Evaluation of Platelet Indices and Complete Blood Count in Canine Mammary Tumors
DOI:
https://doi.org/10.22456/1679-9216.114293Abstract
Background: Malignant mammary tumors in humans and bitches cause hematological disorders such as anemia, erythrocytosis, thrombocytosis, hyperproteinemia, and leucopenia. Novel studies have been conducted on the predictive and prognostic values of platelet (PLT) indices in human breast cancer (HBC). However, there is little information about the alterations in hematological parameters in canine mammary tumors (CMTs). The aims of this study were to evaluate the platelet indices and complete blood count (CBC) parameters in bitches with and without mammary tumor and to assess the above mentioned parameters with regard to histological tumor types and grades.
Materials, Methods & Results: A total of 71 bitches were enrolled in this study. The bitches in the study group were divided into 2 groups which consisted of malignant epithelial mammary tumors (group EMT; n = 43) and malignant mixed mammary tumors (group MMT; n = 12). Control group (group C) consisted of clinically and gynaecologically healthy 16 bitches. Blood samples were obtained to perform the CBC and PLT indices analysis. Histopathological examinations were carried out under a light microscope. Histological tumor types and malignancy grades were classified. The bitches with mammary tumor showed significantly increased PLT values and decreased hematocrit (HCT), hemoglobin (HGB) and mean corpuscular hemoglobin (MCH) values versus the healthy ones, regardless of the tumor type. However, in comparisons with the group C, mean platelet volume (MPV) and mean corpuscular hemoglobin concentration (MCHC) values were different only in the group MMT, while plateletcrit (PCT) and mean corpuscular volume (MCV) values were different only in the group EMT. Also white blood cell (WBC), PLT and PCT values were higher than the referenced laboratory ranges in grade 3 tumors. In the presented study, MPV was considerably correlated with PLT, platelet distribution width (PDW) and PCT. Also, PCT and PLT had high sensitivity and specificity to distinct EMT and MMT from the healthy bitches.
Discussion: Microcytic and hypochromic anemia occurs due to the decrease in the amount of HGB. Levels of MCV, MCH, and MCHC in the HBC group were reported to be significantly lower than in humans without breast cancer. Although anemia did not occur in EMT and MMT groups, obtained significances in the HCT, HGB, MCV, MCH, and MCHC levels between the bitches with and without mammary tumor were in line with the previous reports. In this study, WBC levels in grade 3 tumors were significantly higher than grade1 tumors (P < 0.05). Whereas levels of WBC in grade 1 and grade 2 tumors were in referenced laboratory ranges, it was higher in grade 3. Increased level of WBC in grade 3 was supposed to be due to the rise in malignancy as previously reported. Thrombocytosis was detected in 48.83% and 41.66% of the bitches in EMT and MMT groups, respectively. The higher percentage of CMTs with thrombocytosis in this study might be due to the difference in referenced upper limit of PLT in previous studies. The elapsed time between tumor formation and clinical presentation could be another influencing factor. Although PLT and PCT values were not significant according to the histological grading in this study, both parameters were found to be higher in grade 3 than the normal reference values. Further studies conducted with higher populations may lead the differences in these parameters to significance. With the support of further studies, alterations in the above mentioned parameters in bitches may contribute in the diagnosis process, management of treatment and may constitute an easy way to have an idea about the prognosis of mammary tumors.
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References
Abdelmegeed S.M. & Mohammed S. 2018. Canine Mammary Tumors as a Model for Human Disease. Oncology Letters. 15(6): 8195-8205. DOI: 10.3892/ol.2018.8411
Alsweedan S.A., Al-Shurman A. & Mahmoud A.S. 2008. Diagnostic Value of Platelet Indices in Children with Leukemia. Journal of Pediatric Hematology/Oncology. 30(12): 953-955. DOI: 10.1097/MPH.0b013e318182e7a9
Andreasen E.B., Tranholm M., Wiinberg B., Markussen B. & Kristensen A. 2012. Haemostatic Alterations in a Group of Canine Cancer Patients Are Associated with Cancer Type and Disease Progression. Acta Veterinaria Scandinavica. 54(1): 3. DOI: 10.1186/1751-0147-54-3
Anjos D.S., Santilli J., Vital A.F., de Oliveira J.R., Sobreira M.F.R., Magalhães G.M., Calazans S.G. & Fonseca-Alves C.E. 2018. Evaluation of Hemostatic Parameters in Tumor-Bearing Dogs. Acta Scientiae Veterinariae. 46(1): 8. DOI: 10.22456/1679-9216.89004
Aydın D., Olgun Erdikmen D., Ülgen S., Demirutku A. & Durmuş D. 2011. Paraneoplastic Syndromes in Cats and Dogs. Journal of Faculty of Veterinary Medicine, Erciyes University. 8(2): 127-137.
