Prevalence and Molecular Characterization of Giardia duodenalis in Calves in Turkey
DOI:
https://doi.org/10.22456/1679-9216.80032Keywords:
calf, giardiosis, prevalence, molecular characterization.Abstract
Background: Giardia duodenalis (G. duodenalis) is an ubiquitous, flagellated intestinal protozoan with major public health significance worldwide. Limited data are available on the epidemiology of G. duodenalis in dairy cattle from Turkey. Determining the zoonotic potential of the Giardia infection requires molecular characterization. The aim of the present study was to investigate the prevalence and to molecularly characterize G. duodenalis in calves less than three months of age in Aydin, Aegean region of Turkey.
Materials, Methods & Results: The study was conducted on different dairy farms in the south-western part of the Turkey, Aegean Region, Aydin. A total of 198 Holstein Friesian calves less than three months of age, of both sexes were enrolled into the study. Faecal samples from each calf were collected manually from the rectum using a disposable latex glove. The consistency of collected samples was recorded as diarrhoeic or non-diarrhoeic. Diagnosis of G. duodenalis infection was made microscopically by detection of cysts in the faecal samples. One hundred and sixteen (58.5%) of the 198 faecal samples were diarrheic. Giardia cysts were found in 27 (23.28%) of the diarrheic samples and in 8 (9.76%) of nondiarrheic samples (P < 0.05). The overall prevalence of giardiosis in calves was determined as 17.67%. The prevalence of Giardia genotypes was identified by DNA sequence analysis of the beta-giardin gene for every PCR positive sample. The beta-giardin nested PCR assay was revealed assemblage A and sub-genotype A3 was detected in all of 35 samples (100%).
Discussion: The highest prevalence of Giardia infection in calves is reported at the age between 1 and 6 months, and the prevalence shows decreased rate from the age of 6 months. The present study was conducted in Aydin, a province of south-western Turkey in the Aegean Region, and the overall prevalence from a total of 198 dairy calves was 17.67%. The prevalence rate in calves with diarrhoea was higher and reached up to 23.28%, whereas it was 9.76% in non-diarrhoeic calves. A prevalence study with molecular characterization of G. duodenalis isolates in cattle has not yet been reported from Turkey. Molecular studies have shown that mostly assemblage E predominates in cattle, but recent studies denoted that assemblage A is increasingly being detected and might be more widespread than expected before. In the present study, Giardia positive samples identified with a beta-giardin nested PCR assay. The sub-genotype A3 was identified in all samples. The same sub-genotype was identified in human and dog samples from different countries. Furthermore, sub-genotype A3 was found in humans and dogs from Turkey. In this context, results of the present study suggested an important role of calves as potential reservoirs of human infections in Turkey. In conclusion, epidemiological data revealed that G. duodenalis infection is frequent in calves with diarrhoea in Aydin, Turkey. The presence of the potentially zoonotic sub-genotype A3 and high prevalence of Giardia infection in diarrheic calves indicated the importance of treatment and necessary preventative measures. Further studies in human and animal populations living in this region are warranted regarding the zoonotic epidemiology of Giardia duodenalis.
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Aloisio F., Filippini G., Antenucci P., Lepri E., Pezzotti G., Caccio S.M. & Pozio E. 2006. Severe weight loss in lambs infected with Giardia duodenalis assemblage B. Veterinary Parasitology. 142: 154-158.
Bajer A. 2008. Cryptosporidium and Giardia spp. infections in humans, animals and the environment in Poland. Parasitology Research. 104(1): 1-7.
Berrilli F., Di Cave D., De Liberato C., Franco A., Scaramozzino P. & Orecchia P. 2004. Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing. Veterinary Parasitology. 122(3): 193-199.
Caccio S.M. 2015. Giardiasis: A zoonotic infection or not? In: Sing A. (Ed). Zoonoses-Infections Affecting Humans and Animals. New-York: Springer, pp.821-848.
Cacció S.M., de Giacomo M. & Pozio E. 2002. Sequence analysis of the ′ giardin gene and development of a PCRRFLP assay to genotype Giardia duodenalis cysts from human faecal samples. International Journal of Parasitology. 32: 1023-1030.
Cardona G.A., de Lucio A., Bailo B., Cano L., de Fuentes I. & Carmena D. 2015. Unexpected finding of felinespecific Giardia duodenalis assemblage F and Cryptosporidium felis in asymptomatic adult cattle in Northern Spain. Veterinary Parasitology. 209(3-4): 258-263.
