Risk Factors for Enzootic Leukosis in Dairy Cattle from the Brazilian Amazon

Authors

  • Tamyres Izarelly Barbosa da Silva Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Leonardo Augusto Kohara Melchior Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Priscila Souza Santos Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Guilherme Henrique Reckziegel Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Yuri Almeida Gomes Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Artur Cesar Carvalho Fernandes Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Daniel Dias da Silva Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Luiz Carlos Fontes Baptista Filho Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.
  • Lúcio Esmeraldo Honório de Melo Unidade de Ensino e Pesquisa em Medicina Veterinária, Universidade Federal do Acre (UFAC), Rio Branco, AC, Brazil.

DOI:

https://doi.org/10.22456/1679-9216.96577

Abstract

Background: Enzootic bovine leukosis (EBL) is a viral and lymphoproliferative disease that attributes negative economic impacts to dairy farming. The immunosuppressive condition of these bovines favors the development of bacterial diseases such as tuberculosis, brucellosis, and bovine leptospirosis, which implications to the public health given that they are zoonosis. Diagnosis and control of EBL are neglected in cattle rearing, allowing their dissemination, especially in developing countries. In this sense, the objective of this study was to analyze the risk factors of EBL in dairy cattle from the Brazilian Amazon.
Materials, Methods & Results: In order to identify the BLV-infected animals (527 serological samples), we used the agarose gel immunodiffusion serological test (IDGA) to diagnose the EBL and characterize the risk factors through the application of epidemiological questionnaires. We used the logistic regression test to evaluate the existence of a significant association between the variables. The EBL frequency in the study area was 14.42%. We detected seroreagent bovines in all municipalities evaluated with 95% of the properties showing outbreaks of the disease. The reuse of needles was the risk factor with the most significant statistical association (P < 0.01; OR = 2.16).
Discussion: The origin of EBL in the Brazilian Amazon is probably related to the expansion of dairy cattle in the 1980s, which, at first, demanded the importation of cattle for the fast formation of the herds, without complying with the sanitary criteria of animal commercialization. The frequency of EBL in the Rio Branco microregion is below the estimated national average, as well as the regional level. The reduced influx of animals due to the geographical situation of Acre, since it is an area of greater physical isolation due to forest and river barriers, may influence the observed frequency. Different environmental conditions, herd size, cattle management, genetics and particularities of the diagnostic techniques should also be considered. The Rio Branco microregion can currently be considered as an area of medium frequency for BLV infection, a situation that ranges from 11 to 30% in the occurrence rate. When compared to herds with low infection frequency (<10%), the elimination of seroreagent animals and their respective outbreaks become more expensive in areas of medium or high frequency. As for the number of outbreaks detected, the finding reflects the degree of negligence regarding the prophylactic measures of EBL since it is a silent propagation disease, as well as the implications for the absence of official control and eradication programs in Brazil. According to the final logistic regression model, the reuse of needles for vaccinations, deworming, and for applying other drugs in different animals was the risk factor of greater association in the region, explaining the widespread occurrence of EBL in the studied farms. In Canadian herds, this practice also was associated with an increase in clinical cases. It is known that only 0.1 mL of blood is necessary as a minimal infectious dose of BLV. However, despite the relevance of this variable, it is little studied in epidemiological surveys, and this practice continues to be repeated in cattle. In conclusion, the wide dissemination of EBL in the dairy herds of the region is related to the practice of reusing needles and its relevance in the iatrogenic transmission of the disease

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References

Abreu V.L.V., Modena C.M., Silva J.A., Moreira E.C. & Figueiredo M.M.N. 1990. Prevalência da leucose enzoótica bovina nos estados de Rondônia e Acre. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 42: 203-210.

Acre. Governo do Estado do Acre. 2016. Portal de Informações: Sobre o Acre. Available from: <http://www.ac.gov.br/wps/portal/acre/Acre/home>. [Accessed online in December 2018].

Akagami M., Oya S., Kashima Y., Seki S., Ouchi Y. & Hayama Y. 2019. A hematologic key for bovine leukemia virus screening in Japanese black cattle. Journal of Veterinary Diagnostic Investigation. 31(4):568-571. doi: 10.1177/1040638719848449.

Benitez-Rojas O.J., Roberts J.N., Norby B., Bartlett P.C., Maeroff J.E. & Grooms D.L. 2019. Lack of Bovine leukemia virus transmission during natural breeding of cattle. Theriogenology. 126: 187-190.

Birgel Junior E.H., Dias W.M., Souza R.M., Pogliani F.C., Birgel D.B. & Birgel E.H. 2006. Prevalência da infecção pelo vírus da leucose dos bovinos em animais da raça Simental, criados no estado de São Paulo. Ars Veterinaria. 22: 122-129.

Carneiro P.A.M., Araujo W.P., Birgel E.H. & Souza K.W. 2003. Prevalência da infecção pelo vírus da leucose enzoótica dos bovinos e rebanhos leiteiros criados no Estado do Amazonas. Acta Amazonica. 33: 111-125.

