Staphylococcus aureus and Mesophilic Aerobic Bacteria Quantification in Hygienization Process of Milking Equipment

Daniela de Avila Silva Bohrz, Bruna Webber, Franciele Rampazzo Vancin, Luciane Daroit, Fernando Pilotto, Luciana Ruschel dos Santos, Laura Beatriz Rodrigues

Abstract


Background:Milk’s composition is an excellent substrate for microorganism’s multiplication. Presence of Staphylococcus aureus and aerobic mesophilic bacteria are one of the most common problems in dairy farms. On dairy industry’s and milk farms Clean in Place (CIP) system higyenization are commonly used, then the cleaning occurs as a closed process, for better results sanitizans are applied, in order to obtain a safety food. This project aim to evaluate Staphylococcus aureus and aerobic mesophilic bacteria reduction after two milking higyenization process. 

Materials, Methods & Results:This research was done on a Rio Grande do Sul North Milk farm, with mechanized milking and Clean in Place system for cleaning. For liners and CIP tubes higyenization commercial products as Sodium Hipoclorite 3% and phosphoric acid 11.3% are used for detergency, and peracetic acid 5% for sanitization. Milk bunk tank are higyenized with sodium hypoclorite 3.8% alcalin detergent. After higyenization steps liners, CIP’s water process, bulk milk tank and milk set were collected. At process 1, liners and CIP water were collected after milking, detergency and sanitization that occurred immediately at the detergency’s finish, while process 2 the sanitization was realized 8 h after detergency, before following milking. Cooling milk bulk tank was collected before and after detergency, and milk set after milkings Convencional microbiology were used to count and results in log10 UFC.cm-2. In CIP water’s after process 1 was 3.81 log10 reduction to aerobic mesophilic bacteria (P > 0.05) and reducing 4.51 log10 (P = 0.03). Meanwhile there was no significant reduction for mesophilic aerobic bacteria and S. aureus on the others samples (liners, bulk milk tank and milk set).

Discussion: This results show the maintenance of milking machine contamination, and that even bacterial load reduced among hygienization steps this was not significant, suggesting that deteriorate and pathogenic microorganisms can remain on milk produced. Highlights are teat taps of milking machine as the major cause of contamination among cows. The results are worrying because Staphylococcus aureus contamination, once this bacteria causes alimentar diseases, even after higyenization process, which can damage public health that can reflect milk chain economically. Since amount of this microorganism found in milk is already sufficient to synthesize enterotoxins. In addition, resistance to disinfectants is another concern, as it may result in resistance to antimicrobial agents. So reduction of bacteria level among cleaning steps there was no significance, once the products and equipments on dairy and farms act as a constant elimination point of deteriorate and pathogenic microorganisms for the final product, milk. With this studies aim to aprimorate hygienization process on milk chain, in order to obtain a hygienic sanitary good product.



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References


Alvarez N., Daufin G. & Gésan-Guiziou G. 2010. Recommendations for rationalizing cleaning-in-place in the dairy industry: Case study of an ultra-high temperature heat exchanger. Journal of Dairy Science. 93(2): 808-821. doi:10.3168/jds.2009-2760

Andrade N.J. 2008. Higienização na indústria de alimentos: avaliação e controle da adesão e formação de biofilmes bacterianos. São Paulo: Varela. 412p.

Bergdoll M.S. 1990. Staphylococcal food poisoning. In: Cliver D.O. (Ed). Foodborne diseases. London: Academic, pp.87-106.

Brasil. 2011. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa n° 62. Diário Oficial da República Federativa do Brasil. Seção 1, Brasília, 29 de dezembro de 2011.

Brito M.A.V.P., Arcuri E.F. & Brito J.R.F. 2000. Testando a qualidade do leite. In: Durães M.C., Martins C.E., Deresz F., Brito J.R.F., Freitas A.F., Portugal J.A.B. & Costa C.N. (Eds). Avanços tecnológicos para o aumento da produtividade leiteira. Anais Minas Leite. Juiz de Fora: Embrapa Gado de Leite, pp.83-94.

Cavalcanti E.R.C. 2005. Construção do conhecimento sobre o potencial de contaminação em ordenhadeira mecânicas após higienização. 67f. Rio de Janeiro, RJ. Dissertação (Mestrado em Educação Agrícola) - Universidade Federal Rural do Rio de Janeiro.

Costa F.F. 2006. Interferência de práticas de manejo na qualidade microbiológica do leite produzido em propriedades rurais familiares. 80f. Jaboticabal, SP. Dissertação (Mestrado em Zootecnia) - Universidade Estadual Paulista Júlio de Mesquita Filho.

