Intervals of Semen Collection from Boars during Early Reproductive Life: Evaluation of Seminal Fluid Parameters

Julianni Dornelles, Carlos Augusto Rigon Rossi, Luara Medianeira de Lima Schlösser, Rodrigo Dalmina Rech, Cristian Guilherme Gräf, Cínthia Melazzo de Andrade, Marcelo Soares, Marcos Speroni Ceron, Jovani Patias

Abstract


Background: In swine production, good reproduction rates can be achieved through genetic selection and reproductive biotechnologies. One of these biotechnologies is artificial insemination, which contributes to disseminate genes and optimize breeding boars, thus improving the quality of insemination doses. This study focused on evaluating the intervals between semen collection from boars at the beginning of their reproductive maturity vis-à-vis the viability of insemination doses.

Materials, Methods & Results: Twenty 9-month-old boars of the genetic lineage AGPIC 337 (Agroceres PIC) were used in this study. The experimental design used here was completely randomized, and the randomly selected males were divided into four treatment groups, which were named according to the interval between semen sample collections: T1: 2 days; T2: 3 days; T3: 4 days and T4: 7 days. Each treatment comprised 5 animals, and at the end of the 90 days of this study, a total of 150 ejaculates were obtained in T1, 110 in T2, 90 in T3 and 60 in T4. The values of total motility, volume and sperm concentration of the ejaculates were evaluated, as was oxidative stress by means of thiobarbituric acid reactive substances (TBARS), the latter after 0, 72, 120 and 168 h of the study. Membrane integrity was evaluated at 0, 72, 120 and 168 h using the eosin-nigrosin staining procedure. Sperm heat resistance was tested after 120 h, and sperm morphology after 72, 120 and 168 h. Sperm concentrations differed, with T3 showing 27.04% and 29.65% higher concentrations (P < 0.05) than groups T2 and T1, respectively. Total motility in group T4 was 0.56%, 1.98% and 3.28% higher (P < 0.05) than in T3, T2 and T1, respectively, indicating that the 7-day interval produced the best result. The heat resistance test showed the expected results, i.e., T2 and T4 did not differ in terms of total motility, but that of T4 was 4.96% higher (P < 0.05) than T3 and 7.71% higher than T1. As for plasma membrane integrity, T4 had 6.45%, 8.09% and 12.72% more cells with intact membranes (P < 0.05) than T3, T2 and T1, respectively. With regard to TBARS, group T1 showed higher concentrations (P < 0.05) than T2 (6.18%), T3 (11.13%) and T4 (9.12%), i.e., the sperm cells in the animals of T1 showed greater changes. As for sperm morphology, the number of intact cells in T4 was 6.08%, 7.53% and 12.44% higher (P < 0.05) than in T3, T2 and T1, respectively.

Discussion: With respect to total motility, other authors have reported similar results, i.e., the motility of sperm was highest when collected at 7-day intervals and lower when collected at shorter intervals. Despite the statistical differences between treatments, these results were found to be similar to those recommended in the literature for use in the preparation of insemination doses. Thus, we suggest that all the intervals between collections can be used in the routine of semen production centers, since the minimum requirement for an ejaculate to be accepted in an artificial insemination program is to have a total motility of no less than 70 percent. At the beginning of their reproductive maturity, boars may be subjected to intervals between collections of at least 2 days, without impairing the total motility of insemination doses. However, the best results are obtained when semen is collected at 7-day intervals, which ensures higher total motility, morphologically healthy cells with intact membranes, and less oxidative damage caused by lipid peroxidation.


Full Text:

PDF

References


Andrade E.R., Melo-Sterza F.A., Seneda M.M. & Alfieri A.A. 2010. Consequências da produção das espécies reativas de oxigênio na reprodução e principais mecanismos antioxidantes. Revista Brasileira de Reprodução Animal. 34(2): 79-86.

Badell S.S. 2002. Efectes del fotoperíode sobre la qualitat espermàtica de mascles porcins sus domesticus. Girona, Espanha. Tese (Doutorado em Biologia) - Programa de Pós-Graduação em Biologia, Universidade de Girona.

Bernardi M.L. 2008. Tecnologias aplicadas no exame do ejaculado suíno para a produção de doses de sêmen de alta qualidade. Acta Scientiae Veterinariae. 36(Supl 1): 5-16.

Bonet S., Casas I., Holt W.V. & Yeste M. 2013. Boar Reproduction, Fundamentals and New Biotechnological Trends. New York: Springer, 634p.

