Development of experimental sporothrichosis in a murine model with yeast and mycelial forms of Sporothrix schenkii

Authors

  • Márcia de Oliveira Nobre UFRGS
  • Tatiana de Ávila Antunes UFPEL
  • Izamara Aparecida de Oliveira UFRGS
  • Vanessa Berg UFRGS
  • Thomaz Lucia Jr. UFPEL
  • Cristina Gevehr Fernandes UFPEL
  • Mário Carlos Araújo Meireles UFPEL
  • Laerte Ferreiro UFRGS

DOI:

https://doi.org/10.22456/1679-9216.17160

Keywords:

Sporothrix schenkii, Esporotricose experimental, forma leveduriforme, forma filamentosa, modelo murino, camundongos

Abstract

Sporotrichosis is a subcutaneous disease that affects both men and a variety of animals caused by the dimorphic fungus

 

Sporothrix schenckii. The aims of this study were to observe differences in virulence between inocula containing the yeast (Y) or mycelial (M) forms, to compare the reactions induced by the two inocula injected in different anatomical sites, and to verify the possibility of horizontal transmission of the disease by contact between infected and healthy animals. All animals were observed during a period of nine weeks. Mice were inoculated subcutaneously in the paw pads and in the regions of the hip and shoulder joints. Another group of non-inoculated healthy mice was kept in direct contact with mice developing the subcutaneous lesions. Animals of groups Y and M developed lesions due to infections and clinical symptoms very characteristic of sporothrichosis. Lesions were more prominent and with longer duration when occurring in the pads than in any other site of inoculation. Non-inoculated mice remained healthy as it occurred to animals in the control group. The development of typical clinical symptoms for sporothrichosis in all inoculated groups suggests that no difference exists in virulence between the yeast and mycelial forms of the same isolated of S. schenckii. Moreover, the paw pads appear to be the site of choice for the inoculation for experimental sporotrichosis in the murine model. In addition, this study also showed that the contact with sick animals and contaminated materials is insufficient to transmit the infection to immune competent animals with intact skin.

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Author Biographies

Márcia de Oliveira Nobre, UFRGS

Tatiana de Ávila Antunes, UFPEL

 

 

PPG em Veterinária - UFPEL.

Izamara Aparecida de Oliveira, UFRGS

 

 

Setor de Micologia, Departamento de Patologia Clínica Veterinária, FAVET/UFRGS.

Vanessa Berg, UFRGS

 

 

Setor de Micologia, Departamento de Patologia Clínica Veterinária, FAVET/UFRGS.

Thomaz Lucia Jr., UFPEL

 

 

Departamento de Patologia Animal e FAVET/UFPEL.

Cristina Gevehr Fernandes, UFPEL

 

 

Departamento de Patologia Animal e FAVET/UFPEL.

Mário Carlos Araújo Meireles, UFPEL

 

 

Departamento de Veterinária Preventiva, FAVET/UFPEL.

Laerte Ferreiro, UFRGS

 

 

Programa de Pós-Graduação em Ciências Veterinárias (PPGCV) - UFRGS.

 

 

Setor de Micologia, Departamento de Patologia Clínica Veterinária, FAVET/UFRGS

Published

2018-06-27

How to Cite

Nobre, M. de O., Antunes, T. de Ávila, Oliveira, I. A. de, Berg, V., Lucia Jr., T., Fernandes, C. G., Meireles, M. C. A., & Ferreiro, L. (2018). Development of experimental sporothrichosis in a murine model with yeast and mycelial forms of Sporothrix schenkii. Acta Scientiae Veterinariae, 31(3), 161–166. https://doi.org/10.22456/1679-9216.17160

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