Evancho G.M., Sveum W.H., Moberg L.J. & Frank J.F. 2001. Microbiological Monitoring of the Food-Processing Environment. In: Downes F.P. & Ito K. (Eds). Compendium of Methods for the Microbial Examination of Foods. Washington DC: American Public Health Association, pp.25-35.

Garcia M.E.T.A., Couto E.P & Ferreira M.A. 2014. Leite orgânico produzido no Distrito Federal: avaliação da qualidade físico-química e microbiológica. ASA - Atas de saúde ambiental. 2(3): 16-24.

Gleeson D., O’Brien B., Flynn J., O’Callaghan E. & Galli F. 2009. Effect of pre-milking teat preparation procedures on the microbial count on teats prior to cluster application. Irish Veterinary Journal. 62(7): 461. doi:10.1186/2046-0481-62-7-461

Hoffmann F.L., Coelho A.R., Mansor A.P. & Vinturim T.M. 2002. Avaliação da atividade antimicrobiana in vitro de dois agentes sanificantes de uso industrial. Higiene Alimentar. 16(94): 62-67.

Langsrud S., Sidhu M.S., Heir E. & Holck A.L. 2003. Bacterial disinfectant resistance - a challenge for the food industry. International Biodeterioration & Biodegradation. 51(4): 283-290. doi:10.1016/s0964-8305(03)00039-8

Laranja da Fonseca L.F. & Santos M.V. 2000. Qualidade do leite e controle de mastite. Cap. 14. São Paulo: Ed. Lemos, pp.151-161.

Miguel P.R.R., Pozza M.S.S., Caron L.F., Zambom M.A. & Pozza P.C. 2012. Incidência de contaminação no processo de obtenção do leite e suscetibilidade a agentes antimicrobianos. Semina: Ciências Agrárias. 33(1): 403-416. doi:10.5433/1679-0359.2012v33n1p403

Moretro T., Vestby L.K., Nesse L.L., Storheim S.E., Kotlarz K. & Langsrud S. 2009. Evaluation of efficacy of disinfectants against Salmonella from the feed industry. Journal of Applied Microbiology. 106(3): 1005-1012. doi:10.1111/j.1365-2672.2008.04067.x

Pengov A. 2006. Staphylococcus aureus - do we really have to live with it? Slov Vet Res. 43(1): 41-46.

Picoli S.U., Bessa M.C., Castagna S.M.F., Gottardi C.P.T., Schmidt V. & Cardoso M. 2006. Quantificação de coliformes, Staphylococcus aureus e mesófilos presentes em diferentes etapas da produção de queijo frescal de leite de cabra em laticínios. Ciência e Tecnologia de Alimentos. 26(1): 64-69. doi:10.1590/s0101-20612006000100011

Picoli T., Zani J.L., Bandeira F.S., Büttow V.F., Ribeiro M.E.R., Vargas G.A., Hübner S.O., Lima M., Meireles M.C.A. & Fischer G. 2014. Manejo de ordenha como fator de risco na ocorrência de micro- organismos em leite cru. Semina: Ciências Agrárias. 35(4): 2471-2480. doi:10.5433/1679-0359.2014v35n4Suplp2471.

Reche N.L.M., Thaler Neto A., D`Ovideo L., Felipus N.C., Pereira L.C., Cardozo L.L. & Picinin L.C.A. 2015. Multiplicação microbiana no leite cru armazenado em tanques de expansão direta. Ciência Rural. 45(5): 828-834. doi:10.1590/0103-8478cr20140542

Sabelot M.A., dos Santos Pozza M.S., da Silva L.S., Zambom M.A., Pozza P.C. & Eckstein I.I. 2014. Isolamento de bactérias causadoras de mastite subclínica e correlação entre qualidade físico-química do leite e contagem de células somáticas. Revista de Ciência Veterinária e Saúde Pública. 1(2): 99-106.

Tronco V.M. 2010. Manual para Inspeção de Qualidade do Leite. Santa Maria: Ed. UFSM, 203 p.

Zecchoni A. & Hahn G. 2000. Staphylococcus aureus in raw milk and human health risk. Bulletin IDF. 345: 15-18.




DOI: https://doi.org/10.22456/1679-9216.97777

Copyright (c) 2019 Daniela de Avila Silva Bohrz, Bruna Webber, Franciele Rampazzo Vancin, Luciane Daroit, Fernando Pilotto, Luciana Ruschel dos Santos, Laura Beatriz Rodrigues

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