A Fatal Pneumonia due to Coinfection of Pseudomonas putida and Staphylococcus pseudintermedius in a Laboratory Beagle Dog


  • Min Hong Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Liping Wei Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Yan Chen Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Yongchang Qin Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Xin Wang Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Yaqun Zhang Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Yan Chang Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.
  • Hua Li Shanghai Innostar Bio-tech Co. Ltd., Shanghai, Shangai, China.




Background: Pseudomonas putida (P. putida) is widely distributed in the environment, and sometimes caused nosocomial infections in human beings, but no case of infection has been reported in beagle dogs. Staphylococcus pseudintermedius (S. pseudintermedius) is a natural cutaneous bacterium in dogs and occasionally causes purulent infections of the skin and rarely causes pneumonia. Both bacteria are opportunistic pathogens. Dogs, even well-controlled laboratory beagle dogs, maybe infected by the bacterium in certain conditions like this report. In order to provide information and give suggestion to veterinarians involved in dogs study, a complete profile of the coinfection was drawn in this report.

Case: It is presented a case of an 8-month-old beagle dog, weighing 6 kg that suffered from coinfection of P. putida and S. pseudintermedius during a treatment of chemotherapy. The animal was confirmed as normal by appearance, physical examination, and laboratory tests before arrival according to the applicable guidelines. After 14-day acclimation period, the animal was administrated with a tyrosinase inhibitor once daily via oral gavage. From Day 8, coughing, decreased activity, hyporeflexia, squinting, shortness of breath (abdominal breathing), and discharge around the nose as well as crackles in the lung and rapid heart rate were noted. Since the poor conditions progressed rapidly and have not been improved by treatment of ceftriaxone and dexamethasone. On Day 9, the animal was euthanized for humanitarian reasons due to rapid progress and poor condition. To define the pathogen, hilar lymph node and thoracic swab were collected for bacteria isolation and purification in special mediums, and at last characterized by Gram staining and 16s rRNA gene sequence analysis and positive PCR-restriction fragment length polymorphism. In clinical pathological examination, an increase in WBC, neutrophils, lymphocytes, monocytes, cholesterol, triglyceride, total protein, globulin, and lactate dehydrogenase, as well as a decrease in RBC, hemoglobin, hematocrit, platelets, sodium ion, chloride ion, and albumin were noted. At necropsy, dark red and enlarged lymph nodes were noted in the hilum of lung, multiple abscesses with yellow pus and multifocal hemorrhage were noted in the lung, and a large amount of frothy yellow fluid were noted in the trachea. In pathological examinations, severe neutrophilic inflammation, diffuse and moderate macrophage aggregation, mild hemorrhage, and moderate alveolar emphysema were noted in the lung, and severe sinusoidal stasis were noted in portal lymph nodes.

Discussion: The current case presented a profile of the appearance, treatment, hematological examination, coagulation examination, clinical chemistry, macroscopic and histological changes in the lung. Multiple purulent abscesses, infiltration of neutrophils, macrophage, and hemorrhage, were correlated to the increase in WBC, neutrophils, lymphocytes, and monocytes, and the decrease in RBC, hemoglobin, hematocrit, and platelets. In the coagulation examination, an increase in Fbg concentration was noted. This change may be induced by the coagulase effect of the S. pseudintermedius, yet no effect on PT or APTT was noted, indicating the coagulation function has not been affected. In the clinical chemistry, the increase of creatine kinase and lactate dehydrogenase may indicate tissue cell damages. Significant increase of globulin may be caused by the inflammatory status. In conclusion, the findings in this case indicate that both Pseudomonas putida and Staphylococcus pseudintermedius can induce infections in laboratory beagle dogs under certain conditions, and might result in a fatal pneumonia which could progress very fast within several days.


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Bardiau M., Yamazaki K., Ote I., Misawa N. & Mainil J.G. 2013. Characterization of methicillin-resistant Staphylococcus pseudintermedius isolated from dogs & cats. Microbiology and Immunology. 57(7): 496-501.

Carlson D., McKeen E., Mitchell M., Torres B., Parad R., Comeau A.M. & O'Sullivan B.P. 2009. Oropharyngeal flora in healthy infants: observations and implications for cystic fibrosis care. Pediatric Pulmonology. 44(5): 497-502.

Carneiro L.E., Vanz A.C. & Barcellos H.H.A. 2018. Tratamento prévio com trilostano e levotiroxina em subdose dificultam diagnóstico de hipotireoidismo canino. Acta Scientiae Veterinariae. 46(1): 304.