Ayhan A., Bozdag G., Taskiran C., Gultekin M., Yuce K. & Kucukali T. 2006. The Value of Preoperative Platelet Count in the Prediction of Cervical Involvement and Poor Prognostic Variables in Patients with Endometrial Carcinoma. Gynecologic Oncology. 103(3):902–905. DOI: 10.1016/j.ygyno.2006.05.034
Bambace N.M. & Holmes C.E. 2011. The Platelet Contribution to Cancer Progression. Journal of Thrombosis and Haemostasis. 9(2): 237-249. DOI: 10.1111/j.1538-7836.2010.04131.x
Baştan I. 2013. Paraneoplastic Syndrome in Dogs. Dicle Üniversitesi Veteriner Fakültesi Dergisi. 1(4): 19-24.
Benavente M.A., Bianchi C. & Aba M. 2016. Canine Mammary Tumors: Risk Factors, Prognosis and Treatments. Journal of Veterinary Advances. 6(8): 1291-1300. DOI: 10.5455/jva.20160916045115
Campos C.B., Lavalle G.E., Ligorio S.F., Monteiro L.N., Amorim R.L. & Cassali G.D. 2017. Thalidomide Treatment in a Canine Mammary Gland Carcinosarcoma Presenting Pulmonary Metastasis. Advances in Animal and Veterinary Sciences. 5(3):120-126. DOI: 10.14737/journal.aavs/2017/5.3.120.126
Cassali G.D., Bertagnolli A.C., Ferreira E., Damasceno K.A, Gamba C.O & de Campos C.B 2012. Canine Mammary Mixed Tumours: A Review. Veterinary Medicine International. 2012: 274608. DOI: 10.1155/2012/274608
Gaspar B.L., Sharma P. & Das R. 2015. Anemia in Malignancies: Pathogenetic and Diagnostic Considerations. Hematology. 20(1): 18-25. DOI: 10.1179/1607845414Y.0000000161
Giraldo N.A., Becht E., Vano Y., Sautès‑Fridman C. & Fridman W.H. 2015. The Immune Response in Cancer: From Immunology to Pathology to Immunotherapy. Virchows Archiv: An International Journal of Pathology. 467(2): 127-135. DOI: 10.1007/s00428-015-1787-7
Goldschmidt M., Pena L., Rasotto R. & Zappulli V. 2011. Classification and Grading of Canine Mammary Tumors. Veterinary Pathology. 48(1): 117-131. DOI: 10.1177/0300985810393258
Gu M., Zhai Z., Huang L., Zheng W., Zhou Y., Zhu R., Shen F. & Yuan C. 2016. Pre-Treatment Mean Platelet Volume Associates with Worse Clinicopathologic Features and Prognosis of Patients with Invasive Breast Cancer. Breast Cancer. 23(5): 752-760. DOI: 10.1007/s12282-015-0635-6.
Inagaki N., Kibata K., Tamaki T., Shimizu T. & Nomura S. 2014. Prognostic Impact of the Mean Platelet Volume/Platelet Count Ratio in Terms of Survival in Advanced Non-Small Cell Lung Cancer. Lung Cancer. 83(1): 97-101. DOI: 10.1016/j.lungcan.2013.08.020
Jurasz P., Alonso‐Escolano D. & Radomski M.W. 2004. Platelet–Cancer Interactions: Mechanisms and Pharmacology of Tumour Cell‐Induced Platelet Aggregation. British Journal of Pharmacology. 143(7): 819-826. DOI: 10.1038/sj.bjp.0706013
Karateke A., Kaplanoglu M. & Baloglu A. 2015. Relations of Platelet Indices with Endometrial Hyperplasia and Endometrial Cancer. Asian Pacific Journal of Cancer Prevention. 16(12): 4905-4908. DOI: 10.7314/apjcp.2015.16.12.4905
Koenhemsi L. 2019. Determination of Platelet Count and Platelet Indices in Canine Parvoviral Enteritis. Medical Science and Discovery. 6(2): 24-26. DOI: 10.17546/msd.522081
Koenhemsi L., Uçmak Z.G., Uçmak M. & Or M.E. 2020. Platelet Indices in Dogs and Cats with Pyometra. Revue Vétérinaire Clinique. 55(4): 147-150. DOI: 10.1016/j.anicom.2020.07.002
Lallo M.A., Ferrarias T.M., Stravino A., Rodriguez J.F.M. & Zucare R.L.C. 2016. Hematologic Abnormalities in Dogs Bearing Mammary Tumors. Revista Brasileira de Ciência Veterinária. 23(1/2): 3-8. DOI: 10.4322/rbcv.2016.020
Lu Y., Cui M., Liang Z., Wang W., Jiang M., Xu M., Wu M., Shen M., Li W., Gao Y., Lian L. & Duan W. 2019. Prognostic Values of Platelet-Associated Indicators in Advanced Breast Cancer. Translational Cancer Research. 8(4): 1326-1335. DOI: 10.21037/tcr.2019.07.28
Ma X., Wang Y., Sheng H., Tian W., Qi Z., Teng F. & Xue F. 2014. Prognostic Significance of Thrombocytosis, Platelet Parameters and Aggregation Rates in Epithelial Ovarian Cancer. The Journal of Obstetrics and Gynaecology Research. 40(1): 178-183. DOI: 10.1111/jog.12151
Mantas D., Kostakis I.D., Machairas N. & Markopoulos C. 2016. White Blood Cell and Platelet Indices as Prognostic Markers in Patients with Invasive Ductal Breast Carcinoma. Oncology Letters. 12(2): 1610-1614. DOI: 10.3892/ol.2016.4760
Okuturlar Y., Gunaldi M., Tiken E.E., Oztosun B., Inan Y.O., Ercan T., Tuna S., Kaya A.O., Harmankaya O. & Kumbasar A. 2015. Utility of Peripheral Blood Parameters in Predicting Breast Cancer Risk. Asian Pacific Journal of Cancer Prevention. 16(6): 2409-2412. DOI: 10.7314/apjcp.2015.16.6.2409.
