Evaluation of quality and safety parameters of DEET commercial repellents: photostability, penetration/permeation and eye irritation studies: Evaluation of quality and safety parameters of DEET commercial repellents

Juliana dos Santos; Rebeca Lino Lourenço; Priscila Rosa; Andréa Inês Horn Adams

doi:10.22456/2527-2616.124882

Authors

Juliana dos Santos Pharmaceutical Sciences Graduate Program - Federal University of Santa Maria
Rebeca Lino Lourenço Department of Industrial Pharmacy - Federal University of Santa Maria
Priscila Rosa Pharmaceutical Sciences Graduate Program - Federal University of Santa Maria
Andréa Inês Horn Adams Pharmaceutical Sciences Graduate Program; Department of Industrial Pharmacy - Federal University of Santa Maria

DOI:

https://doi.org/10.22456/2527-2616.124882

Keywords:

Penetration/permeation, HET-CAM, degradation kinetics, repellents, DEET

Abstract

N',N'-diethyl-m-toluamide (DEET) is the repellent most commonly used against mosquito vectors of diseases such as dengue, yellow fever and chikungunya. In Brazil, DEET is marketed in aerosol, solution, lotion and gel forms, at concentrations ranging from 6.65 to 25%. In this study, the kinetic degradation under UVC radiation was studied, as well as the penetration/permeation and the ocular irritant potential of DEET repellents in the form of solution, lotion and gel. The photostability study was conducted over 96h, and the DEET degradation kinetics under UVC radiation was fitted to the zero-order model for the three formulations; t90% values of 23.7 h, 17.0 h and 16.1 h were obtained for gel, lotion and solution forms, respectively. The in vitro skin penetration/permeation using the vertical Franz cell in pig skin showed that all the formulations penetrated/permeated the skin layers, at a higher rate when the lotion (p<0.05) was used, possibly due to its qualitative composition. The in vitro ocular irritant potential using the HET-CAM method indicated that one of the products evaluated was classified as "moderate irritant" and five as "severe irritant”. The set of data indicated the lower penetration/permeation of the solution form and reinforces the importance of being careful during application, to avoid accidental contact with the eyes.

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Author Biography

Juliana dos Santos, Pharmaceutical Sciences Graduate Program - Federal University of Santa Maria

Programa de Pós-Graduação em Ciências Farmacêuticas - Universidade Federal de Santa Maria, RS, Brasil

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Evaluation of quality and safety parameters of DEET commercial repellents: photostability, penetration/permeation and eye irritation studies