Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

Authors

  • José Carlos Ferreira-Silva Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Pábola Santos Nascimento Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Marcelo Tigre Moura Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Sarah Romini Lima Basto Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Marlon Vasconcelos Azevedo Equestre Clínica, Cirurgia e Reprodução Animal, Campina Grande, PB, Brazil.
  • Jorge Motta Rocha Universidade Federal do Rio Grande do Norte, Natal-RN, Brazil.
  • José Pompeu Santos Filho
  • Marcos Antonio Lemos Oliveira Laboratório de Biotécnicas Reprodutivas, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.

DOI:

https://doi.org/10.22456/1679-9216.86667

Abstract

Background: Induction of ovulation is a key procedure for horse assisted reproduction technologies, such as for artificial insemination (AI) and embryo transfer. The application of hCG remains as the primary ovulation-inducing agent for horse assisted reproduction, but alternatives are in demand to avoid its adverse effects, such as loss of ovulation-inducing efficiency over multiple applications by hCC-antibody production. Despite reports on alternative ovulation-inducing agents, pair-wise comparisons of such agents under similar conditions have been limited. Under such scenario, the work was aimed to determine the efficiency of both hCG and Buserelin at inducing ovulation in Mangalarga Marchador mares raised in the Northeast of Brazil under an AI program.

Materials, Methods & Results: Mares were initially selected based on their reproductive performance, the absence of clinical-reproductive alterations and adequate body condition score. Mares in diestrus were randomly distributed in three experimental conditions, received 5 mg of Dinoprost and were monitored daily for estrus detection. After estrus detection, ovaries were monitored by ultrasonography, in 12-h intervals, until the follicle reached 35 mm. At this time-point, ovulation was induced with 0.042 mg of Buserelin endovenously, with 3,000 IU hCG by an intramuscular shot, and control mares received 2 mL of saline solution, also by an intramuscular shot. Both hCG and Buserelin displayed similar efficiencies (P > 0.05) for induction of ovulation and that both agents were effective (P < 0.05) for such purpose, since greater percentages (P < 0.05) of induction on mares treated from those of the control. Moreover, the total number of ovulations in mares treated at the end of the experiment was not different (P > 0.05) from those found in the Control. All ovulations occurred within a 72-h period after treatment. It can be observed that in mares treated with hCG or Buserelin, ovulations occurred both in more mares (P < 0.05) and at earlier time-points than mares from the control. It is also possible to note that pregnancy was not different (P > 0.05) between hCG and Buserelin groups, and that pregnancy of mares treated with ovulation-inducing factors was similar to the control.

Discussion: The majority of ovulations in mares occurred within initial 48-h after treatment for both hCG and GnRH, suggesting a similar potential for horse assisted reproduction. Both hCG and Buserelin are two commonly used agents for induction of ovulation in mares. As described here, the majority of ovulations occurred within initial 48-h after treatment, a fact which can be attributed to hCG and GnRH activity, since it can happen in intervals from 36 to 48-h after treatment. Pregnancy rates did not differ among groups. These results are under the working hypothesis that hCG and Buserelin would display similar efficiencies on pregnancy rates. Despite the understanding of hCG activity on induction of ovulation due to its high specificity toward LH receptors and results from a direct effect on diminishing estradiol concentration, increasing LH, and further inducing ovulation within 48-h after treatment. In contrast, Buserelin has a similar functional property but acts upon LH synthesis and its release. In conclusion, ovulation in mares can be induced with both hCG and Buserelin, and both ovulation-inducing agents do not affect pregnancy rates.

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References

Alvarenga M.A., Carmo M.T. & Landim-Alvarenga F.D.C. 2008. Superovulation in mares: limitations and perspectives. Pferdeheilkunde. 24(1): 88-91.

Awan F.S., Mehmood M.U., Sattar A. & Ahmad N. 2016. Comparative efficacy of hCG or GnRH analogue (lecirelin acetate) on follicular dynamics, degree of endometrial edema, sexual behavior, ovulation and pregnancy rate in crossbred broodmares. Journal of Equine Veterinary Science. 41: 71-72.

Azevedo M.V., Souza N.M., Ferreira-Silva J.C., Batista I.O., Moura M.T., Alvarenga M.A., Oliveira M.A.L. & Lima P.F. 2015. Induction of multiple ovulations in mares using low doses of GnRH agonist Deslorelin Acetate at 48 hours after luteolysis. Pferdeheilkunde. 31(2): 160-164.

