Guanidinoacetate alters antioxidant defenses and butyrylcholinesterase activity in the blood of rats

Autores

  • Guilherme André Eger Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil
  • Vinícius Vialle Ferreira Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil
  • Camila Ribeiro Batista Department of Medicine, Regional University of Blumenau, Rua Antônio da Veiga, 140, Blumenau, SC, Brazil
  • Daniela Delwing de Lima Department of Medicine, University of Joinville Region, Campus Universitário, Bairro Bom Retiro, Joinville, SC, Brazil
  • Angela Terezinha de Souza Wyse Department of Biochemistry, Federal University of Rio Grande do Sul, Ramiro Barcelos, 2600-Anexo, Porto Alegre, RS, Brazil
  • Júlia Niehues da Cruz Medical Clinic, Hans Dieter Schmidt Hospital, Rua Xavier Arp s/n°, Joinville, SC, Brazil
  • Débora Delwing Dal Magro Department of Natural Sciences, Regional University of Blumenau, Rua Antônio da Veiga, Blumenau, SC, Brazil
  • José Geraldo Pereira da Cruz Department of Natural Sciences, Regional University of Blumenau, Rua Antônio da Veiga, Blumenau, SC, Brazil

Palavras-chave:

Butyrylcholinesterase, glutathione, guanidinoacetate, L-NAME, TBARS, trolox

Resumo

Deficiency of guanidinoacetate methyltransferase, the first described creatine biosynthesis defect, leads to depletion of creatine and phosphocreatine, and accumulation of guanidinoacetate (GAA) in brain and body fluids. The present study aimed to investigate the influence of GAA on the activities of antioxidant enzymes, as well as on thiobarbituric acid-reactive substances (TBARS) and butyrylcholinesterase (BuChE) activity in the blood of rats. Results showed that GAA enhanced the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in the erythrocytes and BuChE activity. In addition, GAA enhanced TBARS levels in the plasma. Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), GSH (glutathione) and L-NAME (NG-nitro-L-arginine methyl ester) addition prevented the majority of alterations in oxidative stress parameters and the increase of BuChE activity that were caused by GAA. Data suggest that GAA alters antioxidant defenses and induces lipid peroxidation in the blood, as well altering BuChE activity. However, in the presence of trolox, GSH and L-NAME some of these alterations in oxidative stress and BuChE activity were prevented. Our findings lend support to a potential therapeutic strategy for this condition, which may include the use of appropriate antioxidants for ameliorating the damage caused by GAA.

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Publicado

2015-03-11

Como Citar

1.
Eger GA, Ferreira VV, Batista CR, Lima DD de, Wyse AT de S, Cruz JN da, Dal Magro DD, Cruz JGP da. Guanidinoacetate alters antioxidant defenses and butyrylcholinesterase activity in the blood of rats. Clin Biomed Res [Internet]. 11º de março de 2015 [citado 24º de junho de 2022];35(1). Disponível em: https://seer.ufrgs.br/index.php/hcpa/article/view/52197

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