Electrocardiographic Pattern of Agoutis (Dasyprocta prymnolopha) Chemically Contained by the Association of Ketamine and Xylazine

Authors

  • Osmar Ferreira da Silva Filho Animal Science Post-Graduate Program in Veterinary Image Diagnosis, Federal University of Piauí (UFPI), Teresina, PI, Brazil.
  • Gerson Tavares Pessoa Medical Residence in Veterinary Image Diagnosis, Federal University of Piauí (UFPI), Teresina, PI, Brazil.
  • Renan Paraguassu de Sá Rodrigues Animal Science Post-Graduate Program in Veterinary Image Diagnosis, Federal University of Piauí (UFPI), Teresina, PI, Brazil.
  • Andrezza Braga Soares da Silva Animal Science Post-Graduate Program in Veterinary Image Diagnosis, Federal University of Piauí (UFPI), Teresina, PI, Brazil.
  • Laecio da Silva Moura Animal Science Post-Graduate Program in Veterinary Image Diagnosis, Federal University of Piauí (UFPI), Teresina, PI, Brazil.
  • Francisco das Chagas Araújo Souza Department of Medicine, Faculty of Medical Science, State University of Piauí, (UESPI), Teresina.
  • Maria Angélica Parentes da Silva Barbosa Animal Science Post-Graduate Program in Veterinary Image Diagnosis, Federal University of Piauí (UFPI), Teresina, PI, Brazil.
  • Jacyara de Jesus Rosa Pereira Alves Coloproctology and Colorectal Surgery Service of the University Hospital, UFPI, Teresina.
  • Kassio Vieira Macedo Health Science Post-Graduate, UFPI, Teresina.
  • Flávio Ribeiro Alves Department of Veterinary Morphophysiology, UFPI, Teresina.

DOI:

https://doi.org/10.22456/1679-9216.80208

Keywords:

wild animals, rodents, anesthesia, cardiology.

Abstract

Background: The agouti (Dasyprocta prymnolopha, Wagler, 1831) is an existing wild rodent in almost all of Brazil, used as a biological model in several scientific studies. Veterinary cardiology has showed great advances in the diagnostic area due to the possibility of cardiac evaluation by non-invasive methods. For the practice of scientific or handling procedures in wild animals, chemical containment is essential. Thus, it is important to know the effect of anesthetic protocols on the cardiovascular system, observed through complementary tests, such as the electrocardiogram. The objective of this study was to describe the computerized electrocardiographic tracing of agoutis chemically contained by the association of Ketamine and Xylazine.

Materials, Methods & Results: Eighteen male and female clinically healthy animals, aged among 2 years, submitted to digital electrocardiographic examination, were used. The device used to obtain the tracing was the veterinary electrocardiograph (Electrocardiogram Acquisition Module for Computer) - Brazilian Electronic Technology (TEB). The analysis was always made from derivation II (DII). Chemical containment was performed by the combination of Ketamine and Xylazine, intramuscularly. The results showed that the heart rate for males was on average 113.25 bpm, while for females the value of 124.60 bpm was observed, and there was no significant difference between the genders (P > 0.05). The mean weight of males was 2.31 kg and for females 2.28 kg; there was no statistical difference for this variable (P > 0.05). For the QRS duration of 46.14 ± 5.05 ms (males) and 44.66 ± 5.94 ms (females) and PR interval of 79.94 ± 12.01ms (males) and 84.29 ± 12.37ms (females), there was no statistical difference (P > 0.05). The amplitude of the R wave of 0.42 ± 0.31 mV (males) and 0.36 ± 0.22 mV (females) showed no statistical difference (P > 0.05). The T wave showed itself both negative (10 animals) and positive (8 animals), with amplitude measured at 0.24 ± 0.16 mV for males and 0.25 ± 0.10 mV for females (P > 0.05). The anesthetic protocol was well tolerated by the animals of this experiment, and there were no episodes of arrhythmias during the time of their monitoring.