Christie I.C., Price J., Edwards L., Muldoon M., Meltzer C.C. & Jennings J.R. 2008. Alcohol consumption and cerebral blood flow among older adults. Alcohol. 42(4): 269-275.

Chrobak D., Kizerwetter-Swida M., Rzewuska M., Moodley A., Guardabassi L. & Binek M. 2011. Molecular characterization of Staphylococcus pseudintermedius strains isolated from clinical samples of animal origin. Folia Microbiologica. 56(5): 415-422.

Chuang C.Y., Yang Y.L., Hsueh P.R. & Lee P.I. 2010. Catheter-related bacteremia caused by Staphylococcus pseudintermedius refractory to antibiotic-lock therapy in a hemophilic child with dog exposure. Journal of Clinical Microbiology. 48(4): 1497-1498.

Fujita J., Negayama K. Ohara M., Hojo S., Obayashi Y., Miyawaki H., Yamaji Y. & Takahara J. 1998. Pneumonia caused by Pseudomonas putida with a mucoid phenotype. Respiratory Medicine. 92(4): 693-695.

George S.E. 2009. Survival of Environmental Microbial Agents in CD-1 Mice Following Oral Exposure. Microbial Ecology in Health and Disease. 12(2): 92-98.

Lombardi G., Luzzaro F., Docquier J.D., Riccio M.L., Perilli M., Coli A., Amicosante G., Rossolini G.M. & Toniolo A. 2002. Nosocomial infections caused by multidrug-resistant isolates of Pseudomonas putida producing VIM-1 metallo-beta-lactamase. Journal of Clinical Microbiology. 40(11): 4051-4055.

Matchett C.A., Dillehay D.L., Goodman M.M. & Pullium J.K. 2003. Postanesthesia death and suspected peracute endotoxic shock due to Pseudomonas putida in a cynomolgous macaque (Macaca fascicularis). Comparative Medicine. 53(3): 309-312.

Rehbinder C., Baneux P., Forbes D., van Herck H., Nicklas W., Rugaya Z. & Winkler G. 1998. FELASA recommendations for the health monitoring of breeding colonies and experimental units of cats, dogs and pigs. Report of the Federation of European Laboratory Animal Science Associations (FELASA) Working Group on Animal Health. Laboratory Animals. 32(1): 1-17.

Stegmann R., Burnens A., Maranta C.A. & Perreten V. 2010. Human infection associated with methicillin-resistant Staphylococcus pseudintermedius ST71. The Journal of Antimicrobial Chemotherapy. 65(9): 2047-2048.

Wan J. 2014. A case of methicillin-resistant Staphylococcus pseudintermedius (MRSP) pyoderma in a Labrador retriever dog. The Canadian Veterinary Journal. 55(11): 1100-1101.

Weese J.S., Faires M., Brisson B.A. & Slavic D. 2009. Infection with methicillin-resistant Staphylococcus pseudintermedius masquerading as cefoxitin susceptible in a dog. Journal of the American Veterinary Medical Association. 235(9): 1064-1066.

Ya-Jiao D., Xiao-Li H., Gui-Ran Z., Wei F., Jing D., Yi-Lin Z., Yong-Qiang D., Yi G., De-Fang C. & Kai-Yu W. 2017. Isolation, identification and pathological lesions of Pseudomonas putida from hybrid catfish. Acta Hydrobiologica Sinica. 41(2): 371-378.

Yamamoto S. & Harayama S. 1998. Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. International Journal Systematic Bacteriology. 48(3): 813-819.

Yang C.H., Young T., Peng M.Y. & Weng M.C. 1996. Clinical spectrum of Pseudomonas putida infection. Journal of the Formosan Medical Association = Taiwan yi zhi. 95(10): 754-761.

Yoshino Y., Kitazawa T., Kamimura M., Tatsuno K., Ota Y. & Yotsuyanagi H. 2011. Pseudomonas putida bacteremia in adult patients: five case reports and a review of the literature. Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy. 17(2): 278-282.

Zakosek Pipan M., Svara T., Zdovc I., Papic B., Avbersek J., Kusar D. & Mrkun J. 2019. Staphylococcus pseudintermedius septicemia in puppies after elective cesarean section: confirmed transmission via dam's milk. BMC Veterinary Research. 15(1): 41.



How to Cite

Hong, M., Wei, L., Chen, Y., Qin, Y., Wang, X., Zhang, Y., Chang, Y., & Li, H. (2021). A Fatal Pneumonia due to Coinfection of Pseudomonas putida and Staphylococcus pseudintermedius in a Laboratory Beagle Dog. Acta Scientiae Veterinariae, 49. https://doi.org/10.22456/1679-9216.107684