Evaluation of guanidine antifungal solutions for denture base resin: an in vitro study

Maria Eduarda Rodrigues Gama, Vicente Castelo Branco Leitune, Isadora Martini Garcia, Stéfani Becker Rodrigues, Fabrício Mezzomo Collares


Background: The purposes of this study were: (1) to formulate polyhexamethylene guanidine hydrochloride (PHMGH) solutions at different concentrations; (2) to evaluate their antifungal activity against a mature biofilm of Candida albicans on acrylic resins; (3) to evaluate possible effects on acrylic resins flexural strength and surface roughness. Methods: PHMGH solutions were formulated with distilled water and 0.125, 0.250, or 0.5 wt.% of PHMGH. One group without PHMGH was used as control. For antifungal activity analysis, acrylic resin specimens were contaminated with C. albicans. Specimens were immersed in PHMGH solutions or distilled water for 5 or 10 min. Ultimate flexural strength and surface roughness of acrylic resins were evaluated. Results: All PHMGH solutions at 5 or 10 min showed antifungal activity compared to the control group (p<0.05). The group with 0.5 wt.% of PHMGH showed no countable colonies regardless of the time of contact with the acrylic resins. After 10 min, all PHMGH solutions had antifungal effect without differences from 0.125 to 0.5 wt.% of PHMGH. All groups showed high flexural strength after contact with the solutions compatible with ISO 20795-1:2013 recommendation. The values of surface roughness always remained low, from 0.01 to 0.04 μm for all groups. Conclusion: Within the limitations of this study, it was concluded that the use of a solution composed by distilled water and 0.5 wt.% of PHMGH for 5 min was effective as a disinfectant agent against mature biofilm of C. albicans , maintaining acceptable roughness and flexural strength.


Acrylic resins; Antifungal agents; Candidiasis; Guanidine; Dentures

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Dagistan S, Aktas AE, Caglayan F, Ayyildiz A, Bilge M. Differential diagnosis of denture-induced stomatitis, Candida, and their variations in patients using complete denture: a clinical and mycological study. Mycoses. 2009;52(3):266-71.

Kamonkhantikul K, Arksornnukit M, Takahashi H. Antifungal, optical, and mechanical properties of polymethylmethacrylate material incorporated with silanized zinc oxide nanoparticles. Int J Nanomedicine. 2017;12:2353-60.

Figueiral MH, Azul A, Pinto E, Fonseca PA, Branco FM, Scully C. Denture-related stomatitis: identification of aetiological and predisposing factors - a large cohort. J Oral Rehabil. 2007;34(6):448-55.

Wilke M, Grube R. Update on management options in the treatment of nosocomial and ventilator assisted pneumonia: review of actual guidelines and economic aspects of therapy. Infect Drug Resist. 2013;7:1-7.

O'Donnell LE, Smith K, Williams C, Nile CJ, Lappin DF, Bradshaw D, et al. Dentures are a reservoir for respiratory pathogens. J Prost. 2016;25(2):99-104.

Schwendicke F, Stolpe M, Muller F. Professional oral health care for preventing nursing home-acquired pneumonia: a cost-effectiveness and value of information analysis. J Clin Periodontol. 2017;44(12):1236-44.

van der Maarel-Wierink CD, Vanobbergen JN, Bronkhorst EM, Schols JM, de Baat C. Oral health care and aspiration pneumonia in frail older people: a systematic literature review. Gerodontology. 2013;30(1):3-9.

Fernandes FH, Orsi IA, Villabona CA. Effects of the peracetic acid and sodium hypochlorite on the colour stability and surface roughness of the denture base acrylic resins polymerised by microwave and water bath methods. Gerodontology. 2013;30(1):18-25.

Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol. 1995;22(1):1-14.

Faot F, Cavalcanti YW, Bertolini MM, Pinto LR, Silva WJ, Del Bel Cury AA. Efficacy of citric acid denture cleanser on the Candida albicans biofilm formed on poly(methyl methacrylate): effects on residual biofilm and recolonization process. BMC Oral Health. 2014;14(77):1-7.

Pawashe KG, Tewary S, Sanyal PK, Nilesh K. An in vitro comparative evaluation of disinfectants on standard and clinical microbial strains on heat cure resins. J Clin Diagn Res. 2017;11(5):ZC54-ZC58.

Amin F, Rehman A, Abbas M. Microhardness of heat cure acrylic resin after treatment with disinfectants. J Pak Med Assoc. 2015;65(8):834-7.

Paranhos Hde F, Peracini A, Pisani MX, Oliveira Vde C, de Souza RF, Silva-Lovato CH. Color stability, surface roughness and flexural strength of an acrylic resin submitted to simulated overnight immersion in denture cleansers. Braz Dent J. 2013;24(2):152-6.

Somkuwar S, Mishra SK, Agrawal B, Choure R. Comparison of the flexural strength of polymethyl methacrylate resin reinforced with multiwalled carbon nanotubes and processed by conventional water bath technique and microwave polymerization. J Indian Prosthodont Soc. 2017;17(4):332-9.

