Effects of Polyunsaturated Fatty Acids (PUFA) Supplementation on Reproductive Performance of Beef Heifers Submitted to Fixed-time Artificial Insemination (FTAI) Protocol

Authors

  • João Batista Souza Gorges Unidade de Reprodução de Bovinos, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
  • Andressa Varella Gonsioroski Unidade de Reprodução de Bovinos, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
  • Eduardo Pradebon da Silva Unidade de Reprodução de Bovinos, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.

DOI:

https://doi.org/10.22456/1679-9216.95139

Abstract

Background: More recently, polyunsaturated fatty acids (PUFA) supplements have been used in grazing beef herds in attempt to influence specific metabolic pathways and hormones that directly modulate reproductive function. Supplementation of calcium salts of PUFA can positively influence ovarian follicular growth, luteal function, and improve reproductive performance in dairy and beef females. The aim of this experiment was to evaluate strategies of dietary treatments using PUFA supplementation for 30 days before or after FTAI protocol and its effects on of ovulatory follicle size, ovulation, pregnancy and gestational losses rates in beef heifers.

Materials, Methods & Results: One hundred and ninety-nine Brangus heifers (24 ± 3 months-old; BW= 320 ± 15 kg; BCS= 2.85 ± 0.17) were randomly assigned in three different groups to receive dietary treatments. Group 1 (n = 70) was fed with 0.5 kg/day of a protein-mineral mixture supplement containing 0.2 kg/day PUFA (Mix A)during 30 days before FTAI, Group 2 (n = 68) received the same supplement 30 days after FTAI and Control (n = 61) only the supplement without PUFA (Mix B) during all period. Heifers were submitted to estrous synchronization treatment using progesterone (P4), estradiol benzoate (E2) and prostaglandin F2a  (PGF). Ultrasound exams were done to measure ovulatory follicle diameter and to determinate ovulation and pregnancy rates on Day 30 and 60 after FTAI. Daily gain (DG) was higher (P < 0.05) only when heifers received supplement containing PUFA (Group 1= 30 days prior FTAI) and (Group 2= 30 days after FTAI). PUFA supplementation before FTAI also resulted in larger ovulatory follicles diameter (P < 0.05) in Group 1 (11.2 ± 2.5 mm) compared to Group 2 (9.9 ± 2.3 mm) and Control (10.0 ± 2.07 mm). Ovulation rates were similar (P > 0.05) in Group 1 (90%), Group 2 (75%) and Control (67%). Pregnancy rates were higher (P < 0.05) in Group 1 (Day 30= 67.1% and Day 60= 64.2%) when compared with Group 2 (Day 30= 48.5% and Day 60= 44.1%) and Control (Day 30= 49.2% and Day 60= 45.9%). Gestation losses were similar (P > 0.05) among Groups 1 (6.6%), 2 (9%) and Control (6.6%).

Discussion: Rumen-protected supplemental lipids have been used to increase energy density and to determine direct positive effects on reproduction in dairy and beef herds.  There is evidence that feeding moderate amounts of PUFA supplements can improve energy status and result in significant differences in DG as it was observed during experimental period. Thus, heifers that received 0.2 kg/day of PUFA supplement 30 days before FTAI protocol had also a positive effect on ovulatory follicles size, but ovulation rates were similar in groups. Beneficial effects of pre-breeding PUFA supplementation were observed on pregnancy rates on Days 30 and 60 after FTAI, but pregnancy loss was not different among dietary treatments in beef heifers. Based on this study, PUFA supplementation before FTAI protocol had a positive effect on reproductive performance of beef heifers by directly improving ovarian function, uterine environment and early embryonic development. Results indicate that pre-breeding PUFA supplementation to beef heifers is a suitable strategy to increase pregnancy rate in FTAI programs. Therefore, for a better understanding of interactions of nutritional strategies and utero-ovarian functions more evaluations are needed to understand the mechanisms by which feeding PUFA may improve reproductive function in beef heifers.

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References

Ambrose D.J., Kastelic J.P., Corbett R., Pitney P.A., Petit H.V., Small J.A. & Zalkovic P. 2006. Lower pregnancy losses in lactating dairy cows fed a diet enriched in α- linolenic acid. Journal of Dairy Science. 89: 3066-3074.

Bilby T.R., Block J., Amaral B.C., Sá Filho O., Silvestre F.T., Hansen, P.J., Staples C.R. & Thatcher W.W. 2006. Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in summer. Journal of Dairy Science. 89: 3891-3903.

