Is Bluetongue Virus a Risk Factor for Reproductive Failure in Tropical Hair Sheep in Brazil?

Authors

  • Huber Rizzo Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil. http://orcid.org/0000-0003-1559-6962
  • Mario Felipe Alvarez Balaro Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Ana Carolina Diniz Matos Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Zélia Inês Portela Lobato Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.
  • Lilian Gregory Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.

DOI:

https://doi.org/10.22456/1679-9216.112591

Abstract

Background: Bluetongue is a vector-borne viral disease transmitted by midges from the genus Culicoides. The disease can infect most of the ruminant and camelid species, but the severe disease is most often seen in european wool and mutton sheep breeds. In this sense, there is a gap in the knowledge on BTV infection in hair sheep breeds from tropical zones. Thus, this study aimed at establishing whether exposure to BTV is a risk factor for reproductive failure in Santa Inês ewes, a hair sheep breed, reared under tropical conditions in Brazil.

Materials, Methods & Results: A retrospective cross-sectional study was carried out in sheep farms in São Paulo state, Brazil, after the rainy season. Serum samples from 110 Santa Inês ewes with a history of reproductive disorders, in the last 6 months, which were included: abortion, premature birth, stillbirth, retention of placenta, infertility, estrus repetition, fetal malformation, weak lamb birth and neonatal death were collected. The presence of antibodies against BTV was assessed by agar gel immunodiffusion method (AGID). Serology to the infectious agents Brucela ovis, Lepstopira spp., Toxoplasma gondii, Neospora caninum and Campylobacter sp. were also assessed. Bivariate associations between the outcome and individual explanatory variables were assessed using the Fisher's exact test. Abortion was the most common reproductive disorder (53%; 74/139) observed, followed by estrus repetition (12%; 17/139) and infertility (11%; 15/139). Other disorders related to the conceptus totaled nearly one fourth of the reported disorders. A total of 20% (22/110) of the ewes were seropositive to BTV. A higher frequency of BTV seropositive than BTV seronegative ewes with a history of abortion was found. Also, abortion with seroreactivity to BTV was tested for prevalence ratio that showed 1.38 [95% CI 1.10-1.74; P = 0.030]. With regards to the abortion involvement of other infectious diseases associated with the seropositive ewes to BTV, more than a half of ewes (53%; 10/19) were solely seropositive for BTV.

Discussion: In the current study, it was detected 20% (22/110) of seropositive ewes to BTV. These findings demonstrated that even though the BTV has been considered endemic in tropical countries such as Brazil, there are regions or microclimates in which the virus cannot be present or in varied prevalence. The history of abortion was identified as the potential factor associated with BTV seropositivity in Santa Ines ewes. Equally, the differential diagnosis for other infectious agents related to abortion demonstrated the unique presence of antibodies against BTV in more than half of all cases. Other studies with native sheep flocks in Iran and Nepal also demonstrated a strong positive correlation between abortion history and seropositivity for BTV. Thus, it is possible that in other continents of the world, under tropical conditions, the virus does not behave the same asymptomatic infection such as have been reported for native sheep breeds in Africa. In conclusion, it was demonstrated that one-fifth animals were positive for antibodies against BTV clearly implying the viral spreading in the local hair sheep flocks. These findings highlight the importance of surveillance related to BTV in endemic areas. Therefore, it is recommended to strengthen the surveillance system for BTV within Brazil and to educate farmers about the management and control of this disease.

 

 

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Author Biography

Huber Rizzo, Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil.

Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco, Recife, PE

References

Balaro M.F.A., Lima M.D.S., Fava C.D., Oliveira G.R., Pituco E.M. & Brandão F.Z. 2014. Outbreak of Bluetongue virus serotype 4 in dairy sheep in Rio de Janeiro, Brazil. Journal of Veterinary Diagnostic Investigation. 26(4): 567-570.

Bastos L.S., Oliveira R.V.C. & Velasque L.S. 2015. Obtaining adjusted prevalence ratios from logistic regression models in cross-sectional studies. Cadernos de Saúde Pública. 31(3): 487-495.

