B-mode and Doppler Ultrasonographic Assessment of Uterine Involution in Ewes Treated with Two Different Doses of ProstaglandinF2α

Authors

  • Gamze Evkuran Dal Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine & Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey.
  • Sinem Ozlem Enginler Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine & Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey.
  • Ali Can Cetin Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine & Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey.
  • Kerem Baykal Institute of Graduate Education, Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey.
  • Ahmet Sabuncu Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine &Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey.

DOI:

https://doi.org/10.22456/1679-9216.105041

Abstract

Background: Several studies consisted of postmortem evaluations and B-mode ultrasonography have been performed on ovine uterine involution. However, researches with Doppler ultrasonography are very limited in ewes. Doppler ultrasonography is a non-invasive method which provides information about vascular dynamics of the tissues which cannot be examined by B-mode ultrasonography. The aim of the study was to evaluate the effects of two different PGF doses on uterine measurements by real time B-mode ultrasonography; and on uterine artery hemodynamics by pulsed-wave Doppler analysis during postpartum uterine involution in ewes.

Materials, Methods & Results: The study was conducted with 30 primiparous Kiwircik ewes which lambed singleton without any complication. The ewes were randomly divided into three groups (n= 10 for each group). A single i.m. injection of 125 μg/sheep PGF, 75 μg/sheep PGF, and 1.0 mL/sheep sterile saline solution were administered to Group 1, 2, and 3, respectively. The day of parturition was considered as the first day of the study. Examinations were performed on days 1, 2, 3, 7, 14, 21 and 28. Diameters of previously gravid horn and caruncles were measured by real time B-mod ultrasonography. Presence of lochia was also noted. Uterine artery pulsatility index (PI), resistance index (RI), systolic/diastolic ratio (S/D) values were measured by pulsed-wave Doppler ultrasonography. The one-way analysis of variance and Duncan’s test were used for statistical analysis. The mean previously gravid horn diameters of ewes were 8.30 ± 0.16 cm and 1.53 ± 0.07 cm on day 1 and day 28, respectively. Previously gravid horn and caruncle diameters had a similar declining pattern in all groups as involution period proceeded. Involution was mostly completed by day 21. More than 50% reduction in uterine size was achieved in prostaglandin administered groups by day 7. Caruncles were not able to be identified after the second week postpartum. Lochia was observed for a longer period in control group. Uterine artery PI and RI showed fluctuations throughout involution period with a similar pattern among groups. S/D values progressively increased until day 14, then showed a decreasing pattern.

Discussion: The effects of different PGF doses on uterine measurements and uterine artery hemodynamics during postpartum period were compared in ewes for the first time. The uterine size reduction in prostaglandin administered groups suggested that both PGF doses were effective in uterine involution. The last observation of lochia in uterine cavity were achieved by day 7 in prostaglandin administered groups, suggesting both PGF doses trigger uterine contractions which lead to the removal of uterine content. Automatic measurements by Doppler device were taken in order to provide uniformity and to prevent operator-based bias. The significantly higher uterine artery PI value found in Group 1 on day 1 might suggest the stimulatory effect of PGF administration on uterine contractility synergitically with already released endogenous PGF. Uterine artery PI and RI showed fluctuations throughout involution period and reached their peak values on day 14. The increasing S/D values up to day 14 indicated increasing diastolic flow, increasing resistance and decreasing blood perfusion in prostaglandin administered groups. Prostaglandin administered groups tended to show higher hemodynamic parameters throughout the study which suggested a vasoconstrictor effect of PGF. In conclusion, PGF administration on the day of parturition might have constrictor effects both on uterine artery and myometrium which leads to a reduction in uterine blood flow and a rapid decline in uterine size especially during early puerperium even with a reduced dose.

