Development of resin-based bioactive endodontic cements with glycerol salicylate and calcium silicate

Rafaela Cassaro Pistorello, Gabriela de Souza Balbinot, Vicente Castelo Branco Leitune, Fabricio Mezzomo Collares


Introduction: The combination of sol-gel derived calcium silicate particles and glycerol salicylate resins may enhance the pyhisico-chemical properties contribute to the understanding of the interaction between these materials. This study aims to evaluate the physical-chemical properties of resin-based bioactive endodontic cements with glycerol salicylate resins and calcium silicate particles. Materials and methods: Calcium silicate was produced by the sol-gel route, while the resin was produced by mixing 60wt% glycerol salicylate, 30wt% methyl salicylate and 10wt% distilled water. Calcium silicate was added in three different concentrations, 50, 40 and 25% by weight. The cement was tested for setting time, flow, radiopacity and pH. Results: The 50:50 group shows the time of 15min8s while the 75:25 shows the time of 256min13s (p<0.05). The 50:50 group has a lower flow (15.156mm) compared to 60:40 (23.588 mm) and 75:25 (25.396 mm). All radiopacity values were below 3mmAl. All groups showed a pH increase up to 24 hours and the pH value drop was inversely proportional to the amount of calcium silicate. Discussion: Bioactive calcium silicate particles were used in a composite material with a glycerol salicylate resin. Among the tested cements, the combination of 50wt% calcium silicate particles to 50wt% glycerol salicylate resin showed adequate setting time and promoted an increase in water pH. Conclusion: The 50:50 group showed the setting time and the pH, showing that these materials may be able to promote enhanced biological response. The adjustment of flow and radiopacity should be considered for its clinical application.


Regenerative endodontics; Dental cements; Resin cements; Glycerol; Biocompatible materials

Full Text:



Torabinejad M, Parirokh M, Dummer PMH. Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview - part II: other clinical applications and complications. Int Endod J. 2018 Mar;51(3):284–317.

Bakhtiar H, Nekoofar MH, Aminishakib P, Abedi F, Naghi Moosavi F, Esnaashari E, et al. Human pulp responses to partial pulpotomy treatment with theracal as compared with biodentine and proroot mta: a clinical trial. J Endod. 2017 Nov;43(11):1786–91.

Bonte E, Beslot A, Boukpessi T, Lasfargues J-J. MTA versus Ca(OH)2 in apexification of non-vital immature permanent teeth: a randomized clinical trial comparison. Clin Oral Investig. 2015 Jul;19(6):1381–8.

Mahmoud SH, El-Negoly SA, Zaen El-Din AM, El-Zekrid MH, Grawish LM, Grawish HM, et al. Biodentine versus mineral trioxide aggregate as a direct pulp capping material for human mature permanentteeth: asystematic review. J Conserv Dent. 2018 Oct;21(5):466–73.

Camilleri J, Sorrentino F, Damidot D. Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater. 2013 May 1;29(5):580–93.

Athanasiadou E, Paschalidou M, Theocharidou A, Kontoudakis N, Arapostathis K, Bakopoulou A. Biological interactions of a calcium silicate based cement (Biodentine™) with stem cells from human exfoliated deciduous teeth. Dent Mater. 2018 Dec 1;34(12):1797–813.

Balbinot G de S, Leitune VCB, Nunes JS, Visioli F, Collares FM. Synthesis of sol–gel derived calcium silicate particles and development of a bioactive endodontic cement. DentMater [Internet]. 2019 Nov 26 [cited 2019 Nov 27]; Available from:

Kim YK, Grandini S, Ames JM, Gu L, Kim SK, Pashley DH, et al. Critical review on methacrylate resin-based root canal sealers. J Endod. 2010 Mar;36(3):383–99.

Gong S-Q, Huang Z-B, Shi W, Ma B, Tay FR, Zhou B. In vitro evaluation of antibacterial effect of AH Plus incorporated with quaternary ammonium epoxy silicate against Enterococcus faecalis. J Endod. 2014 Oct;40(10):1611–5.

Akcay M, Arslan H, Topcuoglu HS, Tuncay O. Effect of calcium hydroxide and double and triple antibiotic pastes on the bond strength of epoxy resin-based sealer to root canal dentin. J Endod. 2014 Oct;40(10):1663–7.