Castro-Hermida J.A., Almeida A., González-Warleta M., da Costa J.M.C., Rumbo-Lorenzo C. & Mezo M. 2007. Occurrence of Cryptosporidium parvum and Giardia duodenalis in healthy adult domestic ruminants. Parasitology Research. 101(5): 1443-1448.
Cicek C. & Sakru N. 2015. Genotyping of Giardia intestinalis isolates in the Thrace Region, Turkey. Mikrobiyoloji Bulteni. 49(4): 576-585.
Coklin T., Farber J., Parrington L. & Dixon B. 2007. Prevalence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in dairy cattle in Ontario, Canada. Veterinary Parasitology. 150: 297-305.
Covacin C., Aucoin D.P., Elliot A. & Thompson R.C.A. 2011. Genotypic characterisation of Giardia from domestic dogs in the USA. Veterinary Parasitology. 177(1): 28-32.
Degerli S., Celiksoz A., Kalkan K. & Ozcelik S. 2005. Prevalence of Cryptosporidium spp. and Giardia spp. in cows and calves in Sivas. Turkish Journal of Veterinary Animal Science. 29: 995-999.
Dixon B., Parrington L., Cook A., Pintar K., Pollari F., Kelton D. & Farber J. 2011. The potential for zoonotic transmission of Giardia duodenalis and Cryptosporidium spp. from beef and dairy cattle in Ontario, Canada. Veterinary Parasitology. 175: 20-26.
Ertug S., Ertabaklar H., Caliskan S., Malatyali E. & Bozdogan B. 2016. Genotyping of Giardia intestinalis strains isolated from humans in Aydin, Turkey. Mikrobiyoloji Bulteni. 50(1): 152-158.
Feng Y. & Xiao L. 2011. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clinics of Microbiology Review. 24(1): 110-140.
Feng Y., Ortega Y., Cama V., Terrel J. & Xiao L. 2008. High intragenotypic diversity of Giardia duodenalis in dairy cattle on three farms. Parasitology Research. 103: 87-92.
Geurden T., Claerebout E., Dursin L., Deflandre A., Bernay F., Kaltsatos V. & Vercruysse J. 2006. The efficacy of an oral treatment with paromomycin against an experimental infection with Giardia in calves. Veterinary Parasitology. 135(3-4): 241-247.
Geurden T., Geldhof P., Levecke B., Martens C., Berkvens D., Casaert S., Vercruysse J. & Claerebout E. 2008. Mixed Giardia duodenalis assemblage A and E infections in calves. International Journal of Parasitology. 38: 259-264.
Geurden T., Vercruysse J. & Claerebout E. 2010. Is Giardia a significant pathogen in production animals? Experimental Parasitology. 124(1): 98-106.
Gillhuber J., Pallant L., Ash A., Thompson R.A., Pfister K. & Scheuerle M.C. 2013. Molecular identification of zoonotic and livestock-specific Giardia-species in faecal samples of calves in Southern Germany. Parasite & Vectors. 6(1): 1.
Goz Y., Altuğ N., Yüksek N. & Özkan C. 2006. Parasites detected in neonatal and young calves with diarrhoea. Bulletin of the Veterinary Institute in Pulawy. 50: 345-248.
Gul A., Cicek M. & Kilinc O. 2008. Prevalence of Eimeria spp., Cryptosporidium spp. and Giardia spp. in calves in the Van province. Turkiye Parazitoloji Dergisi. 32(3): 202-204.
Gultekin M., Ural K., Aysul N., Ayan A., Balikci C. & Akyildiz G. 2017. Prevalence and molecular characterization of Giardia duodenalis in dogs in Aydin, Turkey. International Journal of Environmental Health Research. 27(3): 1-8.
Hamnes I.S., Gjerde B. & Robertson L. 2006. Prevalence of Giardia and Cryptosporidium in dairy calves in three areas of Norway. Veterinary Parasitology. 140(3): 204-216.
Hoar B.R., Paul R.R., Siembieda J., Maria das Gracas C.P. & Atwill E.R. 2009. Giardia duodenalis in feedlot cattle from the central and western United States. BMC Veterinary Research. 5(1): 1.
Jäger M., Gauly M., Bauer C., Failing K., Erhardt G. & Zahner H. 2005. Endoparasites in calves of beef cattle herds: management systems dependent and genetic influences. Veterinary Parasitology. 131(3):173-191.