Discussion: Wild animals, in general, need chemical containment, with a view to reducing stress during scientific and handling procedures. The protocols established in this work can be used in future experiments which require a longer handling time. The values found for heart rate (HR) of anesthetized agoutis were lower than those for non-anesthetized agoutis, proving the influence of the stress during retention and the depressant cardiovascular effect of the anesthetic drug used. The electrocardiographic parameters and the tracing morphology of the agoutis were like the results found for anesthetized small rodents. The QT interval was higher in males than in females, justifying the fact that it could be influenced by variations in HR, which also showed superior results for males. Taking into consideration the morphological similarity between different individuals, the QT interval in anesthetized agoutis did not present significant differences between the genders, a characteristic also found in ferrets. The animals of this experiment were submitted to food fasting, a fact that contributed to a better standardization of the experiment, preventing alterations in the morphology of the QRS complex, which could lead to changes in the measurements.

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References

Allen D.G. & Downey R.S. 1983. Echocardiographic assessment of cats anesthetized with xylazine-sodium pentobarbital. Canadian Journal Compendium. 47(3): 281-283.

Bone L., Battles A.H., Goldfarb R.D., Lombard C.W. & Moreland A.F. 1988. Electrocardiographic values from clinical normal, anesthetized ferrets (Mustela putorious furo). American Journal of Veterinary Research. 49(1): 1884-1887.

Booth N.H. 1992. Anestésicos intravenosos e outros parenterais. In: Booth N.H. & Mcdonald L.E. (Eds). Farmacologia e terapêutica em veterinária. 6.ed. Rio de Janeiro: Guanabara Koogan, pp.168-218.

Bublot I.R., Chalvet-Monfray K. & Edwards N.J. 2006. The surface electrocardiogram in domestic ferrets. Journal of Veterinary Cardiology. 8(2): 87-93.

Carvalho S.F.M. & Santos A.L.Q. 2006. Valores das ondas do eletrocardiograma de Tartarugas-da- Amazônia (Podocnemis expansa, Schweigger, 1812) (testudines). ARS Veterinária. 22(1): 117-121.

Clark D.M., Martin R.A. & Short C.A. 1982. Cardiopulmonary responses to xylazine/ketamine anesthesia in dog. Journal of the American Animal Hospital Association. 18(2): 815-821.

Diniz A.N., Silva-Júnior J.R., Ambrósio C.E., Sousa J.M., Sousa V.R., Carvalho M.A.M., Nascimento D.N. & Alves F.R. 2013. Thoracic and heart biometrics of non-anesthetized agouti (Dasyprocta primnolopha, Wagler, 1831) measured on radiographic images. Pesquisa Veterinária Brasileira. 33(3): 411-416.

Diniz L.S. 1996. Imobilização química em animais silvestres. In: Spinosa H.S., Gorniak S.L. & Bernardi M. (Eds). Farmacologia Aplicada à Medicina Veterinária. 5th edn. Rio de Janeiro: Guanabara Koogan, pp.153-163.

Dudás-Györki Z., Szabó Z., Manczur F. & Vörös K. 2011. Echocardiographic and electrocardiographic examination of clinically healthy, conscious ferrets. Journal of Small Animal Practice. 52(1): 18-25.

Estrada A.H., Gerlach T.J., Schmidt M.K., Siegal-Willott J.L., Adrienne L., Atkins A.L., Scott B., Citino S.B. & Padilla L.R. 2009. Cardiac evaluation of clinically healthy captive maned wolves, Chrysocyon brachyurus. Journal of Zoo and Wildlife Medicine. 40(3): 478-486.

Fantoni D.T. & Cortopassi S.R.G. 2002. Anestesia em Cães e Gatos. 2.ed. São Paulo: Roca, 389p.

Felippe P.A.N. 2007. Eletrocardiografia. In: Cubas Z.S., Silva J.C.R. & Catão-Dias J.L. (Eds). Tratado de animais selvagens medicina veterinária. SãoPaulo: Roca, pp.920-929.

Filippi L.H. 2011. O eletrocardiograma na medicina veterinária. São Paulo: Roca, 160p.