Dohoo I.R., Martin S.W. & Stryhn H. 2009. Veterinary Epidemiologic Research. 2nd edn. Charlottetown: VER Inc., 865p.

Farias M.V.N., Souza F.N., Lendez P.A., Martínez-Cuesta L., Santos K.R., Della Libera A.M.M.P., Ceriani M.C. & Dolcini G.L. 2018. Lymphocyte proliferation and apoptosis of lymphocyte subpopulations in bovine leukemia virus-infected dairy cows with high and low proviral load. Veterinary Immunology and Immunopathology. 206: 41-48.

Fernandes C.H.C., Melo L.E.H., Tenório T.G.S., Mendes E.I., Fernandes A.C.C., Ramalho T.R.R., Sobrinho P.A.M. & Mota R.A. 2009. Soroprevalência e fatores de risco da infecção pelo vírus da leucose dos bovinos em rebanhos leiteiros da região norte do estado do Tocantins, Brasil. Arquivos do Instituto Biológico. 76: 327-334.

Frie M.C. & Coussens P.M. 2015. Bovine leukemia virus: A major silent threat to proper immune responses in cattle. Veterinary Immunology and Immunopathology. 163(3): 103-114.

Frie M.C., Sporer K.R., Wallace J.C., Maes R.K., Sordillo L.M., Bartlett P.C. & Coussens P.M. 2016. Reduced humoral immunity and atypical cell-mediated immunity in response to vaccination in cows naturally infected with bovine leukemia virus. Veterinary Immunology and Immunopathology. 182: 125-135.

Instituto Brasileiro de Geografia e Estatística (IBGE). 2017. Unidades da Federação. Available from: <http://www.ibge.gov.br/estadosat/perfil.php?> [Accessed online in January 2019].

Juliano R.S., Fioravanti M.C.S., Brito W.M.E.D., Abreu U.G.P. & Souza S.N. 2014. Soroepidemiologia da leucemia bovina em bovinos curraleiros dos estados de Goiás e Tocantins, Brasil. Ciência Animal Brasileira. 15(3): 289-295.

Khudhair Y.I., Hasso S.A., Yaseen N.Y. & Al-Shammari A.M. 2016. Serological and molecular detection of bovine leukemia virus in cattle in Iraq. Emerging Microbes & Infections. 5(6): 1-6.

Kobayashi T., Inagaki Y., Ohnuki N., Sato R., Murakami S. & Imakawa K. 2019. Increasing Bovine leukemia virus (BLV) proviral load is a risk factor for progression of Enzootic bovine leucosis: A prospective study in Japan. Preventive Veterinary Medicine. doi: 10.1016/j.prevetmed.2019.04.009. [Epub ahead of print].

Kohara J., Takeuchi M., Hirano Y., Sakurai Y. & Takahashi T. 2018. Vector control efficacy of fly nets on preventing bovine leukemia virus transmission. Journal of Veterinary Medical Science. 80(10): 1524-1527.

Konishi M., Ishizaki H., Kameyama K., Murakami K. & Yamamoto T. 2018. The effectiveness of colostral antibodies for preventing bovine leukemia virus (BLV) infection in vitro. BMC Veterinary Research. 14(419): 1-9.

Kuczewski A., Hogeveen H., Orsel K., Wolf R., Thompson J., Spackman E. & Van Der Meer F. 2019. Economic evaluation of 4 bovine leukemia virus control strategies for Alberta dairy farms. Journal of Dairy Science. 102(3): 1-15.

Lojkić I., Balić D., Rudan N., Kovačić M., Čač Z., Periškić M., Bedeković T., Roić B. & Grozdanić I.C. 2013. Eradication of bovine leukosis virus on a dairy farm through improved virus detection. Veterinarski Arhiv. 83(6): 581-591.

Mendes E.I., Melo L.E.H., Tenório T.G.S., Sá L.M., Souto R.J.C., Fernandes A.C.C., Sandes H.M.M. & Silva T.I.B. 2011. Intercorrência entre leucose enzoótica e tuberculose em bovinos leiteiros do estado de Pernambuco. Arquivos do Instituto Biológico. 78(1): 1-8.

Miller J.M. & Van Der Maaten M.J. 1975. Serological detection of bovine leukemia virus infection. In: Proceedings of the 2nd CEC Seminar on Bovine Leukosis (Copenhagen, Denmark). 1 CD ROM.

Modena C.M., Abreu V.L.V., Silva J.A., Moreira E.C., Azevedo N.A. & Rehfeld O.A.M. 1983. Ocorrência de infecção pelo vírus da leucose enzoótica bovina em animais importados. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 35(4): 565-573.

Molnár E., Molnár L., Dias H.T., Silva O.M. & Vale W.G. 1999. Ocorrência da leucose enzoótica dos bovinos no estado do Pará, Brasil. Pesquisa Veterinária Brasileira. 19: 7-11.

Nekouei O., Vanleeuwen J., Sanchez J., Kelton D., Tiwari A. & Keef G. 2015. Herd-level risk factors for infection with bovine leukemia virus in Canadian dairy herds. Preventive Veterinary Medicine. 119: 105-113.

