DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD DETERMINATION OF ZIDOVUDINE ENCAPSULATED IN PCL NANOPARTICLES

Authors

  • Milena Cristina Ribeiro Souza Magalhães
  • Alisson Samuel Portes Caldeira
  • Hanna de Sousa Rocha Almeida
  • Sílvia Ligório Fialho
  • Armando da Silva Cunha Junior

DOI:

https://doi.org/10.22456/2527-2616.79216

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of encapsulation efficiency of zidovudine in nanoparticules. The method was carried out in isocratic mode using 0.040M sodium acetate: methanol: acetonitrile: glacial acetic acid (880:100:20:2) as mobile phase, a C8 column at 25ºC and UV detection at 240 nm. The method was linear (r2 ˃ 0.99) over the range of 25.0-150.0 μg/mL, precise (RSD ˂ 5%), accurate (recovery = 100.5%), robust and selective. The validated HPLC-UV method can be successfully applied to determine the rate of zidovudine in nanoparticules.

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Published

2017-12-28

How to Cite

Ribeiro Souza Magalhães, M. C., Portes Caldeira, A. S., de Sousa Rocha Almeida, H., Ligório Fialho, S., & da Silva Cunha Junior, A. (2017). DEVELOPMENT AND VALIDATION OF A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD DETERMINATION OF ZIDOVUDINE ENCAPSULATED IN PCL NANOPARTICLES. Drug Analytical Research, 1(2), p. 1–8. https://doi.org/10.22456/2527-2616.79216

Issue

Section

ORIGINAL ARTICLES