Fortes E.A.M., Ferraz M.S., Bezerra D.O., Conde Júnior A.M., Cabral R.M., Sousa F.C.A., Almeida H.M., Pessoa G.T., Menezes D.J.A., Guerra S.P.L., Sampaio I.B.M., Assis-Neto A.C. & Carvalho M.A.M. 2013. Prenatal development of the agouti (Dasyprocta prymnolopha, Wagler, 1831): External features and growth curves. Animal Reproduction Science. 140(3): 195-205.

Fox M., Brieva C., Moreno C., Macwilliams P. & Thomas C. 2008. Hematologic and Serum Biochemistry Reference Values in Wild-Caught White-Footed Tamarins (Saguinus leucopus) Housed in Captivity. Journal of Zoo and Wildlife Medicine. 39(4): 548-557.

Goodwin J. & Tilley L.T. 2001. Manual of canine and feline cardiology. 5th edn. Philadelphia: WB Saunders, 300p.

Green C.J., Knight J., Precious S. & Simpkin S. 1981. Ketamine alone and combined with diazepam or xylazine in laboratory animals: a 10 year experience. Laboratory Animals. 15(2): 163-17.

Hamlin R.L. & Smith C. R. 1960. Anatomical and physiologic basis for interpretation of the electrocardiogram. American journal of veterinary research. 21(1): 701-708.

Hosken F.M. 2001. Manejo de Cutias. In: Hosken F.M. & Silveira A.C. (Eds). Criação de Cutias. Viçosa: Aprenda Fácil, pp.21-22.

Hunsley M.Z. 2011. Restraint and distress in wild mice: further modification recommended. Laboratory Animal. 40(6): 174-184.

Jacobs G. & Knight D.H. 1985. Change in M-mode echocardiographic values in cats given ketamine. American Journal of Veterinary Reseach. 46(8): 1712-1713.

Kaese S. & Verheule S. 2012. Cardiac electrophysiology in mice: A matter of size. Frontiers in Physiology. 3(1): 345.

Kligfield P., Gettes L.S. & Bailey. 2007. Recommendations for the standardization and interpretation of the electrocardiogram. Part 1: the electrocardiogram and its technology. Circulation. 13(3): 1306-1324.

Kossmann C.E. 1953. The normal electrocardiogram. Circulation. 8(1): 920-936.

Lazzoli J.K., Castro C.L.B., Nóbrega A.C.L. & Araújo C.G.S.A. 2002. Não existe eletrocardiograma de repouso de 12 derivações: uma análise com curvas ROC. Revista Brasileira de Medicina do Esporte. 8(4): 50-58.

Lord B., Boswood A. & Petrie A. 2010. Electrocardiography of the normal domestic pet rabbit. Veterinary Record. 167(25): 961-965.

Martin M. 2002. ECG interpretation in small animals: practical guidelines. In Practice. 24(5): 250-261.

Martin M.W.S. 1995. Small animal echocardiography. In: Goddard P.J. (Ed). Veterinary Ultrasonography. Wallingford: Cab International, pp.131-165.

Massone F. 2003. Anestesiologia Veterinária: Farmacologia e Técnicas. 4.ed. Rio de Janeiro: Guanabara Koogan, 326p.

Migliaro E.R., Contreras P., Bech S., Etxagibel A., Castro M., Ricca R. & Vicente K. 2001. Relative influence of age, resting heart rate and sedentary life style in short-term analysis of heart rate variability. Brazilian Journal of Medical and Biological Research. 34(4): 493-500.

Muir W.W. & Gaynor J.S. 2015. Handbook of veterinary pain management. 2nd edn. St. Louis: Mosby Elsevier, 641p.

Nogueira-Filho S.L.G. & Nogueira S.S.C. 2000. Criação comercial de animais silvestres: produção e comercialização da carne e de subprodutos na região sudeste do Brasil. Revista Econômica do Nordeste. 31(2): 188-195.

Nunes N., Camacho A.A. & Costa J.L.O. 1997. Eletrocardiographic study of the anesthetic combination of ketamine and chlorpromazine HCL in felines. Brazilian Journal of Veterinary Research and Animal Science. 34(1): 313-316.

