Production and evaluation of albino mutants of Sporothrix schenckii

Authors

  • Márcia de Oliveira Nobre UFPEL
  • Tatiana de Ávila Antunes UFPEL
  • Mário Carlos Araújo Meireles UFPEL
  • Laerte Ferreiro UFRGS

DOI:

https://doi.org/10.22456/1679-9216.16833

Keywords:

Sporothrix schenckii, Mutantes albinos, Sobrevivência, Viabilidade

Abstract

Melanin is an important factor intrinsically related to the virulence of the

 

Sporothrix schenckii. Mutant albino conidia are usually used as inoculants for the evaluation of the properties of melanin in experimental models for the study of sporotrichosis. The goals of this study were to analyze the presence of melanin in S. schenckii conidia, and to produce and evaluate mutant albino forms of S. Schenckii conidia. Four distinct isolates were cultured in PDA, supplemented or not with triciclazole, and in YEPD. A portion of the samples was exposed to 300 ergs/mm2 of UV radiation to generate mutants albino forms. Following the incubation, cultures were analyzed, CFU were determined, and fragments were removed for the ultrastructural analysis, to be cultured once more in YEPD e BHI. Albino colonies were exposed to visible light; all S. schenckii isolates produced melanin, and survival and viability were 66% and 96% for albino conidia. Melanin granules were visible in the cell wall of pigmented conidia, but not in albino conidia. All conidia reverted to both the pigmented and the yeast forms, and albino colonies photoreactivated. All isolates analyzed in this study generated DHN melanin. Moreover, despite the exposure to UV radiation (300 ergs/mm2) being lethal to a significant portion of the colonies, the viability and conversions to the pigmented and yeast forms in surviving colonies did not appear to be affected. Also, albino colonies photoreactivated upon exposure to visible light.

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Author Biographies

Márcia de Oliveira Nobre, UFPEL

U

Tatiana de Ávila Antunes, UFPEL

Universidade Federal de Pelotas, RS.

Mário Carlos Araújo Meireles, UFPEL

Universidade Federal de Pelotas, RS.

Laerte Ferreiro, UFRGS

Universidade Federal do Rio Grande do Sul, Faculdade de Veterinária, RS.

Published

2018-06-27

How to Cite

Nobre, M. de O., Antunes, T. de Ávila, Meireles, M. C. A., & Ferreiro, L. (2018). Production and evaluation of albino mutants of Sporothrix schenckii. Acta Scientiae Veterinariae, 32(2), 119–123. https://doi.org/10.22456/1679-9216.16833

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