Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested

Authors

  • Tiago Bottin Coser Pontifícia Universidade Católica do Rio Grande do Sul
  • Marisa Chesky Hospital de Clínicas de Porto Alegre
  • Fernanda de-Paris Hospital de Clínicas de Porto Alegre
  • Afonso Luis Barth Hospital de Clínicas de Porto Alegre
  • Virginia Minghelli Schmitt PUCRS
  • Alice Beatriz Mombach Pinheiro Machado Hospital de Clínicas

Keywords:

HBV, PCR, Hepatite B

Abstract

All over the world, the hepatitis B virus (HBV) is considered one of the major problems of public health, despite vaccination. World Health Organization (WHO) estimates that more than 2 billions of persons are infected by HBV. Brazil is classified as an area of intermediary incidence by WHO. However, prevalence studies have detected differences of infection indexes in geographic re-gions: 8% in the Amazonian region, 2,5% in middle-west and Northeast, 2% in Southeast and 1% in South. A sensitive and specific diagnosis is very important to the HBV carrier patients. The aim of this study was to determine the minimum limit of detection of the nested PCR in house technique for HBV. Serial dilutions of one quantified sample of HBV (1000 copies/mL; 750 copies/mL; 500 copies/mL; 250 copies/mL) were submitted to a nested PCR. The target of PCR was viral core and pre-core region. Commercial kit, QiAmp, was employed to purify nucleic acids from the sample. The minimum detection limit found was 500 copies/mL or 10 copies per PCR reaction.

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Author Biographies

Virginia Minghelli Schmitt, PUCRS

Professora Doutora da Faculdade de Farmácia da PUCRS

Alice Beatriz Mombach Pinheiro Machado, Hospital de Clínicas

Atualmente trabalhando no laboratório de Biologia Molecular do Serviço de Patologia Clínica do HCPA (21018860).

Published

2008-06-03

How to Cite

1.
Coser TB, Chesky M, de-Paris F, Barth AL, Schmitt VM, Pinheiro Machado ABM. Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested. Clin Biomed Res [Internet]. 2008Jun.3 [cited 2022May24];28(1). Available from: https://seer.ufrgs.br/index.php/hcpa/article/view/2945

Issue

Section

Original Articles