Lalle M., Pozio E., Capelli G., Bruschi F., Crotti D. & Cacció S.M. 2005. Genetic heterogeneity at the ß-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. International Journal of Parasitology. 35: 207-213.
Langkjær R.B., Vigre H., Enemark H.L. & Maddox-Hyttel C. 2007. Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark. Parasitology. 134(3): 339-350.
Lebbad M,. Mattsson J.G., Christensson B., Ljungström B., Backhans A., Andersson J.O. & Svärd S.G. 2010. From mouse to moose: multilocus genotyping of Giardia isolates from various animal species. Veterinary Parasitology. 168(3-4): 231-239.
Lee S.H., VanBik D., Kim H.Y., Cho A., Kim J.W., Byun J.W., Oem J.K., Oh S.I. & Kwak D. 2016. Prevalence and molecular characterisation of Giardia duodenalis in calves with diarrhoea. Veterinary Record. 178(25): 633.
Mahdy A.K.M., Surin J., Wan K.L., Mohd-Adnan A., Al-Mekhlafi M.S.H. & Lim Y.A.L. 2009. Giardia intestinalis genotypes: Risk factors and correlation with clinical symptoms. Acta Tropica. 112: 67-70.
Mark-Carew M.P., Wade S.E., Chang Y.F., Schaaf S. & Mohammed H.O. 2012. Prevalence of Giardia duodenalis assemblages among dairy herds in the New York City Watershed. Veterinary Parasitology. 185: 151-157.
Mendonça C., Almeida A., Castro A., de Lurdes Delgado M., Soares S., da Costa J.M. & Canada N. 2007. Molecular characterization of Cryptosporidium and Giardia isolates from cattle from Portugal. Veterinary Parasitology. 147(1): 47-50.
Minetti C., Taweenan W., Hogg R., Featherstone C., Randle N., Latham S.M. & Wastling J.M. 2014. Occurrence and Diversity of Giardia duodenalis Assemblages in Livestock in the UK. Transboundary and Emerging Diseases. 61(6): 60-67.
O’Handley R.M., Cockwill C., McAllister T.A., Jelinski T.A., Morck D.W. & Olson M.E. 1999. Duration of naturally acquired giardiasis and cryptosporidiosis in dairy calves and their association with diarrhea. Journal of American Veterinary Medical Association. 214: 391-396.
O’Handley R.M., Olson M.E. & McAllister T.A. 1997. Efficacy of fenbendazole for treatment of giardiasis in calves. American Journal of Veterinary Research. 58: 384-348.
O’handley R.M., Olson M.E., Fraser D., Adams P. & Thompson R.C.A. 2000. Prevalence and genotypic characterisation of Giardia in dairy calves from Western Australia and Western Canada. Veterinary Parasitology. 90(3): 193-200.
O’Handley R.M. & Olson M.E. 2006. Giardiasis and cryptosporidiosis in ruminants. Veterinary Clinics of North America Food Animal Practise. 22(3): 623-643.
Olson M.E., O’Handley R.M., Ralston B.J., McAllister T.A. & Thompson R.C. 2004. Update on Cryptosporidium and Giardia infections in cattle. Trends in Parasitology. 20: 185-191.
Rosethal A., Coutelle O. & Craxton M. 1993. Large-scale production of DNA sequencing templates by microtitre format PCR. Nucleic Acids Research. 21: 173-174.
Sprong H., Cacciò S.M. & Van der Giessen J. 2009. Identification of zoonotic genotypes of Giardia duodenalis. PLOS Neglected Tropical Diseases. 3(12): 558.
Trout J.M., Santín M. & Fayer R. 2007. Prevalence of Giardia duodenalis genotypes in adult dairy cows. Veterinary Parasitology. 147: 205-209.
Wang H., Zhao G., Chen G., Jian F., Zhang S., Feng C. & Wang M. 2014. Multilocus genotyping of Giardia duodenalis in dairy cattle in Henan, China. PLoS One. 9(6): e100453.
Wegayehu T., Karim M.R., Erko B., Zhang L. & Tilahun G. 2016. Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia. Infection, Genetics and Evolution. 43: 281-288.
Wilson J.M. & Hankerson F.C. 2009. Evaluation of an inhouse rapid ELISA test for detection of Giardia in domestic sheep (Ovis aries). American Association for Laboratory Animal Science. 49(6): 809-813. 45 Xiao L. & Herd R.P. 1994. Infection patterns of Cryptosporidium and Giardia in calves. Veterinary Parasitology. 55(3): 257-262.
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