Immunohistochemical detection of GFAP and TGF ?1 in C57Bl6 mice during acute vesicular stomatitis virus encephalitis

Authors

  • Gisele Fabrino Machado UNESP
  • Paulo Cesar Maiorka USP
  • Cinthia Graziela Candioto UNESP
  • Luciana Mari Ushiro Ieiri UNESP
  • Antonio Carlos Alessi UNESP

DOI:

https://doi.org/10.22456/1679-9216.15075

Keywords:

Astrócitos, Gliose, TGF-b1, Vesicular estomatite vírus

Abstract

Activation of astrocytes or astrogliosis is a prominent component of the inflammatory response and an indicator of injury in the brain. These astrocytes produce a large array of inflammatory mediators, growth and neuroprotective factors. This study was an investigation from astrocyte (GFAP) response and TGF-

 

?1 involvement during VSV acute encephalitis using immunohistochemistry to verify relation between astrocytes and TGF-?1. Animals developed symptoms around 6th day after VSV inoculation. Viral proteins were mainly detected at olfactory bulb, ventricular cell layer and disseminated to hippocampus, mesencephalon and diecephalon areas and brain stem. Also at 6th day post inoculation GFAP and TGF-?1 staining was observed in good association with virus-detected areas of brain. However, in mice with severe symptoms we observed reduction in the intensity of GFAP labeling at the same areas where TGF-?1 upregulation was observed. These areas show correlation with areas of necrosis and where are astrocytes with degenerative aspect. We observed TGF-?1 staining in damaged astrocytes, suggesting an effort of those cells in controlling inflammation in acute phase of VSV encephalitis.

Downloads

Download data is not yet available.

Author Biographies

Gisele Fabrino Machado, UNESP

Paulo Cesar Maiorka, USP

 

 

USP – FMVZ – São Paulo, Department of Pathology.

Cinthia Graziela Candioto, UNESP

 

 

UNESP – São Paulo State University, School of Veterinary Medicine, Araçatuba, Department of Clinical Surgery and Animal Reproduction.

Luciana Mari Ushiro Ieiri, UNESP

 

 

UNESP – São Paulo State University, School of Veterinary Medicine, Araçatuba, Department of Clinical Surgery and Animal Reproduction.

Antonio Carlos Alessi, UNESP

 

 

UNESP-FCAV-Jaboticabal, Department of Veterinary Pathology.

Published

2018-06-27

How to Cite

Machado, G. F., Maiorka, P. C., Candioto, C. G., Ieiri, L. M. U., & Alessi, A. C. (2018). Immunohistochemical detection of GFAP and TGF ?1 in C57Bl6 mice during acute vesicular stomatitis virus encephalitis. Acta Scientiae Veterinariae, 34(1), 83–88. https://doi.org/10.22456/1679-9216.15075

Issue

Vol. 34 No. 1 (2006)

Section

Articles

License

This journal provides open access to all of its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Such access is associated with increased readership and increased citation of an author's work. For more information on this approach, see the Public Knowledge Project and Directory of Open Access Journals.

We define open access journals as journals that use a funding model that does not charge readers or their institutions for access. From the BOAI definition of "open access" we take the right of users to "read, download, copy, distribute, print, search, or link to the full texts of these articles" as mandatory for a journal to be included in the directory.

La Red y Portal Iberoamericano de Revistas Científicas de Veterinaria de Libre Acceso reúne a las principales publicaciones científicas editadas en España, Portugal, Latino América y otros países del ámbito latino