Hemogram, Fibrinogen Concentration and Plasma Total Protein from Calves of Criollo Lageano Breed Variety Hornless and Aberdeen Angus Breed (Red Angus) on the First Six Months of Life

Jackson Schade, Renato May Rossi, Mere Erika Saito, Graziela Vieira Fonteque, Thiago Rinaldi Muller, Joandes Henrique Fonteque

Abstract


Background: Neonatal period in bovine presents important variations in hematologic and immunologic parameters, and the resulting mortality rates may be due to increased susceptibility to disease. Race, age, environmental, climate and management factors are sources of CBC’s parameters variation in cattle. The objective of this study is to evaluate and compare the CBC variables and concentrations of total protein (PTP) and plasma fibrinogen calves of Criolo Lageano Breed variety hornless and Aberdeen Angus (Red Angus) in the first six months of life, which are produced under extensive management at the Planalto Serrano Catarinense (southern Brazil).

Materials, Methods & Results: Two groups were selected, comprising 11 calves of Hornless Criolo Lageano and 14 calves of the Aberdeen Angus breed (Red Angus). Blood samples were collected for complete blood count and determination of total protein concentrations, and plasma fibrinogen in the periods between 24 h and 36, 15, 30, 60, 90, 120, 150 and 180 days of life. The statistical analysis was performed using analysis of variance for repeated measures (ANOVA) and Tukey’s test (P < 0.05). During the experimental period infestations by ticks in both races were observed. In the first month of life, blood smears revealed the presence of blood parasites of the genus Babesia spp. and Anaplasma sp. The fluctuations seen for erythrocyte in the neonatal period (30 days), although not significant, consisted of raising the number of values of erythrocytes, hemoglobin (Hb) and packed cell volume (PCV), and reduction in mean corpuscular volume values (MCV). From 90 days of age there was a significant reduction in number of red blood cells and VG values, and elevated MCV values and mean corpuscular volume (MCHC) for the Aberdeen Angus (Red Angus). For the WBC, the neonatal period was marked by increase in the number of lymphocytes and decrease in neutrophil ratio: lymphocyte (N, L) with respect to the inversion of 15 days. In the age group 90 days of life was evident increase in the number of leukocytes and lymphocytes in both races. The plasma fibrinogen concentrations showed no significant variations during the trial period. The plasma total protein (PTP), had higher values on the second day of life, with reductions up to 60 days and gradual increase in subsequent times.

Discussion: The fluctuations related to the values of erythrocyte variables in the neonatal period are consistent with results reported by other authors in calves of other races. Changes in erythrocyte values observed from 90 days of age on race Aberdeen Angus (Red Angus) can be attributed to infection by blood parasites and consequent hemolysis. As for the leucocyte count, increasing the number of lymphocytes and inversion of N:L is the neonatal period in a physiological variation and confirms observations made by other authors in calves of other races. In the age group 90 days, leukocytosis by lymphocytosis was related to natural premonition against Babesia spp. and Anaplasma spp., which is documented in cattle in tropical and subtropical regions. When analyzing the PTP values on the second day of life, it can be said that there has been proper transfer of passive immunity in both races, given the high correlation of PTP values and gamma globulins. The reduction of PTP and subsequent elevation values are characteristic of the physiological curve related to degradation of passively transferred immunoglobulin and immunoglobulin synthesis by competent immune system.


Keywords


bovine; erythrogram; leukogram; fibrinogen; total protein.

Full Text:

PDF

References


Adams R., Garry F.B., Aldridge B.M., Holland M.D. & Odde K.G. 1992. Hematologic values in newborn beef calves. American Journal of Veterinary Research. 53(6): 944-950.

Assis-Brasil N.D., Pereira C.M., Hinnah F.L., Ladeira S.R.L., Sallis E.S.V., Grecco F.B. & Schild A.L. 2013. Enfermidades diagnosticadas em bezerros na região sul do Rio Grande do Sul. Pesquisa Veterinária Brasileira. 33(4): 423-430.