Azevedo M.V., Souza N.M., Ferreira-Silva, J.C., Batista I.O., Sales, F.A.B.M., Alvarenga M.A., Oliveira M.A.L. & Lima P.F. 2014. Indução de ovulações duplas utilizando baixa dose de deslorelina em éguas. Ciência Veterinária nos Trópicos. 17(1/2): 42-47.

Barbacini S., Zavaglia G., Gulden P., Marchi V. & Necchi D. 2000. Retrospective study on the efficacy of hCG in an equine artificial insemination programme using frozen semen. Equine veterinary education. 12(6): 312-317.

Barrier-Battut I., Le Poutre N., Trocherie E., Hecht S., des Raux A.G., Nicaise J.L., Vérin X., Bertrand J., Fiéni F., Hoier R., Renault A., Egron L., Tainturier D. & Bruyas J.F. 2001. Use of buserelin to induce ovulation in the cyclic mare. Theriogenology. 55(8): 1679-1695.

Bergfelt D.R. & Adams G.P. 1999. Ovulation synchrony after follicle ablation in mares. Journal of reproduction and fertility. 56(Suppl): 257-269.

Bohme P., Fonseca F.A., da Motta V.A.F. & Espeschit C.J.B. 1992. Eficácia do HMG, HCG e PGF2 alfa na indução ou antecipação da ovulação em éguas durante o cio do potro. Revista Brasileira de Zootecnia. 21(1): 16-22.

Camillo F., Vannozzi I., Tesi M., Sabatini C., Rota A., Paciolla E. & Panzani D. 2014. Induction of ovulation with buserelin in jennies: In search of the minimum effective dose. Animal Reproduction Science. 151(1): 56-60.

Carluccio A., Panzani S., Tosi U., Faustini M., De Amicis I. & Veronesi M.C. 2007. Efficacy of hCG and GnRH for inducing ovulation in the jenny. Theriogenology. 68(6): 914-919.

Cuervo-Arango J. & Newcombe J.R. 2010. Cloprostenol in equine reproductive practice: something more than a luteolytic drug. Reproduction in Domestic Animals. 45(5): 1262-1267.

Duchamp G., Bour B., Combarnous Y. & Palmer E. 1986. Alternative solutions to hCG induction of ovulation in the mare. Journal of Reproduction and Fertility. 35: 221-228.

Farias L.D., Neves A.P., Rechsteiner S.M.D.E.F. & Tarouco A.K. 2016. Indução da ovulação em éguas: uma revisão. Revista Brasileira de Reprodução Animal. 40(1): 17-21.

Fathalla M., Younis L. & Jawad N. 1988. Progesterone concentration and ovascan reading during the estrous cycle in Arabian mares. Journal of Equine Veterinary Science. 8(4): 326-328.

Ginther O.J. 1993. Major and minor follicular waves during the equine estrous cycle. Journal of Equine Veterinary Science. 13(1): 18-25.

Ginther O.J., Beg M.A., Gastal E.L., Gastal M.O. & Cooper D.A. 2009. Treatment with human chorionic gonadotropin (hCG) for ovulation induction is associated with an immediate 17β-estradiol decrease and a more rapid LH increase in mares. Animal Reproduction Science. 114(1): 311-317.

Harrison L.A., Squires E.L. & McKinnon A.O. 1991. Comparison of hCG, buserelin and luprostiol for induction of ovulation in cycling mares. Journal of Equine Veterinary Science. 11(3): 163-166.

Henneke D.R., Potter G.D., Kreider J.L. & Yeates B.F. 1983. Relationship between condition score, physical measurements

and body fat percentage in mares. Equine Veterinary Journal. 15(4): 371-372.

Katila T. 2003. Effects of hormone treatments, season, age and type of mares on ovulation, twinning and pregnancy rates of mares inseminated with fresh and frozen semen. Pferdeheilkunde. 19(6): 619-624.

Leal Fonseca F.C.V., Jacob J.C.F., André M., Sá F., Dutra G.A., Guerson Y.B. & Jesus V.L.T. 2016. Efeito do uso de dinoprost trometamina sobre a taxa de gestação em éguas Mangalarga Marchador. Revista Brasileira de Medicina Veterinária. 38(Suppl 2): 164-168.

McCue P.M., Magee C. & Gee E.K. 2007. Comparison of compounded deslorelin and hCG for induction of ovulation in mares. Journal of Equine Veterinary Science. 27(2): 58-61.

McKinnon A.O., Nobelius A.M., Figueroa S., Skidmore J., Vasey J.R. & Trigg T.E. 1993. Predictable ovulation in mares treated with an implant of the GnRH analogue deslorelin. Equine Veterinary Journal. 25(4): 321-323.