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References

Abecia J.A., Forcada F. & Gonzalez-Bulnes A. 2011. Pharmaceutical control of reproduction in sheep and goats. Veterinary Clinics of North America: Food Animal Practice. 27(1): 67-79. DOI: 10.1016/j.cvfa.2010.10.001

Ahmed N., Ynzeel J.H. & Majeed A.F. 2016. Ultrasonograpic study of uterine involution in of Awassi ewes in Irag. Al-Anbar Journal of Veterinary Sciences. 9(1): 16-21.

Beltrame R.T., Covre C., Littig L.B., Martins A.B., Quirino C.R., Bartholazzi Junior A. & Costa R.L.D. 2017. Transrectal Doppler sonography of uterine blood flow in ewes during pregnancy. Theriogenology. 91: 55-61. DOI: 10.1016/j.theriogenology.2016.12.026

Beltrame R.T., Littig L.B., Covre C., Martins A.B., Quirino C.R. & Costa R.L.D. 2017. Automatic and manual Doppler velocimetry measurements of the uterine artery in pregnant ewes. Animal Reproduction Science. 181: 103-107. DOI: 10.1016/j.anireprosci.2017.03.021

Clark K.E., Austin J.E. & Seeds A.E. 1982. Effect of bisenoic prostaglandins and arachidonic acid on the uterine vasculature of pregnant sheep. American Journal of Obstetrics & Gynecology. 142(3): 261-268. DOI: 10.1016/0002-9378(82)90728-1

Clark K.E., Austin J.E. & Stys S.J. 1981. Effect of bisenoic prostaglandins on the uterine vasculature of nonpregnant sheep. Prostaglandins. 22(3): 333-348. DOI: 10.1016/0090-6980(81)90096-4

Douglas R.H. & Ginther O.J. 1973. Luteolysis following a single injection of prostaglandin F2α in sheep. Journal of Animal Science. 37(4): 990-993. DOI: 10.2527/jas1973.374990x

Edmondson M.A., Roberts J.F., Baird A.N., Bychawski S. & Pugh D.G. 2012. Theriogenology of Sheep and Goats. In: Pugh D.G. & Baird A.N. (Eds). Sheep and Goat Medicine. 2nd edn. St. Louis: Elsevier Saunders, pp.150-230.

Elmetwally M. & Bollwein H. 2017. Uterine blood flow in sheep and goats during the peri-parturient period assessed by transrectal Doppler sonography. Animal Reproduction Science. 176: 32-39. DOI: 10.1016/j.anireprosci.2016.11.005

Elmetwally M., Rohn K. & Meinecke-Tillman S. 2016. Noninvasive color Doppler sonography of uterine blood flow throughout pregnancy in sheep and goats. Theriogenology. 85(6): 1070-1079. DOI: 10.1016/j.theriogenology.2015.11.018

Erdogan G. 2018. Using of Doppler ultrasonography in veterinary gynecology. Turkiye Klinikleri Veterinary Sciences-Obstetrics and Gynecology - Special Topics. 4(1): 43-49.

Erdogan G., Cetin H., Ceylan A., Serin İ. & Beceriklisoy H.B. 2016. Comparison of foetal growth in singleton and twin pregnancies by B-mode and Doppler ultrasonography in Kayra ewes. Turkish Journal of Veterinary and Animal Sciences. 40(5): 616-621. DOI: 10.3906/vet-1508-85

Fernandes C.E., Cigerza C.F., Pinto G.D.S., Miazi C., Barbosa-Ferreira M. & Martins C.F. 2013. Parturition characteristics and uterine involution in native sheep from Brazilian Pantanal. Ciência Animal Brasileira. 14(2): 245-252. DOI: 10.5216/cab.v14i2.17926

Fierro S., Vinoles C. & Olivera-Muzante J. 2016. Concentrations of steroid hormones, estrus, ovarian and reproductive responses in sheep estrous synchronized with different prostaglandin-based protocols. Animal Reproduction Science. 167: 74-82. DOI: 10.1016/j.anireprosci.2016.02.009

Fredriksson G. 1985. Release of PGF2α during parturition and the postpartum period in the ewe. Theriogenology. 24(3): 331-335. DOI: 10.1016/0093-691x(85)90224-9

Ginther O.J. 2007. Producing Spectral Graphs. In: Ultrasonic Imaging and Animal Reproduction: Color-Doppler Ultrasonography Book 4. Cross Plains: Equiservices Publishing, pp.61-86.