Benesi F.J., Teixeira C.M.C., Lisboa J.A.N., Leal M.L.R., Birgel Júnior E.H., Bohland E. & Mirandola R.M.S. 2012. Eritrograma de bezerras sadias da raça Holandesa no primeiro mês de vida. Pesquisa Veterinária Brasileira. 32(4): 357-360.

Biondo A.W., Lopes S.T.A., Kohayagawa A., Takahira R.K. & Alencar N.X. 1998. Hemograma de bovinos (Bos indicus) sadios da raça Nelore no primeiro mês de vida, criados no estado de São Paulo. Ciência Rural. 28(2): 251-256.

Birgel junior E.H., D’Angelino J.L., Benesi F.J. & Birgel E.H. 2001. Valores de referência do leucograma de bovinos da raça Jersey criados no Estado de São Paulo. Brazilian Journal of Veterinary Research Animal Science. 38(3): 136-141.

Costa J.N. 2000. Leucograma, metabolismo oxidativo dos neutrófilos, proteinograma e imunoglobulinas de bovinos da raça holandesa (Bos taurus). Influência do desenvolvimento etário e da suplementação com vitamina E (acetato de DL-alfa tocoferol). 209f. Botucatu, SP. Tese (Doutorado em Clínica Veterinária) - Programa de Pós-graduação em Clínica Veterinária, Universidade Estadual Paulista.

Costa M.C., Flaiban K.K.M.C., Coneglian M.M., Feitosa F.L.F., Balarin M.R.S. & Lisbôa J.A.N. 2008. Transferência de imunidade passiva em bezerros das raças Nelore e Limousin e proteinograma sérico nos primeiros quatro meses de vida. Pesquisa Veterinária Brasileira. 28(9): 410-416.

Costa M.C., Flaiban K.K.M.C., Coneglian M.M., Dognani R., Vettorato E.D., Balarin M.R.S. & Lisbôa J.A.N. 2008. Metabolismo oxidativo dos neutrófilos de bezerros das raças Nelore e Limousin nos primeiros quatro meses de vida. Pesquisa Veterinária Brasileira. 28(9): 431-436.

Curi P.R. 1998. Metodologia e análise da pesquisa em ciências biológicas. 2.ed. Botucatu: Gráfica e Editora Tipomic, 263p.

Delfino J.L., Barbosa V.M., Gondim C.C., Oliveira P.M., Nasciutti N.R., Oliveira R.S.B.R., Tsuruta S.A., Mundim A.V. & Saut J.P.E. 2014. Perfil bioquímico sérico de bezerros senepol nos primeiros 120 dias de idade. Semina: Ciência Agrárias. 35(3): 1341-1350.

Ebehart R.J. & Patt J.A. 1971. Plasma cortisol concentrations in newborn calves. American Journal of Veterinary Research. 32(12): 1921-1927.

Fagliari J.J., Santana A.E., Lucas F.A., Campos Filho E. & Curi P.R. 1998. Constituintes sangüíneos de bovinos recémnascidos das raças Nelore (Bos indicus) e Holandesa (Bos taurus) e de bubalinos (Bubalis bubalus) da raça Murrah. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 50(5): 253-262.

Gonçalves R.C., Peixoto A.P.C., Kuchembuck M.R., Chiacchio S.B., Kohayagawa A. & Castro A.A.P. 1993. Doenças de bezerros. Pneumonia. Aspectos clínicos e epidemiológicos na região de Botucatu. In: Resumos do XI Congresso Internacional de Medicina Veterinária em Língua Portuguesa (Salvador, Brasil). p.289.

Jain N.C. 1993. Essentials of veterinary hematology. Philadelphia: Lea & Febiger, 417p.

Kaneko J.J., Harvey J.W. & Bruss M.L. 2008. Clinical biochemistry of domestic animals. 6th edn. San Diego: Academic Press, 928p.