Childs S., Carter F. & Lynch C.O. 2008. Embryo yield and quality following dietary supplementation of beef heifers with n-3 polyunsaturated fatty acids (PUFA). Theriogenology. 70: 992-1003.

Childs S. Lynch C.O. & Hennessy A.A. 2008. Effect of dietary enrichment with either n-3 or n-6 fatty acids on systemic metabolite and hormone concentration and ovarian function in heifers. Animal. 2: 883-893.

Fuston R.N. 2004. Fat supplementation and reproduction in beef females. Journal of Animal Science. 82: 154-161.

Guardiero M.M., Bastos M.R., Mourão G.B., Carrijo L.H.D., Melo E.O., Rumpf R. & Sartori R. 2010. Função ovariana de novilhas Nelore alimentadas com dieta suplementada com gordura protegida ruminal. Pesquisa Agropecuária Brasileira. 45: 408-414.

Kim M. & Kinoshita J.Y. 2001. Lipid and fatty acid analysis of fresh and frozen-thawed immature and in vitro matured bovine oocytes. Reproduction. 122: 131-138.

Lopes C.N., Vasconcelos J.L.M., Araújo T.P.B. & Oliveira L.O.F. 2007. Effect of supplementation with Megalac E on pregnancy rate in primiparous Nellore cows. Journal of Animal Science. 85(suppl. 2): Abstract.

Lopes C.N., Scarpa A.B., Cappellozza B.I., Cooke R.F. & Vasconcelos J.L.M. 2009. Effects of rumen-protected polyunsaturated fatty acid supplementation on reproductive performance of Bos indicus beef cows. Journal of Animal Science. 87: 3935-3943.

Lopes C.N., Cooke R.F., Reis M.M., Peres R.F.G. & Vasconcelos J.L.M. 2011. Strategic supplementation of calcium salts of polyunsaturated fatty acids to enhance reproductive performance of Bos indicus beef cows. Journal of Animal Science. 89: 3116-3124.

Lucy M.C., Staples C.R., Michel F.M. & Thatcher W.W. 1991. Energy balance and size and number of ovarian follicles detected by ultrasonography in early postpartum dairy cows. Journal of Dairy Science. 74: 473-482.

Mann G.E. & Lamming G.E. 1999. The Influence of Progesterone During Early Pregnancy in Cattle. Reproduction of Domestic Animals. 34: 269-274.

Mattos R., Staples C.R. & Thatcher W.W. 2000. Effects of dietary fatty acids on reproduction in ruminants. Reviews of Reproduction. 5: 38-45.

Raes K., De Smet S. & Demeyer D. 2004. Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review. Animal Feed Science and Technology. 13: 199-221.

Robinson R.S., Pushpakumara P.G.A., Cheng Z., Peters A.R., Abayasekara D.R.E. & Wathes D.C. 2002. Effects of dietary polyunsaturated fatty acids on ovarian and uterine function in lactating dairy cows. Reproduction. 124: 119-131.

Ryan D.P., Spoon R.A. & Williams G.L. 1992. Ovarian follicular characteristics, embryo recovery, and embryo viability in heifers fed high-fat diets and treated with follicle-stimulation hormone. Journal of Animal Science. 70: 3505-3513.

Santos J.E.P., Bilby T.R., Thatcher W.W., Staples C.R. & Silvestre F.T. 2008. Long chain fatty acids of diet as factors influencing reproduction in cattle. Reproduction of Domestic Animals. 43 (Supp. 2): 23-30.

Staples C.R., Wiltbank,M.C. & Grummer R.R. 2000. Effect of long chain fatty acids on lactation performance and reproductive tissues of Holstein cows. Journal of Dairy Science. 83: 278.

Staples C.R., Burke J.M. & Thatcher W.W. 1998. Influence of supplemental fats on reproductive tissues and performance of lactating cows. Journal of Dairy Science. 81: 856-871.

Thatcher W.W., Meyer M.D. & Danet-Desnoyers G. 1995. Maternal recognition of pregnancy. Journal of Reproduction & Fertility. 49: 15-28.

Watches D.C., Robert D., Abayasekara E. &Aitken A. 2007. Polyunsaturated fatty acid in male and female reproduction. Biology of Reproduction. 77: 190-201.

Published

2019-01-01

How to Cite

Souza Gorges, J. B., Gonsioroski, A. V., & da Silva, E. P. (2019). Effects of Polyunsaturated Fatty Acids (PUFA) Supplementation on Reproductive Performance of Beef Heifers Submitted to Fixed-time Artificial Insemination (FTAI) Protocol. Acta Scientiae Veterinariae, 47(1). https://doi.org/10.22456/1679-9216.95139

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