Gurbulak K., Pancarci S.M., Gungor O., Kacar C., Oral H., Kirmizigul A.H., Kamiloglu N.N., Karapehlivan M. & Kaya D. 2005. Postpartum involution in winter-lambing Tuj breed sheep and effects of subclinical hypocalsemia on uterine involution in Tuj breed sheep. Kafkas Universitesi Veteriner Fakultesi Dergisi. 11(1): 55-59.

Harrington K., Cooper D., Lees C., Hecher K. & Campbell S. 1996. Doppler ultrasound of the uterine arteries: the importance of bilateral notching in the prediction of pre-eclampsia, placental abruption or delivery of a small-for-gestatinal-age baby. Ultrasound in Obstetrics & Gynecology. 7: 182-188. DOI: 10.1046/j.1469-0705.1996.07030182.x

Hauser B. & Bostedt H. 2002. Ultrasonographic observations of the uterine regression in the ewe under different obstetrical conditions. Journal of Veterinary Medicine Series A. 49(10): 511-516. DOI: 10.1046/j.1439-0442.2002.00496.x

Hayder M. & Ali A. 2008. Factors affecting the postpartum uterine involution and luteal function of sheep in the subtropics. Small Ruminant Research. 79(2-3): 174-178. DOI: 10.1016/j.smallrumres.2008.07.023

Ingoldby L. & Jackson P. 2001. Induction of parturition in sheep. In Practice. 23(4): 228-231. DOI: 10.1136/inpract.23.4.228

Ioannidi K.S., Mavrogianni V.S., Valasi I., Barbagianni M.S., Vasileiou N.G.C., Amiridis G.S., Fthenakis G.C. & Orfanou D.C. 2017. Ultrasonographic examination of the uterus of ewes during the post-partum period. Small Ruminant Research. 152: 74-85. DOI: 10.1016/j.smallrumres.2016.12.014

Ioannidi K.S., Vasileiou N.G.C., Barbagianni M.S., Orfanou D.C., Chouzouris T.M., Dovolou E., Chatzopoulos D.C., Karavanis E., Papadopoulos N., Fthenakis G.C., Amiridis G.S. & Mavrogianni V.S. 2020. Clinical, ultrasonographic, bacteriological, cytological and histological findings during uterine involution in ewes with pregnancy toxaemia and subsequent reproductive efficiency. Animal Reproduction Science. 218: 106460. DOI: 10.1016/j.anireprosci.2020.106460

Light J.E., Silvia W.J. & Reid R.C. 1994. Luteolytic effects of prostaglandin F2α and two metabolites in ewes. Journal of Animal Science. 72(10): 2718-2721. DOI: 10.2527/1994.72102718x

McEntee K. 1990. The Uterus: Normal Postpartum Involution. In: Reproductive Pathology of Domestic Mammals. San Diego: Academic Press, pp.125-141.

Medan M.S. & EL-Daek T. 2015. Uterine involution and progesterone level during the postpatum period in Barbary ewes in North Libya. Open Veterinary Journal. 5(1): 18-22.

Meinecke-Tillman S. 2017. Basics of ultrasonographic examination in sheep. Small Ruminant Research. 152: 10-21. DOI: 10.1016/j.smallrumres.2016.12.023

Noakes D.E. 2019. Physiology of the puerperium. In: Noakes D.E., Parkinson T.J. & England G.C.W. (Eds). Arthur’s Veterinary Reproduction and Obstetrics. 10th edn. Beijing: Elsevier, pp.148-156.