Portella FF, Santos PD, Lima GB, Leitune VCB, Petzhold CL, Collares FM, et al. Synthesis and characterization of a glycerol salicylate resin for bioactive root canal sealers. IntEndod J. 2014 Apr 1;47(4):339–45.

Portella FF, Collares FM, Dos Santos LA, dos Santos BP, Camassola M, Leitune VCB, et al. Glycerol salicylate-based containing α-tricalcium phosphate as a bioactive root canal sealer. J Biomed Mater Res Part B Appl Biomater. 2015 Nov;103(8):1663–9.

Yu P, Xia C-J, Li D-D, Wang Z, Xiao W, Zhao L-G. Structural optimization of caffeoyl salicylate scaffold as no production inhibitors. Chem Pharm Bull. 2019;67(9):1006–14.

Zeng C, Wei J, Persson MSM, Sarmanova A, Doherty M, Xie D, et al. Relative efficacy and safety of topical non-steroidal anti-inflammatory drugs for osteoarthritis: a systematic review and network meta-analysis of randomised controlled trials and observational studies. Br J Sports Med. 2018 May;52(10):642–50.

ISO 6876:2012(en), Dentistry — Root canal sealing materials [Internet]. [cited 2016 Jun 19]. Available from:

Camilleri J, Sorrentino F, Damidot D. Characterization of un-hydrated and hydrated BioAggregateTM and MTA AngelusTM. Clin Oral Investig. 2015 Apr;19(3):689–98.

Darvell BW, Wu RCT. “MTA”-an Hydraulic Silicate Cement: review update and setting reaction. Dent Mater. 2011 May 1;27(5):407–22.

Setbon HM, Devaux J, Iserentant A, Leloup G, Leprince JG. Influence of composition on setting kinetics of new injectable and/or fast setting tricalcium silicate cements. Dent Mater. 2014 Dec 1;30(12):1291–303.

Ridi F, Fratini E, Luciani P, Winnefeld F, Baglioni P. Hydration kinetics of tricalcium silicate by calorimetric methods. J Colloid Interface Sci. 2011 Dec 1;364(1):118–24.

Siew K, Lee AHC, Cheung GSP. Treatment outcomeofrepaired root perforation: a systematic review and meta -analysis. J Endod. 2015 Nov;41(11):1795–804.

Emara R, Elhennawy K, Schwendicke F. Effects of calcium silicate cements on dental pulpcells: a systematic review. J Dent. 2018 Oct 1;77:18–36.

Sultana N, Singh M, Nawal RR, Chaudhry S, Yadav S, Mohanty S, et al. Evaluation of biocompatibility and osteogenic potential of tricalcium silicate-based cements using human bone marrow-derived mesenchymal stem cells. J Endod. 2018 Mar;44(3):446–51.

Trongkij P, Sutimuntanakul S, Lapthanasupkul P, Chaimanakarn C, Wong RH, Banomyong D. Pulpal responses after direct pulp capping with two calcium-silicate cements in a rat model. Dent Mater J. 2019 May;38(4):584-90.

Camilleri J, Gandolfi MG. Evaluation of the radiopacity of calcium silicate cements containing different radiopacifiers. IntEndod J. 2010;43(1):21–30.

Costa BC, Guerreiro-Tanomaru JM, Bosso-Martelo R, Rodrigues EM, Bonetti-Filho I, Tanomaru-Filho M, et al. Ytterbium Oxide as radiopacifier of calcium silicate-based cements. Physicochemical and biological properties. Braz Dent J. 2018 Sep;29(5):452–8.

Marciano MA, Costa RM, Camilleri J, Mondelli RFL, Guimarães BM, Duarte MAH. Assessment of color stability of white mineral trioxide aggregate angelus and bismuth oxide in contact with tooth structure. J Endod. 2014 Aug;40(8):1235–40.


Licença Creative Commons

e-ISSN 2177-0018 / ISSN 0566-1854.

descrição da foto descrição da foto descrição da foto descrição da foto descrição da foto descrição da foto descrição da fotodescrição da foto