abstract

Commercial cultivation of Dendrobium nobile Lindl. is of great importance to the agribusiness floricultural world, but the slow development of the plant contributes to the high unit value of the plant in the market. The massal propagation through sowing seeds in vitro is an important method for propagation orchid commercial species. The type of the flasks and the amount of culture medium used are variables that have received little attention although they directly affect the superficial area of the medium-air culture interface, depth, volume of air over the culture medium, and the identification of the ideal vessels for each species can optimize the production. The present study aimed to evaluate the in vitro development of D. nobile seedlings in different size vessels, each containing the same volume of MS culture (30 mL). D nobile seeds were inoculated in flasks of 100 mL, 200 mL, and 400 mL and cultivated in BOD for 180 days. The results showed that the development of the D. nobile seedlings was promoted by the use of 100 mL-flasks, which could then be used for commercial propagation.