Olivera-Muzante J., Fierro S., Lopez V. & Gil J. 2011. Comparison of prostaglandin- and progesterone-based protocols for timed artificial insemination in sheep. Theriogenology. 75(7): 1232-1238. DOI: 10.1016/j.theriogenology.2010.11.036

Petridis I.G., Barbagianni M.S., Ioannidi K.S., Samaras E., Fthenakis G.C. & Vloumidi E.I. 2017. Doppler ultrasonographic examination in sheep. Small Ruminant Reseach. 152: 22-32. DOI: 10.1016/j.smallrumres.2016.12.015

Pope W.F. & Cardenas H. 2004. Sensitivity of sheep to exogenous prostaglandin F2α early in the estrous cycle. Small Ruminant Research. 55(1-3): 245-248. DOI: 10.1016/j.smallrumres.2004.01.004

Risvanli A., Demiral O., Abay M., Saat N., Bekyurek T., Kulahci F., Niksaroglu S. & Balci T.A. 2010. Effect of different forms of prostaglandin F2α analogues administrationon hormonal profile, prostaglandin F2α binding rate and reproductive traits in Akkaraman sheep during the breeding season. Acta Scientiae Veterinariae. 38(4): 391-398.

Russel A.J., Doney F.J.M. & Gunn R.G. 1969. Subjective assessment of fat in live sheep. The Journal of Agricultural Science. 72(3): 451-454. DOI: 10.1017/S0021859600024874

Scott P.R. 2012. Applications of diagnostic ultrasonography in small ruminant reproductive management. Animal Reproduction Science. 130(3-4): 184-186. DOI: 10.1016/j.anireprosci.2012.01.013

Sheldon I.M., Noakes D.E., Bayliss M. & Dobson H. 2003. The effect of oestradiol on postpartum uterine involution in sheep. Animal Reproduction Science. 78(1-2): 57-70. DOI: 10.1016/S0378-4320(03)00048-4

Turna Yilmaz O., Gündüz M.C., Evkuran Dal G., Ucmak M., Günay Ucmak Z., Karacam E., Kasikci G. & Kilicarslan R.M. 2017. Evaluation of changes in Doppler ultrasonography indices and levels of maternal serum angiogenic factors throughout pregnancy in ewes. Theriogenology. 89: 183-191. DOI: 10.1016/j.theriogenology.2015.09.010

Unal B., Bagcier S., Simsir I., Bilgili Y. & Kara S. 2004. Evaluation of differences between observers and automatic-manual measurements in calculation of Doppler parameters. American Journal of Ultrasound in Medicine. 23(8): 1041-1048. DOI: 10.7863/jum.2004.23.8.1041

Veiga G.A.L., Angrimani D.S.R., Silva L.C.G., Regazzi F.M., Lucio C.F. & Vannucchi C.I. 2018. Hemodynamics of the uterine and umbilical arteries during the perinatal period in ewes. Animal Reproduction Science. 198: 210-219. DOI:10.1016/j.anireprosci.2018.09.021

Wade D.E. & Lewis G.S. 1996. Exogenous prostaglandin F2α stimulates utero-ovarian release of prostaglandin F2α in sheep: a possible component of the luteolytic mechanism of action of exogenous prostaglandin F2α. Domestic Animal Endocrinology. 13(5): 383-398. DOI: 10.1016/0739-7240(96)00069-0

Zdunczyk S., Milewski S., Baranski W., Janowski T., Szczepanski W., Jurczak A., Ras A. & Lesnik M. 2004. Postpartum uterine involution in primiparous and pluriparous Polish Longwool sheep monitored by ultrasonography. Bulletin of the Veterinary Institute in Pulawy. 48: 255-257.

Published

2020-01-01

How to Cite

Evkuran Dal, G., Enginler, S. O., Cetin, A. C., Baykal, K., & Sabuncu, A. (2020). B-mode and Doppler Ultrasonographic Assessment of Uterine Involution in Ewes Treated with Two Different Doses of ProstaglandinF2α. Acta Scientiae Veterinariae, 48. https://doi.org/10.22456/1679-9216.105041

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