Bortolozzo F.P., Wentz I., Ferreira F.M., Bennermann P.E. & Bernardi M.L. 2005. Exame do ejaculado. In.: Bortolozzo F.P., Wentz I., Bennemann P.E., Bernardi M.L., Wollmann E.B., Ferreira F.M. & Borchardt Neto G. (Eds). Inseminação Artificial na Suinocultura Tecnificada. Porto Alegre: Pallotti, pp.69-90.

Colégio Brasileiro de Reprodução Animal (CBRA). 2013. Manual para exame andrológico e avaliação de sêmen animal. 3.ed. Belo Horizonte: CBRA, 104p.

Cruz D.F., Lume C., Silva J.V., Nunes A., Castro I., Silva R. & Silva V. 2015. Oxidative stress markers: can they be used to evaluate human sperm quality? Turkish Journal of Urology. 41(4): 198-207.

Ferreira F.M., Wentz I., Scheid I.R., Afonso S.B., Guidoni A.L. & Bortolozzo F.P. 2005. Comportamento de monta e características seminais de suínos jovens landrace e large white. Ciência Rural. 35(1): 131-137.

Flowers W.L. 1997. Management of boars for efficient semen production. Journal of Reproduction and Fertility. (Suppl 52): 67-78.

Frangez R., Gider T. & Kosec M. 2005. Frequency of boar ejaculate collection and its influence on semen quality, pregnancy rate and litter size. Acta Veterinariae Brno. 74: 265-273.

Knecht D., Jankowska-Mąkosa A. & Duziński K. 2017. The effect of age, interval collection and season on selected semen parameters and prediction of AI boars productivity. Livestock Science. 201(9): 13-21.

Marques I.S., Paiva B.G., Durães M.J.O., Souza D.C., Barbosa G.F. & Marques Filho W.C. 2018. Efeito da frequência de coleta sobre a qualidade do sêmen suíno. In: VII Seminário de Iniciação Científica do IFNMG (Araçuaí, Brasil). pp.1-3.

Mellagi A.P.G., Quirino M., Oliveira G.S., Gaggini T.S., Paschoal A.F.L., Lucca M.S., Ulguim R.R. & Bortolozzo F.P. 2019. Atualizações na avaliação andrológica em suínos. Revista Brasileira de Reprodução Animal. 43(2): 47-53.

Pruneda A., Pinart E., Briz M.D., Sancho S., Garcia-Gil N., Badia E., Kádár E., Bassols J., Bussalleu E., Yeste M. & Bonet S. 2005. Effects of a high semen collection frequency on the quality of sperm from ejaculates and from six epididymal regions in boars. Theriogenology. 63: 2219-2232.

Reis G.R., Bernardi M.L., Schwarz P., Bortolozzo F.P. & Wentz I. 2002. Diferença entre machos suínos na manutenção da viabilidade espermática a 17ºC. Acta Scientiae Veterinariae. 30(3): 159-166.

Rigo V.H.B. 2016. Influência do Resveratrol na Qualidade e na Fertilidade do Espermatozoide Suíno Refrigerado entre 15-17℃ por 72 horas para Inseminação Artificial Intrauterina. 125f. Pirassununga, SP. Dissertação (Mestrado) - Programa de Pós-Graduação em Zootecnia, Universidade de São Paulo.

Rigo V.H.B., Martin S.M.M.K., Nichi M., Freitas F.V. & Andrade A.F.C. 2017. O uso do Resveratrol na conservação do sêmen suíno sob refrigeração. Revista Brasileira de Reprodução Animal. 40(2): 615-619.

Schlling E. & Vengust M. 1987. Frequency of semen collection in boars and quality of ejaculates as evaluated by the osmotic resistance of acrosomal membranes. Animal Reproduction Science. 12: 283-290.

Tanno P.H. 2009. Estudo das alterações morfo-funcionais de espermatozoides bovinos submetidos à sexagem por meio da técnica de citometria de fluxo. 100f. São Paulo, SP. Dissertação (Mestrado em Medicina Veterinária) - Programa de Pós-Graduação em Reprodução Animal, Universidade de São Paulo.




DOI: https://doi.org/10.22456/1679-9216.98315

Copyright (c) 2019 Julianni Dornelles, Carlos Augusto Rigon Rossi, Luara Medianeira de Lima Schlösser, Rodrigo Dalmina Rech, Cristian Guilherme Gräf, Cínthia Melazzo de Andrade, Marcelo Soares, Marcos Speroni Ceron, Jovani Patias

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.