Machado Neto R., Packer I.V., Bonilha L.M., Figueredo L.A., Razzok A.G. & Candido J.G. 1997. Concentração de IgG sérica em bezerros das raças Nelore, Guzerá, Gir e Caracu. Efeito sobre o crescimento e a mortalidade até a desmama. Revista Brasileira de Zootecnia. 26: 920-923.

Mendonça C.L., Vieira D., Kohayahawa A., Schenk M.A.M., Madruga C.R. & Afonso J.A.B. 2003. Avaliação clínica e hematológica em bezerros Nelore infectados experimentalmente com isolados de Babesia bigemina das regiões Sudeste, Nordeste e Norte do Brasil. Pesquisa Veterinária Brasileira. 23(2): 52-60.

Naylor J.M., Kronfeld D.S., Bech-Nielsen S. & Bartholomew R.C. 1977. Plasma total protein measurement for prediction of disease and mortality in calves. Journal of the American Veterinary Medical Association. 171(7): 635-638.

Novo S.M.F., Freitas R.L., Silva C.P.C., Baldacim V.A.P., Baccili C.C., Reis J.F., Hagiwara M.K. & Gomes V. 2015. Hematological adaptation in Holstein calves during the neonatal period. Brazilian Journal of Veterinary Research and Animal Science. 52(3): 212-216.

Peixoto A.P.C., Costa J.N., Kohayagawa A., Takahira R.K. & Saito M.E. 2002. Hemograma e metabolismo oxidativo dos neutrófilos de bovinos da raça Holandesa preta e branca: influência dos fatores etários. Revista Brasileira de Saúde Produção Animal. 3(1): 16-20.

Radostits O.M., Gay C.C., Blood D.C. & Hinchcliff K.W. 2002. Clínica Veterinária: Um tratado de doenças dos bovinos, ovinos, suínos, caprinos e equinos. 9.ed. Rio de Janeiro: Guanabara Koogan, 1737p.

Rengifo S.A., Silva R.A., Botteon R.C.C.M. & Botteon P.T.L. 2010. Hemograma e bioquímica sérica auxiliar em bezerros mestiços neonatos e ocorrência de enfermidades. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 62(4): 993-997.

Rocha T.G., Franciosi C., Nociti R.P., Nogueira C.A.S. & Fagliari J.J. 2010. Hemograma e proteínas do soro sanguíneo de bezerros Canchim-Nelore e da raça Holandesa nos primeiros 30 dias de vida. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 62(5): 1250-1254.

Rocha T.G., Nociti R.P., Sampaio A.A.M. & Fagliari J.J. 2013. Hemograma e proteínas de fase aguda de bezerros sadios do nascimento aos 30 dias de idade. Pesquisa Veterinária Brasileira. 33(1): 25-31.

Soliman M.K. & Zaki K. 1967. Blood picture of Friesian heifers from birth till maturity. Zentralblatt für Veterinarmedizin, Reihe A. 13: 467-473.

Teixeira W.T., Fonteque G.V., Ramos A.F., Mariante A.S., Egito A.A., Martins V.V., Saito M.E. & Fonteque J.H. 2012. Transfer of passive immunity and serum proteinogram in the first six months of life of Criollo Lageano and Black and White Holstein calves. Pesquisa Veterinária Brasileira. 32(10): 980-986.

Tornquist S.J. & Rigas J. 2010. Interpretation of ruminant leukocyte responses. In: Weiss D.J. & Wardrop K.J. (Eds). Schalm´s veterinary hematology. 6th edn. Ames: Wiley-Blackwell, pp.307-313.




DOI: https://doi.org/10.22456/1679-9216.81155

Copyright (c) 2018 Jackson Schade, Renato May Rossi, Mere Erika Saito, Graziela Vieira Fonteque, Thiago Rinaldi Muller, Joandes Henrique Fonteque

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.