Camargo M.E. 1974. Improved technique of indirect immunofluorescence for serological diagnosis of toxoplasmosis. Revista do Instituto de Medicina Tropical de São Paulo. 6: 117-118.

Costa J.R.R. 2000. Produção e padronização de antígeno para língua azul e prevalência nas mesorregiões Sudoeste e Sudeste do Rio Grande do Sul, 1999. 55f. Belo Horizonte, MG. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Minas Gerais.

Dubey J.P. 1998. Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues. Veterinary Parasitology. 74(1): 75-77.

Faine S. 1982. Guidelines for the control of leptospirosis. 2nd edn. Geneva: WHO Offset Publication, 171p.

Filho L.F.C. Cunha, Sbizera M.C.R., Barreto J.V.P., Pituco E.M., Lorenzetti E.L., Michele P., Constantino T.H. & Matias B.F. 2019. Bluetongue disease in sheep: a review. Arquivos do Instituto Biológico. 86: e1342018.

Gaire T., Karki S., Dhakal I., Khanal D., Joshi N., Sharma B. & Bowen R.A. 2014. Cross-sectional serosurvey and associated factors of bluetongue virus antibodies presence in small ruminants of Nepal. BMC Research Notes. 7: 691.

Gerdes G.H. 2004. A South African overview of the virus, vectors, surveillance and unique features of bluetongue. Veterinaria Italiana. 40(3): 39-42.

Guimarães L.L.B., Rosa J.C.C., Matos A.C.D., Cruz R.A.S., Guedes M.I.M.C., Dorella F.A., Figueiredo H.C.P., Pavarini S.P., Sonne L., Lobato Z.I.P. & Driemeier D. 2017. Identification of bluetongue virus serotypes 1, 4, and 17 co-infections in sheep flocks during outbreaks in Brazil. Research in Veterinary Science. 113: 87-93.

Kardjadj M., Kouidri B., Metref D., Luka P. D. & Ben-Mahdi M. H. 2016. Abortion and various associated risk factors in small ruminants in Algeria. Preventive Veterinary Medicine. 123: 97-101.

Legisa D.M., Gonzalez F.N. & Dus Santos M.J. 2014. Bluetongue virus in South America, Central America and the Caribbean. Virus Research. 182: 87-94.

Lima P.A., Utiumi K.U., Nakagaki K.Y.R., Biihrer D.A., Albuquerque A.S., Souza F.R., Matos A.C.D., Lobato Z.I.P., Driemeier D., Peconick A.P., Varaschin M.S. & Raymundo D.L. 2016. Diagnoses of ovine infection by the serotype-4 bluetongue virus on Minas Gerais, Brazil. Acta Scientiae Veterinariae. 44(supl.1): 1-5.

Maclachlan N.J., Drew C.P., Darpel K.E. & Worwa G. 2009. The pathology and pathogenesis of bluetongue. Journal of Comparative Pathology. 141(1): 1-16.

Maclachlan N.J. & Osburn B.I. 2006. Impact of bluetongue virus infection on the international movement and trade of ruminants. Journal of the American Veterinary Medical Association. 228(9): 1346-1349.

Matos A.C.D., Balaro M.F.A., Guedes M.I.M.C., Costa E.A., Rosa J.C.C., Costa A.G., Brandão F.Z. & Lobato Z.I.P. 2016. Epidemiology of a Bluetongue outbreak in a sheep flock in Brazil. Veterinaria Italiana. 52(3-4): 325-331.

Mohammadi A., Tanzifi P. & Nemati Y. 2012. Seroepidemiology of bluetongue disease and risk factors in small ruminants of Shiraz suburb, Fars province Iran. Tropical Biomedicine. 29(4): 632-637.

Najarnezhad V. & Mahin V.R. 2013. Seroepidemiology of bluetongue disease in small ruminants of northeast Iran. Asian Pacific Journal of Tropical Biomedicine. 3(6): 492-495.

Nogueira A.H.C., Pituco E.M., Stefano E., Curci V.C.L.M. & Cardoso T.C. 2009. Detection of antibodies against the bluetongue virus in sheep in the region of Araçatuba, São Paulo, Brazil. Ciência Animal Brasileira. 10(4): 1271-1276.

