TY - JOUR AU - Barcellos, David Emilio Santos Neves de AU - Mathiesen, Michelle AU - Duhamel, Gerald PY - 2018/06/27 Y2 - 2024/03/28 TI - Survival of pathogenic intestinal Spirochetes kept in pure cultures and in pig feces at four different temperatures JF - Acta Scientiae Veterinariae JA - Acta Scientiae Vet. VL - 30 IS - 3 SE - DO - 10.22456/1679-9216.17224 UR - https://seer.ufrgs.br/index.php/ActaScientiaeVeterinariae/article/view/17224 SP - 151-157 AB - <span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;"><font face="Times New Roman" size="2"><font face="Times New Roman" size="2"><p align="left">Porcine colonic spirochetosis (PCS) caused by</p></font></font></span><font face="Times New Roman" size="2"><p align="left"> </p></font></span><p align="left"><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">has been identified as a contributing cause of diarrhea and reduced performance of growing pigs in all major swine producing countries. The current view that transmission of PCS occurs through contamination of the environment by acutely or persistently infected pigs is based on the assumption that the spirochetes remain viable in the environment. The purpose of this study was to compare the viability of </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">kept in pure culture or mixed with feces at four different temperatures over time with that of </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira hyodysenteriae</span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">. The results of the present study indicated that </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">survived significantly longer than </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira hyodysenteriae </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">in pure cultures held at 24°C and 37°C, and at all temperatures in spiked fecal materials. Pure cultures of </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">survived at least 63 days at -70°C, seven days at 4°C, 14 to 28 days at 24°C and seven to 28 days at 37°C. There was significant differences in the survival of the 2 species of spirochetes when mixed with feces. At -70°C, </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">and </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira hyodysenteriae </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">survived respectively an average of 21 and 3 days, and at 4°C 12,25 and 4,25 days. Viability was reduced to one to seven days at 24°C and one to three days at 37°C for </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">and < five days at 24°C and < one day at 37°C for </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira hyodysenteriae</span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">. Information on the survival of </span></span><em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">Brachyspira pilosicoli </span></span></em><span style="font-family: Times New Roman; font-size: x-small;"><span style="font-family: Times New Roman; font-size: x-small;">outside the pig’s body provides a basis to improve strategies for PCS control.</span></span></p> ER -