Oda S.G.S., Yamat R.J., Fedullo J.D.L., Leomil Neto M. & Larsson M.H.M. 2009. Standardization of some electrocardiographic parameters of captive leopard cats, Leopardus tigrinus. Journal of Zoo and Wildlife Medicine. 40(3): 414-420.

Oliveira M.S., Muzzi R.A., Muzzi L.A., Cherem M. & Mantovani M.M. 2014. QT interval in healthy dogs: which method of correcting the QT interval in dogs is appropriate for use in small animal clinics ? Pesquisa Veterinária Brasileira. 34(5): 469-472.

Orutt C.J. 2000. Cardiac and respiratory disease in rabbits. Proceedings of the British Veterinary Zoological Society. 11(1): 68-73.

Pastore C.A., Pinho J.A., Pinho C., Samesima N., Pereira-Filho H.G., Kruse J.C.L., Paixão A., Pérez-Riera A.R., Ribeiro A.L., Oliveira C.A.R., Gomes C.I.G., Kaiser E., Galvão F., Darrieux F.C.C., França F.F.A.C., Feitosa-Filho G, Germiniani H, Aziz J.L., Leal M.G., Molina M, Oliveira N.M.T., Oliveira P.A., Sanches P.C.R., Almeida R.M., Barbosa R., Teixeira R.A., Douglas R.A.G., Gundim R.S. & Atanes S.M. 2016. III Diretrizes da Sociedade Brasileira de Cardiologia sobre Ánalise e Emissão de Laudos Eletrocardiográficos. Arquivos Brasileiros de Cardiologias. 106(4): 3-15.

Prado-Filho O.R., Steffens V.A., Santos B.A., Lukiantchuki L.P.A. & Moreira R.P. 2000. Xilazina como prémedicação para anestesia com tiopental sódico em cães. Acta Cirurgica Brasileira. 15(2): 2-8.

Reusch B. 2005. Investigation and management of cardiovascular disease in rabbits. In Practice. 27(1): 418-25.

Schoemaker N.J. & Zandvliet M.M. 2005. Electrocardiography in Psittacine birds and ferrets. Seminaris in Avian and Exotic Pet Medicine. 14(2): 34-51.

Schumacher J., Snyder P., Scott B., Citino S.B., Bennett R.A. & Dvorak L.D. 2003. Radiographic and electrocardiographic evaluation of cardiac morphology and function in captive cheetahs Acinonyx jubatus. Journal of Zoo and Wildlife Medicine. 34(4): 357-363.

Shukan E. & Hitt N. 2011. Restraint and distress in wild mice: perhaps a pilot study. Labortory Animals. 40(6): 173183.

Slapak L. & Hermanek P. 1957. Observations on the electrocardiogram of rabbits. Zeitschrift für Kreislaufforschung. 46(3-4): 136-142.

J.C., Tranquilli W.J. & Benson C.J. 1999. Essentials of Small AnimalAnesthesia & Analgesia. Philadelphia: Lippincott Williams & Wilkins, 580p.

Tilley L.P. 1992. Essential of canine and feline electrocardiography. 2nd edn. Philadelphia: Lea & Febiger, 470p.

Zandvliet M.J.M. 2005. Electrocardiography in psittacine birds and ferrets. Seminars In Avian and Exotic Pet Medicine. 14(1): 34-51.

Wolf R., Camacho A.A. & Souza R.C.A. 2000. Eletrocardiografia computadorizada em cães. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 52(6): 610-615.

Published

2017-01-01

How to Cite

Filho, O. F. da S., Pessoa, G. T., Rodrigues, R. P. de S., Silva, A. B. S. da, Moura, L. da S., Souza, F. das C. A., Barbosa, M. A. P. da S., Alves, J. de J. R. P., Macedo, K. V., & Alves, F. R. (2017). Electrocardiographic Pattern of Agoutis (Dasyprocta prymnolopha) Chemically Contained by the Association of Ketamine and Xylazine. Acta Scientiae Veterinariae, 45(1), 9. https://doi.org/10.22456/1679-9216.80208

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