Prasad G., Jain N.C., Mahajan N.K. & Vasudevan B. 1987. Prevalence of bluetongue precipitating antibodies in different domestic animals. Indian Journal of Animal Sciences. 57(6): 522-524.

Rizzo H., Gregory L., Beraldi F., Carvalho A.F. & Paulim L.M. 2014. Ocorrência de anticorpos anti-Brucella ovis em ovinos com histórico de distúrbios reprodutivos no estado de São Paulo, Brasil. Arquivos do Instituto Biológico. 81(2): 99-106.

Rushton J. & Lyons N. 2015. Economic impact of bluetongue: a review of the effects on production. Veterinaria Italiana. 51(4): 401-406.

Saegerman C., Bolkaert B., Baricalla C., Raes M., Wiggers L., Leeuw I., Vandenbussche F., Zimmer J.Y. Haubruge E., Cassart D., Clercq K. & Kirschvink N. 2001. The impact of naturally-occurring, trans-placental bluetongue virus serotype-8 infection on reproductive performance in sheep. Veterinary Journal. 187(2): 72-80.

Santolaria P., Palacin I. & Yaniz J.L. 2011. Management factors affecting fertility in sheep, In: Manafi M. (Ed). Artifical Insemination in Farm Animals. Tamil Nadu: Intech Publisher, pp.167-190.

Saminathan M., Singh K. P., Khorajiya J. H., Dinesh M., Vineetha S., Maity M., Rahman A.F., Misri J., Malik Y.S., Gupta V.K., Singh R.K. & Dhama K. 2020. An updated review on Bluetongue virus: Epidemiology, pathobiology, and advances in diagnosis and control with special reference to India. Veterinary Quarterly. 40(1): 258-321.

Saminathan M., Singh K.P., Vineetha S., Maity M., Biswas S.K., Manjunathareddy G.B., Chauhan H.C., Milton A.A.P., Ramakrishnan M.A., Maan S., Maan N.S., Hemadri D., Chandel B.S., Gupta V.K. & Mertens P.P.C. 2020. Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid-gestation. Scientific Reports. 10(1): 2164.

Scarcelli E., Genovez M.E., Cardoso M.V., Souza M.C.A.M., Grasso L.M.P.S., Souza C.A.I. & Torres A.P. 1998. Avaliação do potencial de disseminação de Campylobacter spp. por diferentes espécies animais. Arquivos do Instituto Biológico. 65(1): 55-61.

Sbizera M.C.R., Cunha Filho L.F.C., Lunardi M., Pertile S.F.N., Patelli T.H.C., Barreto J.V.P. & Pituco E.M. 2020. Detection of bluetongue virus antibodies in sheep from Paraná, Brazil. Semina: Ciências Agrárias. 41(3): 879-886.

Sohail T., Yaqub T., Abbas T., Rabbani M., Nazir J., Maqbool S.M., Yaqub S., Habib M., Ul-Rahman A., Mukhtar N., Shahbaz M., Zahoor M.Y. & Shabbir M.Z. 2019. Seroprevalence of Bluetongue Virus in small and large ruminants in Punjab province, Pakistan. Acta Tropica. 189: 22-29.

Venditti L.L.R. 2009. Infecção pelo vírus da língua azul em ovinos e bovinos na região Sudeste do Brasil. 77f. São Paulo, SP. Dissertação (Mestrado em Medicina Veterinária) - Programa de Pós-graduação em Sanidade, Segurança Alimentar e Ambiental no Agronegócio, Instituto Biológico de São Paulo.

World Organization for Animal Health (OIE). 2009. Bluetongue virus and epizootic haemorrhagic disease. In: Manual of diagnostic tests and vaccines for terrestrial animals. Chapter 2.1.3. Paris: OIE, pp.158-174.

Published

2021-01-01

How to Cite

Rizzo, H., Balaro, M. F. A., Matos, A. C. D., Lobato, Z. I. P., & Gregory, L. (2021). Is Bluetongue Virus a Risk Factor for Reproductive Failure in Tropical Hair Sheep in Brazil?. Acta Scientiae Veterinariae, 49. https://doi.org/10.22456/1679-9216.112591

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