Metcalf E.S. & Thompson M.M. 2010. The effect of PGF2α-induction of estrus on pregnancy rates in mares. Journal of Equine Veterinary Science. 30(4): 196-199.

Monahan M.W., Amoss M.S., Anderson H. & Vale W. 1973. Synthetic analogs of the hypothalamic luteinizing hormone releasing factor with increased agonist or antatonist properties. Biochemistry. 12(23): 4616-4620.

Nagao J.F., Neves Neto J.R., Papa F.O., Alvarenga M.A., Freitas-Dell’Aqua C.P. & Dell’Aqua J.A. 2012. Induction of double ovulation in mares using deslorelin acetate. Animal Reproduction Science. 136(1): 69-73.

Newcombe J.R. & Cuervo-Arango J. 2017. What Are the Options for Induction of Ovulation in the Mare in Europe? Buserelin as an Alternative to Human Chorionic Gonadotropin. Journal of Equine Veterinary Science. 51: 8-17.

Newcombe J.R. & Cuervo-Arango J. 2016. Comparison of the efficacy of different single doses of buserelin with hCG for timed ovulation induction in the mare. Journal of Equine Veterinary Science. 41: 57.

Newcombe J.R. & Cuervo-Arango J. 2011. The effect of time of insemination with fresh cooled transported semen and natural mating relative to ovulation on pregnancy and embryo loss rates in the mare. Reproduction in Domestic Animals. 46(4): 678-681.

Newcombe J.R., Jöchle W. & Cuervo-Arango J. 2008. Effect of dose of cloprostenol on the interval to ovulation in the diestrous mare: a retrospective study. Journal of Equine Veterinary Science. 28(9): 532-539.

Pinto M.R., Miragaya M.H., Burns P., Douglas R. & Neild D.M. 2017. Strategies for Increasing Reproductive Efficiency in a Commercial Embryo Transfer Program With High Performance Donor Mares Under Training. Journal of Equine Veterinary Science. 54: 93-97.

Preacher K.J. 2001. Calculation for the chi-square test: An interactive calculation tool for chi-square tests of goodness

of fit and independence. Computer software. [Fonte: http://quantpsy.org]. [Accessed online in March 2017].

Rodrigues T.G., Caiado J.R.C., Fagundes B. & Straggiotti J.F.S. 2015. Uso de progesterona de longa acao e inovulacao de eguas no segundo dia após a ovulação. Acta Biomedica Brasiliensia. 3(1): 14-26.

Roser J.F., Kiefer B.L., Evans J.W., Neely D.P. & Pacheco C.A. 1979. The development of antibodies to human chorionic gonadotrophin following its repeated injection in the cyclic mare. Journal of Reproduction and Fertility. 27: 173-179.

Samper J.C. 2008. Induction of estrus and ovulation: why some mares respond and others do not. Theriogenology. 70(3): 445-447.

Squires E.L. 2008. Hormonal manipulation of the mare: a review. Journal of Equine Veterinary Science. 28(11): 627-

Squires E.L. & McCue P.M. 2007. Superovulation in mares. Animal Reproduction Science. 99(1): 1-8.

Sullivan J.J., Parker W.G. & Larson L.L. 1973. Duration of estrus and ovulation time in nonlactating mares given human chorionic gondotropin during three successive estrous periods. Journal of the American Veterinary Medical Association. 162(10): 895-898.

Taveiros A.W., Melo P.R.M., Freitas Neto L.M., Aguiar Filho C.R., Silva A.C.J., Lima P.F. & Oliveira M.A.L. 2008. Produção de embriões de éguas Mangalarga Marchador utilizadas nas Regiões Nordeste e Sudeste do Brasil. Medicina Veterinária (UFRPE). 2(3): 19-24.

Voge J.L., Sudderth A.K., Brinsko S.P., Burns P.J. & Blanchard T.L. 2012. Comparison of efficacy of two dose rates of histrelin to human chorionic gonadotropin for inducing ovulation in broodmares. Journal of Equine Veterinary Science. 32(4): 208-210.

Wilson C.G., Downie C.R., Hughes J.P. & Roser J.F. 1990. Effects of repeated hCG injections on reproductive efficiency

in mares. Journal of Equine Veterinary Science. 10(4): 301-308.

Published

2018-01-01

How to Cite

Ferreira-Silva, J. C., Nascimento, P. S., Moura, M. T., Basto, S. R. L., Azevedo, M. V., Rocha, J. M., Santos Filho, J. P., & Oliveira, M. A. L. (2018). Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH. Acta Scientiae Veterinariae, 46(1), 6. https://doi.org/10.22456/1679-9216.86667

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