Nishimori A., Konnai S., Okagawa T., Maekawa N., Goto S., Ikebuchi R., Nakahara A., Chiba Y., Ikeda M., Murata S. & Ohashi K. 2017. Identification of an atypical enzootic bovine leukosis in japan by using a novel classification of bovine leukemia based on immunophenotypic analysis. Clinical and Vaccine Immunology. 24(9): 1-14.

Ochirkhuu N., Konnai S., Odbileg R., Nishimori A., Okagawa T., Murata S. & Ohashi K. 2016. Detection of bovine leukemia virus and identification of its genotype in Mongolian cattle. Archives of Virology. 161(4): 985-991.

Ohno A., Takeshima S.N., Matsumoto Y. & Ainda Y. 2015. Risk factors associated with increased bovine leukemia virus proviralload in infected cattle in Japan from 2012 to 2014. Virus Research. 210: 283-290.

Organização Mundial de Saúde Animal (OIE). 2012. Código terrestre de saúde animal. Leucose Enzoótica Bovina. Available from: <http://www.oie.int> [Accessed online in January 2019].

Ott S.L., Johnson R. & Wells S.J. 2003. Association between bovine leukosis virus seroprevalence and herd-level productivity on US dairy farms. Preventive Veterinary Medicine. 61(4): 249-262.

Panei C.J., Larsen A.E., Fuentealba N.A., Metz G.E., Echeverría M.G., Galosi C.M. & Valera A.R. 2019. Study of horn flies as vectors of bovine leukemia virus. Open Veterinary Journal. 9(1): 33-37.

Pereira A.L.M., Costa A.F., Veschi J.L.A. & Almeida K.S. 2013. Soroprevalência da leucose enzoótica bovina - revisão de literatura. Revista Científica Eletrônica de Medicina Veterinária. 11(21): 1-20.

Polat M., Takeshima S. & Aida Y. 2017. Epidemiology and genetic diversity of bovine leukemia vírus. Virology Journal. 14(209): 1-16.

Rajão D.S. 2008. Efeito da infecção pelo vírus da leucose enzoótica bovina na produção de leite e reprodução de rebanhos leiteiros. 26f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Minas Gerais, Escola de Veterinária, Belo Horizonte.

Ruiz V., Porta N.G., Lomónaco M., Trono K. & Alvarez I. 2018. Bovine leukemia virus infection in neonatal calves. Risk factors and control measures. Frontiers in Veterinary Science. 5: 1-7.

Santos G.R., Oliveira J.M.B., Brandespim D.F., Oliveira A.A.F., Mota R.A. & Pinheiro Júnior J.W. 2013. Análise epidemiológica da infecção pelo vírus da leucose enzoótica bovina (LEB), na microrregião Garanhuns, Pernambuco, Brasil. Revista Brasileira de Medicina Veterinária. 35(4): 371-377.

Santos H.P., Pereira H.M., Nascimento S.A., Coutinho L.C.A., Teixeira W.C., Arruda R.C.N., Bezerra N.P.C., Bezerra D.C. & Castro R.S. 2011. Frequência de anticorpos e fatores de risco associados à leucose enzoótica bovina em rebanhos da bacia leiteira do estado do Maranhão. Arquivos do Instituto Biológico. 78(3): 351-358.

Şevik M., Avcı O. & İnce O.B. 2015. An 8-year longitudinal sero-epidemiological study of bovine leukaemia virus (BLV) infection in dairy cattle in Turkey and analysis of risk factors associated with BLV seropositivity. Tropical Animal Health and Production. 47: 715-720.

Shettigara P.T., Samagh B.S. & Lobinowich E.M. 1986. Eradication of bovine leukemia virus infection in commercial dairy herds using the agar gel immunodiffusion test. Canadian Journal of Veterinary Research. 50: 221-226.

Sun W.W., Lv W.F., Cong W., Meng Q.F., Wang C.F., Shan X.F. & Qian A.D. 2015. Mycobacterium avium subspecies paratuberculosis and bovine leukemia virus seroprevalence and associated risk factors in commercial dairy and beef cattle in northern and northeastern China. BioMed Research International. 1: 1-7.

Tsutsui T., Kobayashi S., Hayama Y. & Yamamoto T. 2016. Fraction of bovine leukemia virus-infected dairy cattle developing enzootic bovine leukosis. Preventive Veterinary Medicine. 124: 96-101.

Published

2019-01-01

How to Cite

Barbosa da Silva, T. I., Kohara Melchior, L. A., Santos, P. S., Reckziegel, G. H., Gomes, Y. A., Carvalho Fernandes, A. C., da Silva, D. D., Baptista Filho, L. C. F., & Honório de Melo, L. E. (2019). Risk Factors for Enzootic Leukosis in Dairy Cattle from the Brazilian Amazon. Acta Scientiae Veterinariae, 47(1). https://doi.org/10.22456/1679-9216.96577

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