Comparison Between Qualitative Polymerase Chain Reaction and pp65 Antigenemia for the Diagnosis of Cytomegalovirus Infection in Immunosuppressed Patients

Authors

  • Ricardo Obalski de Mello Centro de Diagóstico Clínico
  • Patrícia Borba Martiny
  • Juliana Belo Saturi
  • Fernanda de Paris Hospital de Clínicas de Porto Alegre
  • Alice Beatriz Pinheiro Machado Hospital de Clínicas de Porto Alegre
  • Martha Bergman Senger Hospital de Clínicas de Porto Alegre
  • Maria Clara Medina Corrêa Hospital de Clínicas de Porto Alegre
  • Luiz Carlos Werres Júnior Hospital de Clínicas de Porto Alegre
  • Graziela Turra Hospital de Clínicas de Porto Alegre
  • Carolina Fischinger Moura de Souza Hospital de Clínicas de Porto Alegre

Keywords:

Citomegalovírus, PCR, antigenemia, reação em cadeia, polimerase

Abstract

Introduction: Cytomegalovirus (CMV) infection is frequent and has significant morbidity and mortality in immunosuppressed individuals, especially in transplanted and HIV-positive patients with advanced disease. Objective: This study compared the performance of qualitative polymerase chain reaction (PCR) and pp65 antigenemia methods for the diagnosis of CMV infection in immunosuppressed patients. Methods: A total of 216 of peripheral blood samples were collected from 85 inpatients or outpatients, from August 2006 through January 2007 from Hospital de Clínicas de Porto Alegre (HCPA). Results: Of the 216 samples, 35 had positive results and 181 were negative with regard to one and/or another technique. Of the 35 positive samples, 16 were positive in both techniques; 12 were positive for PCR and negative for antigenemia, and seven samples were positive for antigenemia but negative for PCR. Considering antigenemia as the gold standard, the PCR technique showed sensitivity of 69.6%, specificity of 93.8%, positive predictive value of 57.1%, and negative predictive value of 96.3%. The kappa correlation coefficient was 0.578. Discussion: These results demonstrate that the qualitative PCR has moderate sensitivity and high specificity for the diagnosis of CMV infection. Despite its limitations, it can be used for diagnosis exclusion in patients under CMV infection suspicion because of its simplicity, low cost and of easy execution.

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Author Biographies

Ricardo Obalski de Mello, Centro de Diagóstico Clínico

Farmacêutico-Bioquímico. Especialista em Análises Clínicas, Universidade Federal do Rio Grande do Sul (UFRGS) em parceria com o Hospital de Clínicas de Porto Alegre (HCPA), RS.

Patrícia Borba Martiny

Farmacêutica-Bioquímica, Aluna de Graduação da Pontifícia Universidade Católica, Porto Alegre, RS.

Juliana Belo Saturi

Farmacêutica-Bioquímica, Aluna de Graduação da Pontifícia Universidade Católica, Porto Alegre, RS.

Fernanda de Paris, Hospital de Clínicas de Porto Alegre

Farmacêutica-Bioquímica contratada, Unidade de Microbiologia e Biologia Molecular, Serviço de Patologia Clínica do HCPA. Mestre em Ciências Biológicas, Área de Bioquímica, UFRGS, Porto Alegre, RS.

Alice Beatriz Pinheiro Machado, Hospital de Clínicas de Porto Alegre

Farmacêutica-Bioquímica contratada, Unidade de Microbiologia e Biologia Molecular, Serviço de Patologia Clínica do HCPA. Mestre em Ciências Médicas, UFRGS, Porto Alegre, RS.

Martha Bergman Senger, Hospital de Clínicas de Porto Alegre

Farmacêutica-Bioquímica contratada, Unidade de Bioquímica e Imunoensaios, Serviço de Patologia Clínica do HCPA. Graduada pela UFRGS, Porto Alegre, RS.

Maria Clara Medina Corrêa, Hospital de Clínicas de Porto Alegre

Farmacêutica-Bioquímica contratada, Unidade de Bioquímica e Imunoensaios, Serviço de Patologia Clínica do HCPA. Graduada pela UFRGS, Porto Alegre, RS.

Luiz Carlos Werres Júnior, Hospital de Clínicas de Porto Alegre

Farmacêutico-Bioquímico contratada, Unidade de Bioquímica e Imunoensaios, Serviço de Patologia Clínica do HCPA. Graduado pela UFRGS, Porto Alegre, RS.

Graziela Turra, Hospital de Clínicas de Porto Alegre

Farmacêutica-Bioquímica, Aluna do Curso de Especialista em Análises Clínicas, Universidade Federal do Rio Grande do Sul em parceria com o Hospital de Clínicas de Porto Alegre, RS.

Carolina Fischinger Moura de Souza, Hospital de Clínicas de Porto Alegre

Médica Geneticista contratada, Serviço de Patologia Clínica do HCPA. Doutorado em Ciências Biológicas, Área de Biologia Molecular, UFRGS, Porto Alegre, RS.

Published

2008-05-06

How to Cite

1.
Mello RO de, Martiny PB, Saturi JB, Paris F de, Machado ABP, Senger MB, Corrêa MCM, Werres Júnior LC, Turra G, Souza CFM de. Comparison Between Qualitative Polymerase Chain Reaction and pp65 Antigenemia for the Diagnosis of Cytomegalovirus Infection in Immunosuppressed Patients. Clin Biomed Res [Internet]. 2008May6 [cited 2022May23];28(1). Available from: https://seer.ufrgs.br/index.php/hcpa/article/view/2598

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Section

Original Articles