Collares FM, Rostirolla FV, Macêdo EOD, Leitune VCB, Samuel SMW. Influence of mouthwashes on the physical properties of orthodontic acrylic resin. Braz J Oral Sci. 2014;13:203-8.

Goiato MC, Dos Santos DM, Andreotti AM, Nobrega AS, Moreno A, Haddad MF, et al. Effect of beverages and mouthwashes on the hardness of polymers used in intraoral prostheses. J Prosthodont. 2014;23(7):559-64.

Lee HH, Lee CJ, Asaoka K. Correlation in the mechanical properties of acrylic denture base resins. Dent Mater J. 2012;31(1):157-64.

Aviv O, Amir N, Laout N, Ratner S, Basu A, Domb AJ. Poly(hexamethylene guanidine)-poly(ethylene glycol) solid blend for water microbial deactivation. Polym Degrad Stabil. 2016;129:239-45.

Choi H, Kim K-J, Lee DG. Antifungal activity of the cationic antimicrobial polymer-polyhexamethylene guanidine hydrochloride and its mode of action. Fungal Biology. 2017;121(1):53-60.

Oulé MK, Azinwi R, Bernier A-M, Kablan T, Maupertuis A-M, Mauler S, et al. Polyhexamethylene guanidine hydrochloride-based disinfectant: a novel tool to fight meticillin-resistant Staphylococcus aureus and nosocomial infections. J Med Microbiol. 2008;57(12):1523-8.

Oule MK, Quinn K, Dickman M, Bernier AM, Rondeau S, De Moissac D, et al. Akwaton, polyhexamethylene-guanidine hydrochloride-based sporicidal disinfectant: a novel tool to fight bacterial spores and nosocomial infections. J Med Microbiol. 2012;61(Pt 10):1421-7.

Zhou Z, Wei D, Lu Y. Polyhexamethylene guanidine hydrochloride shows bactericidal advantages over chlorhexidine digluconate against ESKAPE bacteria. Biotechnol Appl Biochem. 2015;62(2):268-74.

Garcia IM, Rodrigues SB, Leitune VCB, Collares FM. Antibacterial, chemical and physical properties of sealants with polyhexamethylene guanidine hydrochloride. Braz Oral Res. 2019;33:1-9.

Collares FM, Garcia IM, Bohns FR, Motta AdSd, Melo MA, Leitune VCB. Guanidine hydrochloride polymer additive to undertake ultraconservative resin infiltrant against Streptococcus mutans. Eur Polym J. 2020;133:109746.

Garcia IM, Rodrigues SB, Rodrigues Gama ME, Branco Leitune VC, Melo MA, Collares FM. Guanidine derivative inhibits C. albicans biofilm growth on denture liner without promote loss of materials' resistance. Bioact Mater. 2020;5(2):228-32.

Choure RB, Sthapak A, Yadav NS, Srivastava T, Ali SS, Dixit S. Effect of alcohol and tea on solubility of soft-liner and polymethyl methacrylate resin: an in vitro study. J Contemp Dent Pract. 2019 Jan;20(1):83-8.

Lima JFM, Maciel JG, Hotta J, Vizoto ACP, Honório HM, Urban VM, et al. Porosity of temporary denture soft liners containing antifungal agents. J Appl Oral Sci. 2016;24(5):453-61

Kreve S, Dos Reis AC. Denture liners: a systematic review relative to adhesion and mechanical properties. ScientificWorldJournal. 2019;2019:6913080.

Moura JS, da Silva WJ, Pereira T, Del Bel Cury AA, Rodrigues Garcia RC. Influence of acrylic resin polymerization methods and saliva on the adherence of four Candida species. J Prosthet Dent. 2006;96(3):205-11.

International Organization for Standardization (ISO). Dentistry-base polymers:20795-1:2013.2013:1-35.

Park K. An analysis of a humidifier disinfectant case from a toxicological perspective. Environ Health Toxicol. 2016;31:e2016013.

Koo H, Andes DR, Krysan DJ. Candida–streptococcal interactions in biofilm-associated oral diseases. PLoS Pathog. 2018;14(2):e1007342.

Souza JGS, Bertolini M, Thompson A, Mansfield JM, Grassmann AA, Maas K, et al. Role of glucosyltransferase R in biofilm interactions between Streptococcus oralis and Candida albicans. ISME J. 2020;14(5):1207-22.

Bowen WH, Burne RA, Wu H, Koo H. Oral Biofilms: Pathogens, Matrix, and Polymicrobial Interactions in Microenvironments. Trends Microbiol. 2018;26(3):229-42.

Sharma P, Garg S, Kalra NM. Effect of denture cleansers on surface roughness and flexural strength of heat cure denture base resin-an in vitro study. J Clin Diagn Res. 2017;11(8):ZC94-7.

Glauser S, Astasov-Frauenhoffer M, Muller JA, Fischer J, Waltimo T, Rohr N. Bacterial colonization of resin composite cements: influence of material composition and surface roughness. Eur J Oral Sci. 2017;125(4):294-302.

DOI: https://doi.org/10.22456/2177-0018.104096

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