Oncel M., Kiyici A., Oncel M., Sunam G.S., Sahin E. & Adam B. 2015. Evaluation of Platelet Indices in Lung Cancer Patients. Asian Pacific Journal of Cancer Prevention. 16(17): 7599-7602. DOI: 10.7314/apjcp.2015.16.17.7599.
Plumb D.C. 2008. Reference laboratory ranges. In: Plumb’s Veterinary Drug Handbook. Ames: Blackwell Publishing Professional, pp.1394-1395.
Rajizadeh A., Mozaffari-Khosravi H., Zavar-reza J. & Shiryazdi SM. 2017. Comparison of Hematological Parameters, Iron Levels, and Oxidative Stress in Women with and without Breast Cancer: A Case- Control Study. Medical Journal of the Islamic Republic of Iran. 31: 114. DOI: 10.14196/mjiri.31.114
Reidl J., Pabinger I. & Ay C. 2014. Platelet in Cancer and Thrombosis. Hamostaseologie. 34(1): 55-62. DOI: 10.5482/HAMO-13-10-0054
Saad E.S., Milley K.M., Al-Khan A.A., Nimmo J.S., Bacci B., Tayebi M., Day M.J., Richardson S.J. & Danks J.A. 2019. Canine Mixed Mammary Tumour as a Model for Human Breast Cancer with Osseous Metaplasia. Journal of Comparative Pathology. 156(4): 352-365. DOI: 10.1016/j.jcpa.2017.03.005
Sahabi K., Selvarajah G.T., Abdullah R., Cheah Y.K. & Tan G.C. 2018. Comparative Aspects of MicroRNA Expression in Canine and Human Cancers. Journal of Veterinary Science. 19(2): 162-171. DOI: 10.4142/jvs.2018.19.2.162
Sharma D. & Sing G. 2017. Thrombocytosis in Gynecological Cancers. Journal of Cancer Research and Therapeutics. 13(2): 193-197. DOI: 10.4103/0973-1482.189234
Sorenmo K. 2013. Canine Mammary Gland Tumors. Veterinary Clinics: Small Animal Practice. 33(3): 573-596. DOI: 10.1016/s0195-5616(03)00020-2
Stockhaus C., Kohn B., Rudolph R., Brunnberg L. & Giger U. 1999. Correlation of Haemostatic Abnormalities with Tumour Stage and Characteristics in Dogs with Mammary Carcinoma. The Journal of Small Animal Practice. 40(7): 326-331. DOI: 10.1111/j.1748-5827.1999.tb03090.x
Takeuchi H., Noda D., Abe M., Anami K., Miyawaki M., Osoegawa A. & Sugio K. 2020. Evaluating the Platelet Distribution Width-to-Plateletcrit Ratio as a Prognostic Marker for Patients with Breast Cancer. Anticancer Research. 40(7): 3947-3952. DOI: 10.21873/anticanres.14386
Taucher S., Salat A., Gnant M., Kwasny W., Mlineritsch B., Menzel R.C., Schmid M., Smola M.G., Stierer M., Tausch C., Galid A., Steger G. & Jakesz R. 2003. Impact of Pretreatment Thrombocytosis on Survival in Primary Breast Cancer. Thrombosis and Haemostasis. 89(6): 1098-1106. PMID: 12783124
Templeton A.J., McNamara M.G., Šeruga B., Vera‑Badillo F.E., Aneja P., Ocaña A., Leibowitz‑Amit R., Sonpavde G., Knox J.J., Tran B., Tannock I.F. & Amir E. 2014. Prognostic Role of Neutrophil‑to‑Lymphocyte Ratio in Solid Tumors: A Systematic Review and Meta‑Analysis. Journal of the National Cancer Institute. 106(6): 1-11. DOI: 10.1093/jnci/dju124
Varlotto J. & Stevenson M.A. 2005. Anemia, Tumor Hypoxemia, and the Cancer Patient. International Journal of Radiation Oncology, Biology, Physics. 63(1): 25-36. DOI: 10.1016/j.ijrobp.2005.04.049.
Zhu X., Cao Y., Lu P., Kang Y., Lin Z., Hao Y. & Song Y. 2018. Evaluation of Platelet Indices as Diagnostic Biomarkers for Colorectal Cancer. Scientific Reports. 8(1): 11814. DOI: 10.1038/s41598-018-29293-x
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